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Ameliorative effects of squash (Cucurbita moschata Duchesne ex Poiret) leaf extracts on oxidative stress

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Abstract

This study screened paraquat-tolerant plants among 10 plant species, including monocots and dicots angiosperms. Squash (Cucurbita moschata Duchesne ex Poiret) and kidney bean (Phaseolus vulgaris L.) plants exhibited the highest photooxidation-tolerant phenotypes upon a foliar treatment with paraquat. A foliar treatment with paraquat pre-mixed with leaf water extracts from the squash plant significantly alleviated paraquat-induced oxidative damage in maize, but this was not the case after a treatment with the hydrophobic phase of the leaf extracts. In particular, the water extract from young leaves (4th true leaf) of squash plants conferred tenfold higher tolerance to oxidative damage in paraquat-treated leave tissues compared to paraquat-only treatment. This tolerance was tightly linked not only to the increased amounts of ascorbic acid and dehydroascorbate antioxidants in the damaged leaves, but also to the reduced chlorophyll loss, lipid peroxidation, and cellular electrolyte leakage. Moreover, the protective effects of the water extract were apparent when using another bipyridyl herbicide, diquat, but not with a diphenyl-ether herbicide, oxyfluorfen. On the other hand, pre-treatment with the extract prior to the onset of drought or cold stress had no significant antioxidative effect on the treated tissues.

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Abbreviations

PQ:

Paraquat

MDA:

Malondialdehyde

WE:

Water extract

DW:

Distilled water

BDW:

Boiled distilled water

DAT:

Days after treatment

DHA:

Dehydroascorbate

AsA:

Ascorbic acid

ROS:

Reactive oxygen species

References

  • Aono M, Saji H, Sakamoto A, Tanaka K, Kondo N, Tanaka K (1995) Paraquat tolerance of transgenic Nicotiana tabacum with enhanced activities of glutathione reductase and superoxide dismutase. Plant Cell Physiol 36:1687–1691

    PubMed  CAS  Google Scholar 

  • Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–399

    Article  PubMed  CAS  Google Scholar 

  • Asada K, Takahashi M (1987) Photoinhibition. Elsevier, Amsterdam, pp 227–287

    Google Scholar 

  • Buege JA, Aust SD (1978) Microsomal lipid peroxidation. Methods Enzymol 52:302–310

    Google Scholar 

  • Conklin PL, Williams EH, Last RL (1996) Environmental stress sensitivity of an ascorbic acid-deficient Arabidopsis mutant. Proc Natl Acad Sci USA 93:9970–9974

    Article  PubMed  CAS  Google Scholar 

  • Creissen G, Firmin J, Fryer M, Kular B, Leyland N, Reynolds H, Pastori G, Wellburn F, Baker N, Wellburn A, Mullineaux P (1999) Elevated glutathione biosynthetic capacity in the chloroplasts of transgenic tobacco plants paradoxically causes increased oxidative stress. Plant Cell 11:1277–1292

    Article  PubMed  CAS  Google Scholar 

  • Dhindsa RA, Plumb-Dhindsa P, Thorpe TA (1981) Leaf senescence: correlated with increased permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J Exp Bot 32:93–101

    Article  CAS  Google Scholar 

  • Dodge AD (1975) Some mechanisms of herbicide action, vol 62. Science Progress, Oxford, pp 447–466

  • Du Z, Bramlage WJ (1992) Modified thiobarbituric acid assay for measuring lipid oxidation in sugar-rich plant tissue extracts. J Agric Food Chem 40:1566–1570

    Article  CAS  Google Scholar 

  • Faize M, Burgos L, Faize L, Piqueras A, Nicolas E, Barba-Espin G, Clemente-Moreno MJ, Alcobendas R, Artlip T, Hernandez JA (2011) Involvement of cytosolic ascorbate peroxidase and Cu/Zn-superoxide dismutase for improved tolerance against drought stress. J Exp Bot 62:2599–2613

    Article  PubMed  CAS  Google Scholar 

  • Funderburk HH Jr, Lawrence JM (1964) Mode of action and metabolism of diquat and paraquat. Weeds 12:259–264

    Article  CAS  Google Scholar 

  • Hiscox JD, Israelstam GF (1979) A method for the extraction of chlorophyll from leaf tissue without maceration. Can J Bot 57:1332–1334

    Article  CAS  Google Scholar 

  • Liu Z-J, Zhang X-L, Bai J-G, Suo B-X, Xu P-L, Wang L (2009) Exogenous paraquat changes antioxidant enzyme activities and lipid peroxidation in drought-stressed cucumber leaves. Sci Hortic 121:138–143

    Article  CAS  Google Scholar 

  • Mascher R, Fischer S, Scheiding W, Neagoe A, Bergmann H (2005) Exogenous 2-aminoethanol can diminish paraquat induced oxidative stress in barley (Hordeum vulgare L.). Plant Growth Regul 45:103–112

    Article  CAS  Google Scholar 

  • Pasi A (1978) The toxicity of paraquat, diquat and morfamquat. Hans Huber, Bern

    Google Scholar 

  • Sherman TD, Becerril JM, Matsumoto H, Duke MV, Jacobs JM, Jacobs NJ, Duke SO (1991) Physiological basis for differential sensitivities of plant species to protoporphyrinogen oxidase-inhibiting herbicides. Plant Physiol 97:280–287

    Article  PubMed  CAS  Google Scholar 

  • The Angiosperm Phylogeny Group (2009) An update of the angiosperm phylogeny group classification for the orders and families of flowering plants: APG III. Bot J Linn Soc 161:105–121

    Article  Google Scholar 

  • Thompson JE, Legge RL, Barber RF (1987) The role of free radicals in senescence and wounding. New Phytol 105:317–344

    Article  CAS  Google Scholar 

  • Vanacker H, Carver TLW, Foyer CH (2000) Early H2O2 accumulation in mesophyll cells leads to induction of glutathione during the hyper-sensitive response in the barley-powdery mildew interaction. Plant Physiol 123:1289–1300

    Article  PubMed  CAS  Google Scholar 

  • Wise RR (1995) Chilling-enhanced photooxidation: the production, action and study of reactive oxygen species produced during chilling in the light. Photosynth Res 45:79–97

    Article  CAS  Google Scholar 

  • Yoon JY, Shin JS, Shin DY, Hyun KH, Burgos NR, Lee S, Kuk YI (2011) Tolerance to paraquat-mediated oxidative and environmental stresses in squash (Cucurbita spp.) leaves of various ages. Pestic Biochem Physiol 99:65–76

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by the Korea Research Foundation Grant funded by the Korean Government (331-2008-1-F00003).

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Correspondence to Yong In Kuk.

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Lee, S., Yoon, J.Y., Jung, H.I. et al. Ameliorative effects of squash (Cucurbita moschata Duchesne ex Poiret) leaf extracts on oxidative stress. Plant Growth Regul 67, 9–17 (2012). https://doi.org/10.1007/s10725-011-9655-1

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  • DOI: https://doi.org/10.1007/s10725-011-9655-1

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