Abstract
In Trifolium subterraneum, oxidative stress caused by ozone has been shown to result in more severe visible foliar injuries when plants were kept in dim broadband white light during the night (i.e. a long photoperiod) compared to darkness during the night (a short photoperiod). As phytochrome signalling is involved in photoperiod sensing, the effect of night-time red and far-red illumination on the ozone-induced response was studied. T. subterraneum plants were treated with ozone enriched air (70 ppb) for either 1 h for a single day or 6 h for three consecutive days. After the first ozone exposure, plants were separated into six night-time light regimes during the two subsequent nights (10 h day, 14 h night): (1) darkness, (2) far-red light (FR), (3) a short night-break of red followed by far-red light during an otherwise dark night (R FR), (4) a short night-break of red, far-red and finally red light during an otherwise dark night (R FR R), (5) dim white light (L) and (6) red light (R). The treatments L and R resulted in significantly more severe ozone-induced visible foliar injuries relative to D and FR treatments, indicating a phytochrome-mediated response. The night-breaks resulted in a photoreversible and significantly different ozone response depending on the light quality of the last light interval (R FR or R FR R), supporting a photoreversible (between Pr and Pfr) phytochrome signalling response. Thus, in T. subterraneum, the outcome of oxidative stress due to ozone appears to depend on the photoperiod mediated by the night-time conformation of phytochrome.
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Acknowledgments
We thank Associate Professor Sissel Torre, Department of Plant and Environmental Sciences, Norwegian University of Life Science, for lending us the R and FR emitting diodes, Bjørn Johnsen at the Norwegian Radiation Protection Authority for letting us use their Bentham spectroradiometer to characterize the light sources and the personnel at the Phytotron, University of Oslo for technical assistance.
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Eriksen, A.B., Vollsnes, A.V., Futsaether, C.M. et al. Reversible phytochrome regulation influenced the severity of ozone-induced visible foliar injuries in Trifolium subterraneum L.. Plant Growth Regul 68, 517–523 (2012). https://doi.org/10.1007/s10725-012-9729-8
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DOI: https://doi.org/10.1007/s10725-012-9729-8