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Comparative RNA-Seq analysis of Nicotiana benthamiana in response to Phytophthora parasitica infection

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Abstract

Phytophthora parasitica causes serious damage to a broad spectrum of agriculturally important crops and natural ecosystems. To investigate plant responses to P. p arasitica, differential gene expressions between inoculated and mock-treated Nicotiana benthamiana leaves were analyzed by RNA-Seq approach. A total of 5375 and 3614 N. b enthamiana genes were found to be upregulated and downregulated, respectively. Infection with P. p arasitica triggered massive metabolic reprogramming in the inoculated tissues. Genes related to photosynthesis, starch biosynthesis, and nitrogen assimilation were suppressed while sucrose degrading genes were induced. Notably, plant defense responses were activated, reflected by larger number of upregulated JA and ET signaling genes, receptor-like kinases, pathogenesis-related genes, and transcription factors. Collectively, these results provide broad insights into N. b enthamiana defense mechanisms against P. p arasitca and advance our understanding of plant-Phytophthora interactions.

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Acknowledgments

This research was supported by grants from the National Natural Science Foundation of China (31501589) and China Postdoctoral Science Foundation (2015M571769).

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Authors and Affiliations

  1. Department of Plant Pathology, Nanjing Agricultural University, Nanjing, 210095, China

    Danyu Shen, Chunyue Chai, Lina Ma, Meixiang Zhang & Daolong Dou

  2. College of Life Science and Technology, Nanyang Normal University, Nanyang, 473061, China

    Chunyue Chai

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  1. Danyu Shen
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  2. Chunyue Chai
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  3. Lina Ma
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  4. Meixiang Zhang
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  5. Daolong Dou
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Correspondence to Daolong Dou.

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Shen, D., Chai, C., Ma, L. et al. Comparative RNA-Seq analysis of Nicotiana benthamiana in response to Phytophthora parasitica infection. Plant Growth Regul 80, 59–67 (2016). https://doi.org/10.1007/s10725-016-0163-1

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  • DOI: https://doi.org/10.1007/s10725-016-0163-1

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