Abstract
To understand details about the molecular mechanisms of abscisic acid (ABA) action in fruit maturation, one partial cDNA (PaCHLH1) encoding magnesium chelatase H subunit (CHLH) which was proven to be an ABA receptor in Arabidopsis was cloned from sweet cherry fruit. The sequence of PacCHLH1 was 442 bp. The amino acid sequence of PacCHLH1 shows high homology to other CHLHs. PacCHLH1 expression dramatically declined after 25 days after full bloom (DAFB) which is the beginning of the ripening process, in contrast to the changes in ABA levels during this period. The application of ABA promoted endogenous ABA biosynthesis and fruit ripening but significantly decreased the expression of PacCHLH1 in fruits. No significant correlation was observed between the PacCHLH1 transcript and ABA accumulation in dehydrated fruits. In dehydrated leaves, the ABA and PacCHLH1 transcript levels were both up-regulated.
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References
Coombe BG (1976) The development of flesh fruits. Annu Rev Plant Physiol 27:507–518. doi:10.1146/annurev.pp.27.060176.001231
Coombe BG, Hale CR (1973) The hormone content of ripening grape berries and the effects of growth substance treatments. Plant Physiol 51:629–634
Davies WJ, Zhang J (1991) Root signals and the regulation of growth and development of plants in drying soil. Annu Rev Plant Physiol Plant Mol Biol 42:55–76. doi:10.1146/annurev.pp.42.060191.000415
Finkelstein RR, Gampala SS, Rock CD (2002) Abscisic acid signaling in seeds and seedlings. Plant Cell 14:S15–S45. doi:10.1105/tpc.010441
Giraudat J, Parcy F, Bertauche N, Gosti F, Leung J (1994) Current advances in abscisic acid action and signaling. Plant Mol Biol 26:1557–1577
Karger GA, Reid JD, Hunter CN (2001) Characterization of the binding of deuteroporphyrin IX to the magnesium chelatase H subunit and spectroscopic properties of the complex. Biochemistry 40:9291–9299. doi:10.1021/bi010562a
Kondo S, Gemma H (1993) Relationship between abscisic acid (ABA) content and maturation of the sweet cherry. J Jpn Soc Hortic Sci 62:63–68
Kondo S, Inoue K (1997) Abscisic acid and 1-aminocyclopropane-1-carboxylic acid (ACC) content during growth of ‘Satohnishiki’ cherry fruit, and the effect of ABA and ethephon application on fruit quality. J Hortic Sci 72:221–227
Leung J, Giraudat J (1998) Abscisic acid signal transduction. Ann Rev Plant Physiol Plant Mol Biol 49:199–222. doi:10.1146/annurev.arplant.49.1.199
Michael HB, Valeria MS (1993) Future directions in plant hormone research. J Plant Growth Regul 12:227–235
Müller AH, Hansson M (2009) The barley magnesium chelatase 150-kD subunit is not an abscisic acid receptor. Plant Physiol 150:157–166. doi:10.1104/pp.109.135277
Rabino I, Mancinelli AL (1986) Light, temperature and anthocyanin production. Plant Physiol 81:922–924
Shen YY, Wang XF, Wu FQ, Du SY, Cau Z, Shang Y, Wang XL, Peng CC, Yu XC, Zhu SY, Fan RC, Xu YH, Zhang DP (2006) The Mg-chelatase H subunit is an abscisic acid receptor. Nature 443:823–826. doi:10.1038/nature05176
Walker CJ, Willows RD (1997) Mechanism and regulation of Mg-chelatase. Biochem J 327:321–333
Wan CY, Wilkins TA (1994) A modified hot borate method significantly enhances the yield of high-quality RNA from cotton (Gossypium hirsutum L.). Anal Biochem 223:7–12. doi:10.1006/abio.1994.1538
Wang XF, Zhang DP (2007) Abscisic acid receptors: multiple signal perception sites. Ann Bot 101:311–317. doi:10.1093/aob/mcm284
Wu FQ, Xin Q, Cao Z, Liu ZQ, Du SY, Mei C, Zhao CX, Wang XF, Shang Y, Jiang T, Zhang XF, Yan L, Zhao R, Cui ZN, Liu R, Sun HL, Yang XL, Su Z, Zhang DP (2009) The magnesium-chelatase H subunit binds abscisic acid and functions in abscisic acid signaling: new evidence in Arabidopsis. Plant Physiol 150:1940–1954. doi:10.1104/pp.109.140731
Zhang DP, Zhang Z, Chen J, Jia WS (1999) Specific abscisic acid-binding sites in mesocarp of grape berry: properties and sub-cellular localization. J Plant Physiol 155:324–331
Zhang DP, Chen SW, Peng YB, Shen YY (2001) Abscisic acid-specific binding sites in the flesh of developing apple fruit. J Exp Bot 52:2097–2103
Zhang M, Yuan B, Leng P (2009a) The role of ABA in triggering ethylene biosynthesis and ripening of tomato fruit. J Exp Bot 60:1579–1588. doi:10.1093/jxb/erp026
Zhang M, Leng P, Zhang GL, Li XX (2009b) Cloning and functional analysis of 9-cis-epoxycarotenoid dioxygenase (NCED) genes encoding a key enzyme during abscisic acid biosynthesis from peach and grape fruits. J Plant Physiol 166:1241–1252. doi:10.1016/j.jplph.2009.01.013
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Ren, J., Sun, L., Wang, C. et al. Expression analysis of the cDNA for magnesium chelatase H subunit (CHLH) during sweet cherry fruit ripening and under stress conditions. Plant Growth Regul 63, 301–307 (2011). https://doi.org/10.1007/s10725-010-9530-5
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DOI: https://doi.org/10.1007/s10725-010-9530-5