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The influence of Si-rich mineral zeolite on the growth processes and adaptive potential of barley plants under cadmium stress

Plant Growth Regulation volume 75, pages 557–565 (2015)Cite this article

Abstract

Hordeum vulgare L. cv. Bartom seedlings grown on a substrate without or with zeolite were exposed to 450 and 1,000 µmol of Cd(NO3)2. Adding the zeolite to a substrate (+Si) resulted in the accumulation of mono- and polysilicic acids in the leaves, an increase in the growth of seedlings and biomass production, a decrease of the contents of thiobarbituric acid reactive substances (TBARs), and an increase in the activities of the antioxidant enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX) and guaiacol peroxidase (GPX). The negative effects of Cd2+, which were reflected in loss of chlorophyll contents and a decrease of the photosynthesis rate, as well as an increase of TBARs contents were observed in the (−Si)-plants to a greater extent than in the (+Si)-plants. The activities of SOD, APX and GPX increased in the barley leaves of (−Si)- and (+Si)-seedlings with an increase of Cd2+ concentration in the acting solution. At the same time the values of the ratios between TBARs concentrations and the activities of SOD, APX and GPX in (−Si)-plants were higher than that in (+Si)-plants both under optimal conditions and with Cd stress. This suggests that the use of Si-rich mineral zeolite for growing barley stimulates metabolism and increases the resistance of plants to cadmium stress through a shift in the dynamic equilibrium between the rate of oxidative destruction and the activity of antioxidant systems in favor of the latter.

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Abbreviations

APX:

Ascorbate peroxidase

FW:

Fresh weight

GPX:

Guaiacol peroxidase

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

TBARs:

Thiobarbituric acid reactive substances

References

  • Al-Busaidi A, Yamamoto T, Inoue M, Egrinya EE, Mori Y, Irshad M (2008) Effects of zeolite on soil nutrients and growth of barley following irrigation with saline water. In: The 3rd international conference on water resources and arid environments and the 1st Arab Water Forum, pp 1–11

  • Alcantara E, Romera FJ, Canete M, De La Guardia MD (1994) Effects of heavy metals on both induction and function of root Fe(III) reductase in Fe-deficient cucumber (Cucumis sativus L.) plants. J Exp Bot 45:1893–1898

    Article  CAS  Google Scholar 

  • Ali S, Farooq MA, Yasmeen T, Hussain S, Arif MS, Abbas F, Bharwana SA, Zhang G (2013) The influence of silicon on barley growth, photosynthesis and ultra-structure under chromium stress. Ecotoxicol Environ Saf 89:66–72

    Article  CAS  PubMed  Google Scholar 

  • Asada K (2006) Production and scavenging of reactive oxygen species in chloroplasts and their functions. Plant Physiol 141:391–396

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Azevedo RA, Gratão PL, Monteiro CC, Carvalho R (2012) What is new in the research on cadmium-induced stress in plants? Food Energy Secur 1(2):133–140

    Article  Google Scholar 

  • Balakhnina T, Borkowska A (2013) Effects of silicon on plant resistance to environmental stresses: review. Int Agrophys 27:225–232

    Article  CAS  Google Scholar 

  • Balakhnina TI, Kosobryukhov AA, Ivanov AA, Kreslavskii VD (2005) The effect of cadmium on CO2 exchange, variable fluorescence of chlorophyll, and the level of antioxidant enzymes in pea leaves. Russian J of Plant Physiol, 52(1):15–20 (Translated from Fiziologiya Rastenii (2005) 52(1):21–26)

  • Balakhnina TI, Gavrilov AB, Wlodarczyk TM, Borkowska A, Nosalewicz M, Fomina IR (2009) Dihydroquercetin protects barley seeds against mould and increases seedling adaptive potential under soil flooding. Plant Growth Regul 57:127–135

  • Balakhnina TI, Matichenkov VV, Włodarczyk T, Borkowska A, Nosalewicz M, Fomina IR (2012) Effects of silicon on growth processes and adaptive potential of barley plants under optimal soil watering and flooding. Plant Growth Regul 67:35–43

    Article  CAS  Google Scholar 

  • Baraboi VA (1991) Stress mechanisms and lipid peroxidation. Usp Sovrem Biol 111(6):923–932

    CAS  Google Scholar 

  • Belanger RR (2005) The role silicon in plant–pathogen interaction: toward universal model. In: Korndorfer GH (ed) Proceedings of the 3rd silicon agricultural conference. Universidad Federal de Uberlandia, Uberlandia, pp 34–40

    Google Scholar 

  • Biel KY, Matichenkov VV, Fomina IR (2008) Protective role of silicon in living systems. In: Martirosyan DM (ed) Functional foods for chronic diseases. D&A Inc., Richardson, pp 208–231

    Google Scholar 

  • Das P, Samantaray S, Rout GR (1997) Studies on cadmium toxicity in plants: a review. Environ Pollut 98:29–36

    Article  CAS  PubMed  Google Scholar 

  • Duncan DB (1955) Multiple range and multiple F tests. Biometrics 11:1–42

    Article  Google Scholar 

  • Epstein E (1999) Silicon. Ann Rev Plant Physiol Plant Mol Biol 50:641–664

    Article  CAS  Google Scholar 

  • Eshghi S, Mahmoodabadi MR, Abdi GR, Jamali B (2010) Zeolite ameliorates the adverse effect of cadmium contamination on growth and nodulation of soybean plant (Glycine max L.). J Biol Environ Sci 4(10):43–50

    Google Scholar 

  • Gavrilenko VF, Ladygina ME, Handobina LM (1975) Opredelenie peroksidaznoi funktsii: Metod Boyarkina (Evaluation of the peroxidase function: the method of Boyarkin). In: Rubin BA (ed) Bol’shoi praktikum po fiziologii rastenii (Big practical work in plant physiology). Visshaya Shkola, Moscow, pp 284–286

    Google Scholar 

  • Giannopolitis CN, Ries SK (1977) Superoxide dismutases. I. Occurrence in higher plants. Plant Physiol 59:309–314

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Gong H, Zhu X, Chen K, Wang S, Zhang C (2005) Silicon alleviates oxidative damage of wheat plants in pots under drought. Plant Sci 169:313–321

    Article  CAS  Google Scholar 

  • Gong HJ, Chen KM, Zhao ZG, Chen GC, Zhou WJ (2008) Effects of silicon on defense of wheat against oxidative stress under drought at different developmental stages. Biol Plant 52:592–596

    Article  CAS  Google Scholar 

  • Grataö PL, Polle A, Lea PJ, Azevedo RA (2005) Making the life of heavy metals-stressed plant a little easier. Funct Plant Biol 32(6):481–494

    Article  Google Scholar 

  • Gunes A, Inal A, Bagci EG, Coban S, Sahin O (2007) Silicon increases boron tolerance and reduces oxidative damage of wheat grown in soil with excess boron. Biol Plant 51:571–574

    Article  CAS  Google Scholar 

  • Hattori T, Inanaga S, Araki H, An P, Morita S, Luxova M, Lux A (2005) Application of silicon enhanced drought tolerance in Sorghum bicolor. Physiol Plant 123:459–466

    Article  CAS  Google Scholar 

  • Iler RK (1979) The chemistry of silica. Wiley Press, New York, p 866

    Google Scholar 

  • Kabata-Pendias A (2011) Trace elements in soils and plants, 4th edn. CRC Press, Boca Raton, pp 287–288

    Google Scholar 

  • Liang Y, Chen Q, Liu Q, Zhang W, Ding R (2003) Exogenous silicon (Si) increases antioxidant enzyme activity and reduces lipid peroxidation in roots of salt-stressed barley (Hordeum vulgare L.). J Plant Physiol 160:1157–1164

    Article  CAS  PubMed  Google Scholar 

  • Liang Y, Sun W, Zhu YG, Christie P (2007) Mechanisms of silicon mediated alleviation of abiotic stresses in higher plants. Environ Pollut 147:422–428

    Article  CAS  PubMed  Google Scholar 

  • Lichtenthaler HK, Wellburn AR (1985) Determination of total carotenoids and chlorophylls A and B of leaf in different solvents. Biol Soc Trans 11:591–592

    Google Scholar 

  • Ma JF (2003) Function of silicon in higher plants. In: Muller WEG (ed) Progress in molecular and sub cellular biology. Springer, Berlin, pp 127–147

    Google Scholar 

  • Ma JF (2004) Role of silicon in enhancing the resistance of plants to biotic and abiotic stresses. Soil Sci Plant Nutr 50:11–18

    Article  CAS  Google Scholar 

  • Ma JF, Takahashi E (2002) Soil, fertilizer, and plant silicon research in Japan. Elsevier, Amsterdam, p 275

    Google Scholar 

  • Matichenkov VV, Kosobryukhov AA (2004) Si effect on the plant resistance to salt toxicity. In: Proceedings of the 13th international soil conservation organization conference (ISCO), Brisbane, pp 287–295

  • Matichenkov VV, Calvert DV, Snyder GH (2000) Prospective silicon fertilization for citrus in Florida. Proc Soil Crop Sci Florida 59:137–141

    Google Scholar 

  • Matoh T, Murata S, Takahashi E (1991) Effect of silicate application on photosynthesis of rice plants. Jpn J Soil Sci Plant Nutr 62:248–252

    CAS  Google Scholar 

  • Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410

    Article  CAS  PubMed  Google Scholar 

  • Nagajyoti PC, Lee KD, Sreekanth TVM (2010) Heavy metals, occurrence and toxicity for plants: a review. Environ Chem Lett 8:199–216

    Article  CAS  Google Scholar 

  • Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880

    CAS  Google Scholar 

  • Peter A, Mihaly-Cozmuta L, Mihaly-Cozmuta A, Nicula C (2011) The role of natural zeolite and of zeolite modified with ammonium ions to reduce the uptake of lead, zinc, copper and iron ions in Hieracium aurantium and Rumex acetosella grown on tailing ponds. Analele Universităţii din Oradea—Fascicule Biologie 18(2):128–135

    Google Scholar 

  • Smiri M (2011) Effect of cadmium on germination, growth, redox and oxidative properties in Pisum sativum seeds. J Environ Chem Ecotoxicol 3(3):52–59

    CAS  Google Scholar 

  • Uchiyama M, Mihara M (1978) Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem 86:287–297

    Article  Google Scholar 

  • Wagner GJ (1993) Accumulation of cadmium in crop plants and its consequences to human health. Adv Agron 51(1):173–212

    Article  CAS  Google Scholar 

  • We J-F, Deng M-H, Gong M (2012) Cd2+ stress induces two waves of H2O2 accumulation associated with ROS-generating system and ROS-scavenging system in cultured tobacco cells. Aust J Crop Sci 6(5):846–853

    Google Scholar 

  • Zhu Z, Wei G, Li J, Qian Q, Yu J (2004) Silicon alleviates salt stress and increases antioxidant enzymes activity in leaves of salt stressed cucumber (Cucumis sativus L.). Plant Sci 167:527–553

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Area, 142290, Russia

    Tamara I. Balakhnina, Vladimir V. Matichenkov & Anatoly A. Kosobryukhov

  2. Institute of Agrophysics, Polish Academy of Sciences, 20-290, Lublin, Poland

    Piotr Bulak & Teresa M. Włodarczyk

Authors
  1. Tamara I. Balakhnina
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  2. Piotr Bulak
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  3. Vladimir V. Matichenkov
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  4. Anatoly A. Kosobryukhov
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  5. Teresa M. Włodarczyk
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Correspondence to Tamara I. Balakhnina.

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Balakhnina, T.I., Bulak, P., Matichenkov, V.V. et al. The influence of Si-rich mineral zeolite on the growth processes and adaptive potential of barley plants under cadmium stress. Plant Growth Regul 75, 557–565 (2015). https://doi.org/10.1007/s10725-014-0021-y

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  • DOI: https://doi.org/10.1007/s10725-014-0021-y

Keywords

  • Antioxidant enzymes
  • Cadmium
  • Oxidative destruction
  • Photosynthesis
  • Silicon
  • Zeolite