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Plant Growth Regulation

, Volume 67, Issue 3, pp 257–270 | Cite as

Direct induction of protocorm-like bodies from shoot tips, plantlet formation, and clonal fidelity analysis in Anthurium andreanum cv. CanCan

  • Saikat GantaitEmail author
  • Uma Rani Sinniah
  • Nirmal Mandal
  • Prakash Kanti Das
Original paper

Abstract

Protocorm-like bodies (PLBs) were induced directly at high frequency from wounded surface of Anthurium andreanum cv. CanCan shoot tip-ends, used as explants. In order to obtain PLB directly, the influence of different types and concentrations of cytokinins were evaluated. Amid the cytokinins, N6-(∆2-isopentenyl)-adenine (2-iP) at a concentration of 15 μM was most effective in inducing PLB whereby ~98 (97.8) % of explants induced PLB with an average of 120 PLBs per shoot tip within 50 days of culture. Stereomicroscopic observation meticulously revealed the sequential changes from initiation to maturation of PLB gradually forming shoot apical meristem, shoot primordia and leaf primordia. Mature PLBs showed significant shoot proliferation (98.4 %) in media containing 10 μM 6-furfurylaminopurine forming 17 shoots per PLB within 30 days. The inclusion of activated charcoal (AC) in media containing auxin had promotive effect on rooting whereby 5 μM indole-3-butyric acid plus 500 μM AC resulted in highest number and length of roots. Successfully acclimatized plants, subjected to random amplified polymorphic DNA assessment for genetic fidelity, did not show any variation. Thus, this complete study has successfully outlined a rapid, high frequency direct induction of PLB of Anthurium from shoot tips inclusive of shoot proliferation, rooting and acclimatization.

Keywords

Conversion Cytokinins Organogenesis Plant growth regulators PLB RAPD Scanning electron microscope 

Abbreviations

2-iP

N6-(∆2-isopentenyl)-adenine

AC

Activated charcoal

ADS

Adenine sulphate

BA

N6-benzyladenine

IAA

Indole-3-acetic acid

IBA

Indole-3-butyric acid

MS

Murashige and Skoog (1962)

Kn

Kinetin or 6-furfurylaminopurine

PGRs

Plant growth regulators

PLBs

Protocorm-like bodies

VPSEM

Variable pressure scanning electron microscope

TDZ

Thidiazuron or N-phenyl-N -(1,2,3-thidiazol-5-yl)urea

MT

Meristematic tissue

SAM

Shoot apical meristem

LP

Leaf primordia

SP

Shoot primordia

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Saikat Gantait
    • 1
    • 2
    Email author
  • Uma Rani Sinniah
    • 1
  • Nirmal Mandal
    • 2
  • Prakash Kanti Das
    • 3
  1. 1.Department of Crop Science, Faculty of AgricultureUniversiti Putra MalaysiaSerdang, SelangorMalaysia
  2. 2.Department of Biotechnology, Instrumentation and Environmental ScienceBidhan Chandra Krishi ViswavidyalayaMohanpurIndia
  3. 3.Department of GeneticsBidhan Chandra Krishi ViswavidyalayaMohanpurIndia

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