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Physiological response to heat stress of tomato ‘Micro-Tom’ plants expressing high and low levels of mitochondrial sHSP23.6 protein

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Abstract

Tomato ‘Micro-Tom’ plants were transformed for high or low expression of the mitochondrial small “heat shock” protein (HSP) (MT-sHSP23.6) to evaluate their response to high temperature. The plants were raised for 59 days under a controlled temperature, photoperiod and photon flow density and then subjected to heat stress for 24 h at 37 °C, followed by a recovery period under normal conditions (21 ± 2 °C). The cycle was repeated. The chlorophyll a fluorescence intensity was measured, and the parameters of the JIP-test were calculated. The gas exchange was also evaluated. The JIP-test showed significantly different responses of the genotypes to heat stress. The parameters of photosystem I activity and the net assimilation of CO2 increased during the first stress cycle in genotypes with a high expression of MT-sHSP23.6 and in non-transformed plants; however, the net assimilation of CO2 decreased in genotypes with a low expression of MT-sHSP23.6. The data suggest that MT-sHSP23.6 participates in the heat tolerance mechanism, considering that the suppression of this protein resulted in greater physiological damage during heat stress.

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Abbreviations

ABS/RC:

Absorption flux (of antenna Chls) per RC

AS:

Plants transformed in the anti-sense orientation

DI0/RC:

Dissipation flux per RC

ET0/RC:

Electron transport flux (further than QA−) per RC

MT-sHSP:

Mitochondrial small HSP

N:

Turnover number as reduction, oxidation, re-reduction of QA in time span from light until reaching FM

OEC:

Oxygen evolution complex

PIABS :

Performance index (potential) for energy conservation from exciton to the reduction of intersystem electron acceptors

PItotal :

Performance index (potential) for energy conservation from exciton to the reduction of PSI end acceptors

PSII:

Photosystem II

PSI:

Photosystem I

RC:

Reaction centre

RE0/RC:

Electron flux reducing end electron acceptors at the PSI acceptor side, per RC

sHSPs:

Small HSPs

S:

Plants transformed in the sense orientation

Sm :

Total complementary area normalised above the transient OJIP

Ss :

Total complementary area normalised above the curve of only transient OJ

TR0/RC:

Trapped energy flux (leading to QA reduction) per RC

WT:

Non-transformed plants

φPo :

Maximum quantum yield for primary photochemistry

φEo :

Quantum yield for electron transport (ET)

φRo :

Quantum yield for reduction of end electron acceptors at the PSI acceptor side (RE)

φDo :

Quantum yield for dissipation (DI)

Ψ0 :

Probability (at time 0) that a trapped exciton moves an electron into the electron transport chain beyond QA−

δ0 :

Probability with which an electron from the intersystem electron carriers moves to reduce end electron acceptors at the PSI acceptor side

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Acknowledgments

We acknowledge the financial support of the CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), Ministério de Ciência e Tecnologia, FINEP, CAPES (Coordenação de Aperfeiçoamento de pessoal de Nível Superior), and FAPERGS (Fundação de Amparo à Pesquisa do Estudo do Rio Grande do Sul).

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Authors and Affiliations

  1. Departamento de Botânica, Instituto de Biologia, Universidade Federal de Pelotas, Campus Capão do Leão, Pelotas, RS, 96010-900, Brazil

    Cristina Moll Huther, Aline Ramm & Marcos Antonio Bacarin

  2. Departamento de Ciência e Tecnologia Agroindustrial, Faculdade de Agronomia Eliseo Maciel, Universidade Federal de Pelotas, Campus Capão do Leão, Pelotas, RS, 96010-900, Brazil

    Cesar Valmor Rombaldi

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  1. Cristina Moll Huther
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  2. Aline Ramm
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  3. Cesar Valmor Rombaldi
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  4. Marcos Antonio Bacarin
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Correspondence to Marcos Antonio Bacarin.

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Huther, C.M., Ramm, A., Rombaldi, C.V. et al. Physiological response to heat stress of tomato ‘Micro-Tom’ plants expressing high and low levels of mitochondrial sHSP23.6 protein. Plant Growth Regul 70, 175–185 (2013). https://doi.org/10.1007/s10725-013-9790-y

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