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Cloning and characterization of GbGI, a DELLA-like gene from cotton (Gossypium barbadense)

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Abstract

The quality of cotton fiber is determined by the fiber development. Many factors affect fiber development of which hormones play an important role. Gibberellic acid (GA) is necessary for fiber elongation in cultured fertilized ovules of cotton (Gossypium barbadense), but the functional mechanism of GA on the promotion of fiber elongation is not completely clear. Our study indicated that the initiation and elongation of fiber are mediated by the application of GA to cultured ovules. Plant responses to GA are mediated by DELLA proteins. We isolated one DELLA-like protein gene, designated GbGI from G. barbadense. GbGI has all the typical motifs of DELLA proteins. Over-expression of GbGI cDNA in Arabidopsis plants resulted in the dwarf phenotype, indicating that GbGI functions as a GA suppressor. Real time RT-PCR analysis showed that GbGI was predominantly expressed in cotton fibers at late developing stage, indicating an important role that GbGI plays in cotton fiber elongation.

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Acknowledgments

We thank Dr. Haichuan Yu for the HPLC analysis of the GA3 content in cotton plant. This work was supported by the National Transgenic Animals and Plants Research Initiative (Grant #2009ZX08005-027B to M.P.), and the Major Technology Project of Hainan (Grant #ZDZX2013023-1).

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Authors and Affiliations

  1. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou, 571101, Hainan, China

    Xiaoling Yu, Mengbin Ruan, Wei Wen, Shuchang Wang & Ming Peng

  2. Agricultural College of Hainan University, Haikou, 570228, Hainan, China

    Xiaoling Yu

  3. College of Life Sciences, Xinjiang Shihezi University, Shihezi, 832003, Xinjiang, China

    Baiming Cui

  4. Department of Plant Biology and Pathology, Rutgers, the State University of New Jersey, New Brunswick, NJ, 08901, USA

    Rong Di

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  1. Xiaoling Yu
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  2. Baiming Cui
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  3. Mengbin Ruan
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  4. Wei Wen
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  5. Shuchang Wang
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  6. Rong Di
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  7. Ming Peng
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Correspondence to Ming Peng.

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Yu, X., Cui, B., Ruan, M. et al. Cloning and characterization of GbGI, a DELLA-like gene from cotton (Gossypium barbadense). Plant Growth Regul 75, 235–244 (2015). https://doi.org/10.1007/s10725-014-9947-3

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  • DOI: https://doi.org/10.1007/s10725-014-9947-3

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