Comparative mineral and hormonal analyses of wild type and TLS somaclonal variant derived from oil palm (Elaeis guineensis Jacq. var. tenera) tissue culture
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Comparative mineral and hormonal analyses were made on tissue culture derived truncated leaf syndrome and wild type oil palm seedlings. Mineral analysis confirmed that Boron, Zinc and chlorophyll levels were significantly lower in truncated leaf syndrome leaves than those of wild type. Hormonal analysis also revealed various cytokinin derivatives such as trans-zeatin, trans-zeatin riboside, trans-zeatin O-glucoside and trans-zeatin riboside 5′mono phosphate were significantly higher in truncated leaf syndrome leaves compared to wild type leaves. Brassinolide level was also significantly higher in truncated leaf syndrome leaves than those of the wild type. These observations suggest that the truncated leaf syndrome abnormality could be associated to high cytokinin and brassinosteroid production which affects the uptake of Boron and Zinc.
KeywordsTruncated leaf syndrome Boron Zinc Cytokinin Brassinosteroid Brassinolide Oil palm Abnormality
The authors thank TWOWS (Third World Organization for Women in Sciences) for the postgraduate research fellowship to Ms. Habib S.H., the Ministry of Higher Education, Malaysia for funding this project through the FRGS grant (05-10-07-364FR), FELDA Agricultural Services Sdn. Bhd. for providing the oil palm seedlings and Czech Ministry of Education, grant No. ED0007/01/01 Centre of the Region Haná for Biotechnological and Agricultural Research for financial support.
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