Plant Growth Regulation

, Volume 67, Issue 3, pp 257–270 | Cite as

Direct induction of protocorm-like bodies from shoot tips, plantlet formation, and clonal fidelity analysis in Anthurium andreanum cv. CanCan

  • Saikat GantaitEmail author
  • Uma Rani Sinniah
  • Nirmal Mandal
  • Prakash Kanti Das
Original paper


Protocorm-like bodies (PLBs) were induced directly at high frequency from wounded surface of Anthurium andreanum cv. CanCan shoot tip-ends, used as explants. In order to obtain PLB directly, the influence of different types and concentrations of cytokinins were evaluated. Amid the cytokinins, N6-(∆2-isopentenyl)-adenine (2-iP) at a concentration of 15 μM was most effective in inducing PLB whereby ~98 (97.8) % of explants induced PLB with an average of 120 PLBs per shoot tip within 50 days of culture. Stereomicroscopic observation meticulously revealed the sequential changes from initiation to maturation of PLB gradually forming shoot apical meristem, shoot primordia and leaf primordia. Mature PLBs showed significant shoot proliferation (98.4 %) in media containing 10 μM 6-furfurylaminopurine forming 17 shoots per PLB within 30 days. The inclusion of activated charcoal (AC) in media containing auxin had promotive effect on rooting whereby 5 μM indole-3-butyric acid plus 500 μM AC resulted in highest number and length of roots. Successfully acclimatized plants, subjected to random amplified polymorphic DNA assessment for genetic fidelity, did not show any variation. Thus, this complete study has successfully outlined a rapid, high frequency direct induction of PLB of Anthurium from shoot tips inclusive of shoot proliferation, rooting and acclimatization.


Conversion Cytokinins Organogenesis Plant growth regulators PLB RAPD Scanning electron microscope 





Activated charcoal


Adenine sulphate




Indole-3-acetic acid


Indole-3-butyric acid


Murashige and Skoog (1962)


Kinetin or 6-furfurylaminopurine


Plant growth regulators


Protocorm-like bodies


Variable pressure scanning electron microscope


Thidiazuron or N-phenyl-N -(1,2,3-thidiazol-5-yl)urea


Meristematic tissue


Shoot apical meristem


Leaf primordia


Shoot primordia


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Saikat Gantait
    • 1
    • 2
    Email author
  • Uma Rani Sinniah
    • 1
  • Nirmal Mandal
    • 2
  • Prakash Kanti Das
    • 3
  1. 1.Department of Crop Science, Faculty of AgricultureUniversiti Putra MalaysiaSerdang, SelangorMalaysia
  2. 2.Department of Biotechnology, Instrumentation and Environmental ScienceBidhan Chandra Krishi ViswavidyalayaMohanpurIndia
  3. 3.Department of GeneticsBidhan Chandra Krishi ViswavidyalayaMohanpurIndia

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