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Isolation and functional analysis of MdCS1: a gene encoding a citrate synthase in Malus domestica (L.) Borkh

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Abstract

Iron (Fe) is one of the essential micronutrients required by all plants. Citric acid (CA) is considered as the chelate substance in the long distance transport of Fe. In the present study, a gene encoding putative citrate synthase (CS) was isolated from Malus domestica (L.) Borkh and designated as MdCS1. The MdCS1 gene encodes a protein of 473 amino acid residues with a theoretical isoelectric point of 7.72 and a predicted molecular mass of 52.7 kDa. Subcellular localization has revealed that MdCS1 is preferentially localized in cytoplasmic membrane. The expression of MdCS1 was enriched in leaf, root, and phloem, which was highly affected by Fe stress and indoleacetic acid treatment, while weakly by abscisic acid treatment in M. domestica seedlings. When MdCS1 was introduced into Arabidopsis, it promoted the synthesis of CS and increased CA content. Overexpression of MdCS1 improved the tolerance to Fe stress in transgenic Arabidopsis, but also led to increased fresh weight, CS activity, and contents of chlorophyll, CA and Fe, especially when dealt with Fe stress.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (31301757), China Postdoctoral Science Foundation (2013M530144), Heilongjiang Postdoctoral Science Foundation (LBH-Z13033), the Doctoral Fund of Northeast Agricultural University (2012RCB09), Scientific Research Fund of Heilongjiang Provincial Education Department (12541004) and Special Research of Public Sector on Agriculture (201103037).

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Authors and Affiliations

  1. College of Horticulture, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Deguo Han, Yan Shi, Zeyuan Yu, Wei Liu, Bingyu Lv, Bing Wang & Guohui Yang

  2. Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of Northeast Region, Ministry of Agriculture, Harbin, 150030, People’s Republic of China

    Deguo Han, Yan Shi, Zeyuan Yu, Bingyu Lv, Bing Wang & Guohui Yang

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  1. Deguo Han
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  2. Yan Shi
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  3. Zeyuan Yu
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  4. Wei Liu
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  6. Bing Wang
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  7. Guohui Yang
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Correspondence to Deguo Han or Guohui Yang.

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Han, D., Shi, Y., Yu, Z. et al. Isolation and functional analysis of MdCS1: a gene encoding a citrate synthase in Malus domestica (L.) Borkh. Plant Growth Regul 75, 209–218 (2015). https://doi.org/10.1007/s10725-014-9945-5

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