Plant Growth Regulation

, Volume 68, Issue 2, pp 293–301 | Cite as

Effect of plant growth regulators on plant regeneration of Dioscorea remotiflora (Kunth) through nodal explants

  • A. Bernabé-Antonio
  • F. Santacruz-Ruvalcaba
  • F. Cruz-Sosa
Original paper

Abstract

Dioscorea remotiflora (Kunth) is an important wild plant that produces tuberous roots used as a source of food in the Western part of Mexico. Lack of planting material and inefficiency of traditional methods of propagation are the main constraints for implementing large-scale cultivation. In contrast, tissue culture techniques allow increasing multiplication and rapid production of plant material. In this regard, leaves or nodal segments were incubated on MS, B5 and WPM culture media with different PGRs in order to obtain an efficient micropropagation protocol. Leaves explants were unable to inducing shoots or callus. However, nodal segments produced axillary shoots and/or callus in all culture media. MS containing 2.33 μM KIN was the most suitable to inducing shoots; an average of 6.6 shoots per segment for 100 % explants was obtained, which displayed also the greater number of nodes (5.0) and leaves (7.9) per segment. A decrease on shoot proliferation was observed combining BA or KIN with 2,4-D or NAA. However, small brownish callus were induced on 100 % of segments using 2.33 μM KIN with 5.37 μM 2,4-D or 9.30 μM KIN plus 2.69 μM NAA. In contrast, by adding 2.69 μM NAA, 66.4 % of the nodal segments formed shoots and produced also yellowish friable callus on the base of the shoots. Shoots were easily rooted with 8.28 μM IBA (96.9 %), displaying the greatest root and shoot biomass, but maximum number of tuberous roots, and root or tuberous root biomass was produced increasing IBA (20.7 μM).

Keywords

Tissue culture Culture media Micropropagation Dry weight Tuberous roots 

Abbreviations

ANOVA

Analysis of variance

BA

N6-Benzyladenine

DW

Dry weight

IBA

Indole-3-butyric acid

PGRs

Plant growth regulators

PVP

Polyvinylpyrrolidone

KIN

Kinetin

NAA

α-Naphthaleneacetic acid

WPM

Woody plant medium

2,4-D

2,4-Dichlorophenoxyacetic acid

References

  1. Alizadeh S, Mantell SH, Viana AM (1998) In vitro shoot culture and microtuber induction in the steroid yam Dioscorea composita hemsl. Plant Cell Tiss Org Cult 53:107–112CrossRefGoogle Scholar
  2. Ayensu ES (1972) Anatomy of the monocotyledons VI dioscoreales. Oxford Press, OxfordGoogle Scholar
  3. Behera KK, Sahoo S, Prusti A (2009) Regeneration of plantlet of water yam (Dioscorea oppositifolia L.) through in vitro culture from nodal explant. Not Bot Horti Agrobot Cluj-Na 37:94–102Google Scholar
  4. Borges M, Ceiro W, Meneses S, Aguilera N, Vazquez J, Infante Z, Fonseca M (2004) Regeneration and multiplication of Dioscorea alata germplasm maintained in vitro. Plant Cell Tiss Org Cult 76:87–90CrossRefGoogle Scholar
  5. Chen Y, Fan J, Yi F, Luo Z, Fu Y (2003) Rapid clonal propagation of Dioscorea zingiberensis. Plant Cell Tiss Org Cult 73:75–80CrossRefGoogle Scholar
  6. Chen FQ, Fu Y, Wang DL, Gao X, Wang L (2007) The effect of plant growth regulators and sucrose on the micropropagation and microtuberization of Dioscorea nipponica makino. J Plant Growth Regul 26:38–45CrossRefGoogle Scholar
  7. Forsyth C, Staden VJ (1982) An improved method of in vitro propagation of Dioscorea bulbifera. Plant Cell Tiss Org Cult 1:275–281CrossRefGoogle Scholar
  8. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158PubMedCrossRefGoogle Scholar
  9. George EF, Hall MA, Klerk GJ (2008) Plant propagation by tissue culture. Springer, NewYorkGoogle Scholar
  10. González CM (1984) Especies Vegetales de Importancia Económica en México. Contribución a su conocimiento, Porrúa, México, D.F.Google Scholar
  11. Guízar-Miranda A, Montañéz-Soto JL, García-Ruiza I (2008) Parcial caracterización de nuevos almidones obtenidos del tubérculo de camote de cerro (Dioscorea spp). Rev Iber Tecnología Postcosecha 9:81–88Google Scholar
  12. Huang XL, Yang Bo HuCG, Yao JL (2009) In vitro induction of inflorescence in Dioscorea zingiberensis. Plant Cell Tiss Org Cult 99:209–215CrossRefGoogle Scholar
  13. Islam MT, Keller ERJ, Dembele DP (2008) Effects of growth regulators on in vitro propagation and tuberization of four Dioscorea species. Plant Tissue Cult Biotech 18:25–35Google Scholar
  14. Kohmura H, Araki H, Imoto M (1995) Micropropagation of ‘Yamatoimo’ Chinese yam (Dioscorea opposita) from immature leaves. Plant Cell Tiss Org Cult 40:271–276CrossRefGoogle Scholar
  15. Lloyd G, McCown B (1980) Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture. Comb Proc Int Plant Prop Soc 30:421–427Google Scholar
  16. Mahesh R, Muthuchelian K, Maridass M, Raju G (2010) In vitro propagation of wild yam, Dioscorea wightii through nodal cultures. Int J Biol Technol 1:111–113Google Scholar
  17. Martine J, Cappadocia M (1992) Effects of some growth regulators on in vitro tuberization in Dioscorea alata L. ‘Brazo fuerte’ and D. abyssinica Hoch. Plant Cell Rep 11:34–38Google Scholar
  18. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–479CrossRefGoogle Scholar
  19. Ondo OP, Kevers C, Dommes J (2007) Axillary proliferation and tuberisation of Dioscorea cayenensisD. rotundata complex. Plant Cell Tissues Organ Cult 91:107–111CrossRefGoogle Scholar
  20. Ondo OP, Kevers C, Dommes J (2010) Tuber formation and growth of Dioscorea cayenensisD. rotundata complex: interactions between exogenous and endogenous jasmonic acid and polyamines. Plant Growth Regul 60:247–253CrossRefGoogle Scholar
  21. Phillips GC, Collins GB (1979) In vitro tissue culture of selected legumes and plant regeneration from callus cultures of red clover. Crop Sci 19:59–64CrossRefGoogle Scholar
  22. Pierik RLM (1987) In vitro culture of higher plants. Wageningen, The NetherlandsCrossRefGoogle Scholar
  23. Poornima GN, Ravishankar RV (2007) In vitro propagation of wild yams, Dioscorea oppositifolia (Linn) and Dioscorea pentaphylla (Linn). Afr J Biotechnol 6:2348–2352Google Scholar
  24. Sakakibara H (2010) Cytokinin biosynthesis and metabolism. In: Davies PJ (ed) Plant hormones. Cornell University, New York, pp 95–114CrossRefGoogle Scholar
  25. Staba EJ (1982) Plant tissue culture as a source of biochemicals. CRC Press, Boca RatonGoogle Scholar
  26. Vaillant V, Bade P, Constant C (2005) Photoperiod affects the growth and development of yam plantlets obtained by in vitro propagation. Biol Plant 49:355–359CrossRefGoogle Scholar
  27. Viana AM, Mantell SH (1989) Callus induction and plant regeneration from excised zygotic embryos of the seed-propagated yams Dioscorea composite Hemsi. and D. cayenensis Lam. Plant Cell Tiss Org Cult 16:113–122CrossRefGoogle Scholar
  28. Xu J, Yin H, Wang W, Mi Q, Liu X (2009) Effects of sodium nitroprusside on callus induction and shoot regeneration in micropropagated Dioscorea opposita. Plant Growth Regul 59:279–285CrossRefGoogle Scholar
  29. Yan H, Yang L, Li Y (2011) Axillary shoot proliferation and tuberization of Dioscorea fordii Prain et Burk. Plant Cell Tiss Org Cult 104:193–198CrossRefGoogle Scholar
  30. Yuan S, Yan YC, Lin HH (2005) Plant regeneration through somatic embryogenesis from callus cultures of Dioscorea zingiberensis. Plant Cell Tiss Org Cult 80:157–161CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • A. Bernabé-Antonio
    • 1
  • F. Santacruz-Ruvalcaba
    • 1
  • F. Cruz-Sosa
    • 2
  1. 1.Department of Agricultural ProductionUniversity Center of Biological and Agricultural Sciences, University of GuadalajaraZapopanMexico
  2. 2.Department of Biotechnology, Division of Biological and Health SciencesMetropolitan Autonomous University-Iztapalapa CampusMexico D.FMexico

Personalised recommendations