Skip to main content

Advertisement

SpringerLink
Log in

Wheat (Triticum aestivum) zinc finger-containing glycine-rich RNA-binding protein TaRZ1 affects plant growth and defense response in Arabidopsis thaliana

  • Original Paper
  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

Although the importance of zinc finger-containing glycine-rich RNA-binding proteins (RZs) during plant growth and stress adaptation has been determined in diverse plant species, the function and importance of RZs in wheat (Triticum aestivum) are largely unknown. Here, functional role of wheat TaRZ1 in the growth and defense response was evaluated using transgenic Arabidopsis thaliana. Ectopic expression of TaRZ1 in Arabidopsis resulted in retarded plant growth and reduced seed yield under normal growth conditions. Moreover, TaRZ1-expressing transgenic plants displayed increased resistance against the pathogenic bacteria Pseudomonas syringae DC3000. Microarray analysis showed that many genes related to plant growth, metabolism, and stress responses are differently up- or down-regulated in Arabidopsis by TaRZ1 expression. Taken together, these results reveal that wheat TaRZ1 has the ability to inhibit the growth of pathogenic bacteria and Arabidopsis, and suggest that TaRZ1 plays diverse roles in the growth and defense of plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Albà MM, Pagès M (1998) Plant proteins containing the RRM-recognition motif. Trends Plant Sci 3:15–21

    Article  Google Scholar 

  • Baek D, Pathange P, Chung JS, Jiang J, Gao L, Oikawa A, Hirai MY, Saito K, Pare PW, Shi H (2010) A stress-inducible sulphotransferase sulphonates salicylic acid and confers pathogen resistance in Arabidopsis. Plant, Cell Environ 33:1383–1392

    CAS  Google Scholar 

  • Bartsch M, Gobbato E, Bednarek P, Debey S, Schultze JL, Bautor J, Parker JE (2006) Salicylic acid-independent ENHANCED DISEASE SUSCEPTIBILITY1 signaling in Arabidopsis immunity and cell death is regulated by the monooxygenase FMO1 and the Nudix hydrolase NUDT7. Plant Cell 18:1038–1051

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Bednarek P, Pislewska-Bednarek M, Svatos A, Schneider B, Doubsky J, Mansurova M, Humphry M, Consonni C, Panstruga R, Sanchez-Vallet A, Molina A, Schulze-Lefert A (2009) A glucosinolate metabolism pathway in living plant cells mediates broad-spectrum antifungal defense. Science 323:101–106

    Article  CAS  PubMed  Google Scholar 

  • Bove J, Kim CY, Gibson CA, Assmann SM (2008) Characterization of wound responsive RNA-binding proteins and their splice variants in Arabidopsis. Plant Mol Biol 67:71–88

    Article  CAS  PubMed  Google Scholar 

  • Brotman Y, Lisec J, Méret M, Chet I, Willmitzer L, Viterbo A (2012) Transcript and metabolite analysis of the Trichoderma induced systemic resistance response to Pseudomonas syringae in Arabidopsis thaliana. Microbiology 158:139–146

    Article  CAS  PubMed  Google Scholar 

  • Carles CC, Lertpiriyapong K, Reville K, Fletcher JC (2004) The ULTRAPETALA1 gene functions early in Arabidopsis development to restrict shoot apical meristem activity and acts through WUSCHEL to regulate floral meristem determinacy. Genetics 167:1893–1903

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Demianski AJ, Chung KM, Kunkel BN (2012) Analysis of Arabidopsis JAZ gene expression during Pseudomonas syringae pathogenesis. Mol Plant Pathol 13:46–57

    Article  CAS  PubMed  Google Scholar 

  • Dong J, Kim ST, Lord EM (2005) Plant acyanin plays a role in reproduction in Arabidopsis. Plant Physiol 138:778–789

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Dong J, MacAlister CA, Bergmann DC (2009) BASL controls asymmetric cell division in Arabidopsis. Cell 137:1320–1330

    Article  PubMed Central  PubMed  Google Scholar 

  • Dreyfuss G, Matunis MJ, Pinol-Roma S, Burd CG (1993) HnRNP proteins and the biogenesis of mRNA. Ann Rev Biochem 62:289–321

    Article  CAS  PubMed  Google Scholar 

  • Facette MR, Smith LG (2012) Division polarity in developing stomata. Curr Opin Plant Biol 15:585–592

    Article  PubMed  Google Scholar 

  • Fletcher JC (2001) The ULTRAPETALA gene controls shoot and floral meristem size in Arabidopsis. Development 128:1323–1333

    CAS  PubMed  Google Scholar 

  • Fu ZQ, Guo M, Jeong BR, Tian F, Elthon TE, Cerny RL, Staiger D, Alfano JR (2007) A type III effector ADP-ribosylates RNA-binding proteins and quells plant immunity. Nature 447:284–288

    Article  CAS  PubMed  Google Scholar 

  • Iven T, König S, Singh S, Braus-Stromeyer SA, Bischoff M, Tietze LF, Braus GH, Lipka V, Feussner I, Dröge-Laser W (2012) Transcriptional activation and production of tryptophan-derived secondary metabolites in Arabidopsis roots contributes to the defense against the fungal vascular pathogen Verticillium longisporum. Mol Plant 5:1389–1402

    Article  CAS  PubMed  Google Scholar 

  • Jeong BR, Lin Y, Joe A, Guo M, Korneli C, Yang H, Wang P, Yu M, Cerny RL, Staiger D, Alfano JR, Xu Y (2011) Structure function analysis of an ADP-ribosyltransferase type III effector and its RNA-binding target in plant immunity. J Biol Chem 286:43272–43281

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Jung HJ, Park SJ, Kang H (2013) Regulation of RNA metabolism in plant development and stress responses. J Plant Biol 56:123–129

    Article  CAS  Google Scholar 

  • Kang H, Park SJ, Kwak KJ (2013) Plant RNA chaperones in stress response. Trends Plant Sci 18:100–106

    Article  CAS  PubMed  Google Scholar 

  • Kim YO, Kang H (2006) The role of a zinc finger-containing glycine-rich RNA binding protein during the cold adaptation process in Arabidopsis thaliana. Plant Cell Physiol 47:793–798

    Article  PubMed  Google Scholar 

  • Kim YO, Kim JS, Kang H (2005) Cold-inducible zinc finger-containing glycine-rich RNA-binding protein contributes to the enhancement of freezing tolerance in Arabidopsis thaliana. Plant J 42:890–900

    Article  CAS  PubMed  Google Scholar 

  • Kim YO, Pan S, Jung CH, Kang H (2007a) A zinc finger-containing glycine-rich RNA-binding protein, atRZ-1a, has a negative impact on seed germination and seedling growth of Arabidopsis thaliana under salt or drought stress conditions. Plant Cell Physiol 48:1170–1181

    Article  CAS  PubMed  Google Scholar 

  • Kim JY, Park SJ, Jang B, Jung CH, Ahn SJ, Goh CH, Cho K, Han O, Kang H (2007b) Functional characterization of a glycine-rich RNA-binding protein 2 in Arabidopsis thaliana under abiotic stress conditions. Plant J 50:439–451

    Article  CAS  PubMed  Google Scholar 

  • Kim JS, Jung HJ, Lee HJ, Kim KA, Goh CH, Woo Y, Oh SH, Han YS, Kang H (2008) Glycine-rich RNA-binding protein 7 affects abiotic stress responses by regulating stomata opening and closing in Arabidopsis thaliana. Plant J 55:455–466

    Article  CAS  PubMed  Google Scholar 

  • Kim WY, Kim JY, Jung HJ, Oh SH, Han YS, Kang H (2010a) Comparative analysis of Arabidopsis zinc finger-containing glycine-rich RNA-binding proteins during cold adaptation. Plant Physiol Biochem 48:866–872

    Article  CAS  PubMed  Google Scholar 

  • Kim JY, Kim WY, Kwak KJ, Oh SH, Han YS, Kang H (2010b) Zinc fingercontaining glycine-rich RNA-binding protein in Oryza sativa has an RNA chaperone activity under cold stress conditions. Plant, Cell Environ 33:759–768

    Article  CAS  Google Scholar 

  • Lee HJ, Kim JS, Yoo SJ, Kang EY, Han SH, Yang KY, Kim YC, McSpadden Gardener B, Kang H (2012) Different roles of glycine-rich RNA-binding protein7 in plant defense against Pectobacterium carotovorum, Botrytis cinerea, and tobacco mosaic viruses. Plant Physiol Biochem 60:46–52

    Article  CAS  PubMed  Google Scholar 

  • Li J, Brader G, Palva ET (2008) Kunitz trypsin inhibitor: an antagonist of cell death triggered by phytopathogens and fumonisin B1 in Arabidopsis. Mol Plant 1:482–495

    Article  CAS  PubMed  Google Scholar 

  • Linthorst HJ, Loon LC, Memelink J, Bol JF (1990) Characterization of cDNA clones for a virus-inducible, glycine-rich protein from petunia. Plant Mol Biol 15:521–523

    Article  CAS  PubMed  Google Scholar 

  • Lorković ZJ (2009) Role of plant RNA-binding proteins in development, stress response and genome organization. Trends Plant Sci 14:229–236

    Article  PubMed  Google Scholar 

  • Molina A, Mena M, Carbonero P, García-Olmedo F (1997) Differential expression of pathogen-responsive genes encoding two types of glycine-rich proteins in barley. Plant Mol Biol 33:803–810

    Article  CAS  PubMed  Google Scholar 

  • Monaghan J, Xu F, Xu S, Zhang Y, Li X (2010) Two putative RNA-binding proteins function with unequal genetic redundancy in the MOS4-associated complex. Plant Physiol 154:1783–1793

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Naqvi SM, Park KS, Yi SY, Lee HW, Bok SH, Choi D (1998) A glycine-rich RNA-binding protein gene is differentially expressed during acute hypersensitive response following Tobacco Mosaic Virus infection in tobacco. Plant Mol Biol 37:571–576

    Article  CAS  PubMed  Google Scholar 

  • Qi Y, Tsuda K, Joe A, Sato M, Nguyen V, Glazebrook J, Alfano JR, Cohen JD, Kataqiri F (2010) A putative RNA-binding protein positively regulates salicylic acid-mediated immunity in Arabidopsis. Mol Plant Microbe Interact 23:1573–1583

    Article  CAS  PubMed  Google Scholar 

  • Sánchez-Rodríguez C, Estévez JM, Llorente F, Hernández-Blanco C, Jordá L, Pagán I, Berrocal M, Marco Y, Somerville S, Molina A (2009) The receptor-like kinase regulates cell wall-mediated resistance to pathogens in Arabidopsis thaliana. Mol Plant Microbe Interact 22:953–963

    Article  PubMed  Google Scholar 

  • Simpson GG, Filipowicz W (1996) Splicing of precursors to mRNA in higher plants: mechanism, regulation and subnuclear organization of the spliceosomal machinery. Plant Mol Biol 32:1–41

    Article  CAS  PubMed  Google Scholar 

  • Staswick PE, Serban B, Rowe M, Tiryaki I, Maldonado MT, Maldonado MC, Suza W (2005) Characterization of an Arabidopsis enzyme family that conjugates amino acids to indole-3-acetic acid. Plant Cell 17:616–627

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Thomma BPHJ, Cammue BPA, Thevissen K (2002) Plant defensins. Planta 216:193–202

    Article  CAS  PubMed  Google Scholar 

  • Villagarcia H, Morin AC, Shpak ED, Khodakovskaya MV (2012) Modification of tomato growth by expression of truncated ERECTA protein from Arabidopsis thaliana. J Exp Bot 63:6493–6504

    Article  CAS  PubMed  Google Scholar 

  • Wang G, Ellendorff U, Kemp B, Mansfield JW, Forsyth A, Mitchell K, Bastas K, Liu C-M, Woods-Tör A, Zipfel C, de Wit PJGM, Jones JDG, Tör M, Thomma BPHJ (2008) A genome-wide functional investigation into the roles of receptor-like proteins in Arabidopsis. Plant Physiol 147:503–517

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Xu T, Han JH, Kang H (2013) Structural features important for the RNA chaperone activity of zinc finger-containing glycine-rich RNA-binding proteins from wheat (Triticum avestivum) and rice (Oryza sativa). Phytochemistry 94:28–35

    Article  CAS  PubMed  Google Scholar 

  • Xu T, Gu L, Choi MJ, Kim RJ, Suh MC, Kang H (2014) Comparative functional analysis of wheat (Triticum aestivum) zinc finger-containing glycine-rich RNA-binding proteins in response to abiotic stresses. PLoS ONE 9:e96877

    Article  PubMed Central  PubMed  Google Scholar 

  • Zhang GY, Feng J, Wu J, Wang XW (2010) BoPMEI1, a pollen-specific pectin methyl esterase inhibitor, has an essential role in pollen tube growth. Planta 231:1323–1334

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by a grant from the Next-Generation BioGreen21 Program (PJ00820303), Rural Development Administration and by a grant from the Mid-career Researcher Program (2011-0017357) through the National Research Foundation of Korea grant funded by the Ministry of Education, Science and Technology, Republic of Korea.

Author information

Authors and Affiliations

  1. College of Life Science, Jiangsu Normal University, Xuzhou, 221116, Jiangsu Province, People’s Republic of China

    Tao Xu

  2. Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, 500-757, Korea

    Tao Xu, Hwa Jung Lee, Nguyen Dinh Sy & Hunseung Kang

Authors
  1. Tao Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hwa Jung Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Nguyen Dinh Sy
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hunseung Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hunseung Kang.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (XLSX 215 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Xu, T., Lee, H.J., Sy, N.D. et al. Wheat (Triticum aestivum) zinc finger-containing glycine-rich RNA-binding protein TaRZ1 affects plant growth and defense response in Arabidopsis thaliana . Plant Growth Regul 76, 243–250 (2015). https://doi.org/10.1007/s10725-014-9994-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10725-014-9994-9

Keywords

Search

Navigation