Plant Growth Regulation

, Volume 65, Issue 2, pp 273–278 | Cite as

Arbuscular mycorrhizal fungi can alter some root characters and physiological status in trifoliate orange (Poncirus trifoliata L. Raf.) seedlings

  • Qiang-Sheng WuEmail author
  • Ying-Ning Zou
  • Xin-Hua He
  • Peng Luo
Original paper


Citrus plants strongly depend on mycorrhizal symbiosis because of less or no root hairs, but few reports have studied if their root traits and physiological status could be altered by different arbuscular mycorrhizal fungi (AMF). In a pot experiment we evaluated the effects of three AMF species, Glomus mosseae, G. versiforme and Paraglomus occultum on the root traits and physiological variables of the trifoliate orange (Poncirus trifoliata L. Raf.) seedlings. Root mycorrhizal colonization was 58–76% after 180 days of inoculation. AMF association significantly increased plant height, stem diameter, leaf number per plant, shoot and root biomass. Mycorrhizal seedlings also had higher total root length, total root projected area, total root surface area and total root volume but thinner root diameter. Among the three AMFs, greater positive effects on aboveground growth generally ranked as G. mosseae > P. occultum > G. versiforme, whilst on root traits as G. mosseae ≈ P. occultum > G. versiforme. Compared to the non-mycorrhizal seedlings, contents of chlorophyll, leaf glucose and sucrose, root soluble protein were significantly increased in the mycorrhizal seedlings. In contrast, root glucose and sucrose, leaf soluble protein, and activity of peroxidase (POD) in both leaves and roots were significantly decreased in the mycorrhizal seedlings. It suggested that the improvement of root traits could be dependent on AMF species and be related to the AMF-induced alteration of carbohydrates and POD.


Arbuscular mycorrhizal fungi Carbohydrate Peroxidase Root traits Soluble protein Trifoliate orange 



This work was supported by the National Natural Science Foundation of China (No.: 30800747), the Key Project of Chinese Ministry of Education (No.: 211107), and the Science-Technology Research Project for Excellent Middle-aged and Young Talents of Hubei Provincial Department of Education, China (No.: Q20111301).


  1. Amako K, Chen GX, Asade K (1994) Separate assays specific for ascorbate peroxidase and guaiacol peroxidase and for the chloroplastic and cytosolic isozymes of ascorbate peroxidase in plants. Plant Cell Physiol 35:497–504Google Scholar
  2. Araim G, Saleem A, Arnason JT, Charest C (2009) Root colonization by an arbuscular mycorrhizal (AM) fungus increases growth and secondary metabolism of purple coneflower, Echinacea purpurea (L.) Moench. J Agric Food Chem 57:2255–2258PubMedCrossRefGoogle Scholar
  3. Atkinson D, Black KE, Forbes PJ, Hooker JE, Baddeley JA, Watson CA (2003) The influence of arbuscular mycorrhizal colonization and environment on root development in soil. Eur J Soil Sci 54:751–757CrossRefGoogle Scholar
  4. Augín O, Mansilla JP, Vilariño A, Sainz M (2004) Effects of mycorrhizal inoculation on root morphology and nursery production of three grapevine rootstocks. Am J Enol Vitic 55:108–111Google Scholar
  5. Berta G, Trotta A, Fusconi A, Hooker JE, Munro M, Atkinson D, Giovannetti M, Morini S, Fortuna P, Tisserant B (1995) Arbuscular mycorrhizal induced changes to plant growth and root system morphology in Prunus cerasifera. Tree Physiol 15:281–293PubMedGoogle Scholar
  6. Boucher A, Dalpé Y, Charest C (1999) Effect of arbuscular mycorrhizal colonization of four species of Glomus on physiological responses of maize. J Plant Nutri 22:783–797CrossRefGoogle Scholar
  7. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254PubMedCrossRefGoogle Scholar
  8. Chen LM, Cheng JT, Chen EL, Yiu TJ, Liu ZH (2002) Naphthaleneacetic acid suppresses peroxidase activity during the induction of adventitious roots in soybean hypocotyls. J Plant Physiol 159:1349–1354CrossRefGoogle Scholar
  9. Gavito ME, Curtis PS, Jakobsen I (2001) Neither mycorrhizal inoculation nor atmospheric CO2 concentration has strong effects on pea root production and root loss. New Phytol 149:283–290CrossRefGoogle Scholar
  10. Ghorbanli M, Ebrahimzadeh H, Sharifi M (2004) Effects of NaCl and mycorrhizal fungi on antioxidative enzymes in soybean. Biol Plant 48:575–581CrossRefGoogle Scholar
  11. Gutjahr C, Casieri L, Paszkowski U (2009) Glomus intraradices induces changes in root system architecture of rice independently of common symbiosis signaling. New Phytol 182:829–837PubMedCrossRefGoogle Scholar
  12. Hodge A, Berta G, Doussan C, Merchan F, Crespi M (2009) Plant root growth, architecture and function. Plant Soil 321:153–187CrossRefGoogle Scholar
  13. Hooker JE, Munro M, Atkinson D (1992) Vesicular-arbuscular mycorrhizal fungi induced alteration in poplar root system morphology. Plant Soil 145:207–214CrossRefGoogle Scholar
  14. Ingram PA, Malamy JE (2010) Root system architecture. In: Kader JC, Delseny M (eds) Advances in botanical research, vol 55. Elsevier, New York, pp 75–117Google Scholar
  15. Jebara S, Jebara M, Limam F, Aouani ME (2005) Changes in ascorbate peroxidase, catalase, guaiacol peroxidase and superoxide dismutase activities in common bean (Phaseolus vulgaris) nodules under salt stress. J Plant Physiol 162:929–936PubMedCrossRefGoogle Scholar
  16. Lepeduš H, Cesar V, Krsnik-Rasol M (2004) Guaiacol peroxidases in carrot (Daucus carota L.) root. Food Technol Biotechnol 42:33–36Google Scholar
  17. Lichtenthaler K (1987) Chlorophyll and carotinoids: pigments of photosynthetic brommembranes. Method Enzymol 148:351–382Google Scholar
  18. Manoharan PT, Pandi M, Shanmugaiah V, Gomathinayagam S, Balasubramanian N (2008) Effect of vesicular arbuscular mycorrhizal fungus on the physiological and biochemical changes of five different tree seedlings grown under nursery conditions. Afr J Biotechnol 7:3431–3436Google Scholar
  19. Metaxas D, Syros T, Yupsanis T, Economous AS (2004) Peroxidases during adventitious rooting in cuttings of Arbutus unedo and Taxus baccata as affected by plant genotype and growth regulator treatment. Plant Growth Regul 44:257–266CrossRefGoogle Scholar
  20. Miransari M (2010) Contribution of arbuscular mycorrhizal symbiosis to plant growth under different types of soil stress. Plant Biol 12:563–569PubMedGoogle Scholar
  21. Murkute AA, Sharma S, Singh SK (2006) Studies on salt stress tolerance of citrus rootstock genotypes with arbuscular mycorrhizal fungi. Hort Sci 33:70–76Google Scholar
  22. Norman JR, Atkinson D, Hooker JE (1996) Arbuscular mycorrhizal fungal-induced alteration to root architecture in strawberry and induced resistance to the root pathogen Phytophthora fragariae. Plant Soil 185:191–198CrossRefGoogle Scholar
  23. Orfanoudakis M, Wheeler CT, Hooker JE (2010) Both the arbuscular mycorrhizal fungus Gigaspora rosea and Frankia increase root system branching and reduce root hair frequency in Alnus glutinosa. Mycorrhiza 20:117–126PubMedCrossRefGoogle Scholar
  24. Osmont KS, Sibout R, Hardtke CS (2007) Hidden branches: developments in root system architecture. Annu Rev Plant Biol 58:93–113PubMedCrossRefGoogle Scholar
  25. Padilla IMG, Encina CL (2005) Changes in root morphology accompanying mycorrhizal alleviation of phosphorus deficiency in micropropagated Annona cherimola Mill. Plant Sci Hortic 106:360–369CrossRefGoogle Scholar
  26. Phillips JM, Hayman DS (1970) Improved producers for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Trans Br Mycol Soc 55:158–161CrossRefGoogle Scholar
  27. Rolland F, Baena-Gonzalez E, Sheen J (2006) Sugar sensing and signaling in plants: conserved and novel mechanisms. Ann Rev Plant Physiol 57:675–709Google Scholar
  28. Schellenbaum L, Berta G, Ravolanirina F, Tisserant B, Gianinazzi S, Fitter AH (1991) Influence of endomycorrhizal infection on root morphology in a micropropagated woody plant species (Vitis vinifera L.). Ann Bot 68:135–141Google Scholar
  29. Smith SE, Read DJ (2008) Mycorrhizal symbiosis. 3rd. Academic Press, San DiegoGoogle Scholar
  30. Wu QS, Xia RX, Zou YN (2006) Reactive oxygen metabolism in mycorrhizal and non-mycorrhizal citrus (Poncirus trifoliata) seedlings subjected to water stress. J Plant Physiol 163:1101–1110PubMedCrossRefGoogle Scholar
  31. Wu QS, Xia RX, Zou YN (2008) Improved soil structure and citrus growth after inoculation with three arbuscular mycorrhizal fungi under drought stress. Eur J Soil Biol 44:122–128CrossRefGoogle Scholar
  32. Wu QS, Levy Y, Zou YN (2009) Arbuscular mycorrhizae and water relations in citrus. In: Tennant P, Benkeblia N (eds) Citrus II. Tree and forestry science and biotechnology 3:105–112Google Scholar
  33. Wu QS, Zou YN, He XH (2010) Contributions of arbuscular mycorrhizal fungi to growth, photosynthesis, root morphology and ionic balance of citrus seedlings under salt stress. Acta Physiol Plant 32:297–304CrossRefGoogle Scholar
  34. Yao Q, Wang LR, Zhu HH, Chen JZ (2009) Effect of arbusuclar mycorrhizal fungal inoculation on root system architecture of trifoliate orange (Poncirus trifoliata L. Raf.) seedlings. Sci Hortic 121:458–461CrossRefGoogle Scholar
  35. Zai XM, Qin P, Wan SW, Zhao FG, Wang G, Yan DL, Zhou J (2007) Effects of arbuscular mycorrhizal fungi on the rooting and growth of beach plum (Prunus maritima) cuttings. J Hortic Sci Biotech 82:863–866Google Scholar
  36. Zangaro W, Nishidate FR, Vandresen J, Andrade G, Nogueira MA (2007) Root mycorrhizal colonization and plant responsiveness are related to root plasticity, soil fertility and successional status of native woody species in southern Brazil. J Trop Ecol 23:53–62CrossRefGoogle Scholar
  37. Zhang ZL, Zai LJ (2004) Expermental instructment of plant physiology (in Chinese), 3rd edn. Higher Education Press, BeijingGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Qiang-Sheng Wu
    • 1
    Email author
  • Ying-Ning Zou
    • 1
  • Xin-Hua He
    • 2
    • 3
  • Peng Luo
    • 1
  1. 1.College of Horticulture and GardeningYangtze UniversityJingzhouPeople’s Republic China
  2. 2.Centre for Ecosystem Management/School of Natural SciencesEdith Cowan UniversityJoondalupAustralia
  3. 3.State Centre of Excellence for EcohydrologyUniversity of Western AustraliaCrawleyAustralia

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