Skip to main content

Advertisement

SpringerLink
Log in

Stress-associated factors increase after desiccation of germinated seeds of Tabebuia impetiginosa Mart.

  • SI Plant Desiccation Stress
  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

Improved re-establishment of desiccation tolerance was studied in germinated seeds of Tabebuia impetiginosa Mart. by exposing to a polyethylene glycol solution prior to desiccation. The effects of different osmotic potentials and drying rates were studied. In addition, temporary temperature stress and exogenous abscisic acid were applied to evaluate their effect on desiccation tolerance of the protruded radicle. An osmotic potential of −1.7 MPa at 5°C followed by slow drying was most effective in the re-establishment of desiccation tolerance in protruded radicles with a length up to 3 mm. An osmotic potential of −1.4 or −2.0 MPa was less effective. Fast drying completely prevented the re-induction of desiccation tolerance. Cold shock or heat shock prior to osmotic treatment as well as abscisic acid added to the osmotic solution improved desiccation tolerance of protruded radicles. Surprisingly, survival of the germinated seed did not depend on re-establishment of desiccation tolerance in the protruded radicle. Even after the protruded radicle became necrotic and died, the production of adventitious roots from the hypocotyls allowed for survival and the development of high quality seedlings. Thus, T. impetiginosa appeared to be well adapted to the seasonally dry biome in which the species thrives via mechanisms that offer protection against desiccation in the young seedling stage.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Berjak P (2006) Unifying perspectives of some mechanisms basic to desiccation tolerance across life forms. Seed Sci Res 16:1–15

    Article  CAS  Google Scholar 

  • Boudet J, Buitink J, Hoekstra FA, Rogniaux H, Larre C, Satour P, Leprince O (2006) Comparative analysis of the heat stable proteome of radicles of Medicago truncatula seeds during germination identifies late embryogenesis abundant proteins associated with desiccation tolerance. Plant Physiol 140:1418–1436

    Article  CAS  PubMed  Google Scholar 

  • Bruggink T, van der Toorn P (1995) Induction of desiccation tolerance in germinated seeds. Seed Sci Res 5:1–4

    Article  Google Scholar 

  • Buitink J, Vu BL, Satour P, Leprince O (2003) The re-establishment of desiccation tolerance in germinated radicles of Medicago truncatula Gaertn. seeds. Seed Sci Res 13:273–286

    Article  CAS  Google Scholar 

  • Buitink J, Leger JJ, Guisle I, Vu BL, Wuilleme S, Lamirault G, Le Bars A, Le Meur N, Becker A, Kuester H, Leprince O (2006) Transcriptome profiling uncovers metabolic and regulatory processes occurring during the transition from desiccation-sensitive to desiccation-tolerant stages in Medicago truncatula seeds. Plant J 47:735–750

    Article  CAS  PubMed  Google Scholar 

  • de Carvalho LR, da Silva EAA, Davide AC (2006) Classificação de sementes florestais quanto ao comportamento no armazenamento. Revista Brasileira de Sementes 28:15–25

    Article  Google Scholar 

  • Faria JMR, Buitink J, van Lammeren AAM, Hilhorst HWM (2005) Changes in DNA and microtubules during loss and re-establishment of desiccation tolerance in germinating Medicago truncatula seeds. J Exp Bot 56:2119–2130

    Article  CAS  PubMed  Google Scholar 

  • Farrant JM, Berjak P, Pammenter NW (1987) Ecological significance of three-phase production of roots during germination and establishment of the recalcitrant propagules of Avicennia marina. S Afr J Sci 83:236–237

    Google Scholar 

  • Górecki RJ, Piotrowicz AI, Lahuta LB, Obendorf RL (1997) Soluble carbohydrates in desiccation tolerance of yellow lupin seeds during maturation and germination. Seed Sci Res 7:107–115

    Google Scholar 

  • Hoekstra FA, Golovina EA, Buitink J (2001) Mechanisms of plant desiccation tolerance. Trends Plant Sci 6:431–438

    Article  CAS  PubMed  Google Scholar 

  • ISTA (1996) International rules for seed testing. Seed Sci Technol 24(Suppl):48–52

    Google Scholar 

  • Kermode AR, Finch-Savage BE (2002) Desiccation sensitivity in orthodox and recalcitrant seeds in relation to development. In: Black M, Pritchard HW (eds) Desiccation sensitivity in orthodox and recalcitrant seeds in relation to development, 1st edn. CABI Publishing, Wallingford, pp 149–184

    Google Scholar 

  • Köppen W (1936) Das Geographische System der Klimatologie. Bornträger Verl, Berlin

    Google Scholar 

  • Koster KL, Leopold AC (1988) Sugars and desiccation tolerance in seeds. Plant Physiol 88:829–832

    Article  CAS  PubMed  Google Scholar 

  • Leprince O, Harren FJM, Buitink J, Alberda M, Hoekstra FA (2000) Metabolic dysfunction and unabated respiration precede the loss of membrane integrity during dehydration of germinating radicles. Plant Physiol 122:597–608

    Article  CAS  PubMed  Google Scholar 

  • Lin T-P, Yen W-L, Chien C-T (1998) Disappearance of desiccation tolerance of imbibed crop seeds is not associated with the decline of oligosaccharides. J Exp Bot 49:1203–1212

    Article  CAS  Google Scholar 

  • Mckee JMT, Finch-Savage WE (1989) The effect of abscisic acid on the growth and storage of germinating rape (Brassica napus L.) seed dried following selection on the basis of a newly-emerged radicle. Plant Growth Regul 8:77–83

    CAS  Google Scholar 

  • Michel BE (1983) Evaluation of the water potentials of solutions of polyethylene glycol 8000 both in the absence and presence of other solutes. Plant Physiol 72:66–70

    Article  CAS  PubMed  Google Scholar 

  • Oliver MJ, Wood AJ, O’Mahony P (1998) “To dryness and beyond”—preparation for the dried state and rehydration in vegetative desiccation-tolerant plants. Plant Growth Regul 24:193–201

    Article  CAS  Google Scholar 

  • Roberts EH (1973) Predicting the storage of life seeds. Seed Sci Technol 1:499–514

    Google Scholar 

  • Sabehat A, Weiss D, Lurie S (1998) Heat-shock proteins and cross-tolerance in plants. Physiol Plant 103:437–441

    Article  CAS  Google Scholar 

  • Sampaio SC, Corrêa MM, Vilas Bôas MA, Coutinho de Oliveira LF (2000) Estudo da precipitação efetiva para o município de Lavras, MG. Revista Brasileira de Engenharia Agrícola e Ambiental 4:210–213

    Google Scholar 

  • Senaratna T, McKersie BD (1983) Dehydration injury in germinating soybean (Glycine max L. Merr.) seeds. Plant Physiol 72:620–624

    Article  CAS  PubMed  Google Scholar 

  • Senaratna T, McKersie BD, Bowley SR (1989) tolerance of alfalfa (Medicago sativa L.) somatic embryos. Influence of abscisic acid, stress pretreatments and drying rates. Plant Sci 65:253–259

    Article  CAS  Google Scholar 

  • Sun WQ (1999) Desiccation sensitivity of recalcitrant seeds and germinated orthodox seeds: can germinated orthodox seeds serve as a model system for studies of recalcitrance? In: Marzalina M, Khoo KC, Jayanthi N, Tsan FY, Krishnapillay B (eds) Recalcitrant seeds, Proceedings of IUFRO seed symposium 1998. Forest Research Institute, Kuala Lumpur, pp 29–42

    Google Scholar 

  • Vierling E (1991) The roles of heat shock proteins in plants. Annu Rev Plant Physiol Plant Mol Biol 42:579–620

    Article  CAS  Google Scholar 

  • Walters C, Ried JL, Walker-Simmons MK (1997) Heat-soluble proteins extracted from wheat embryos have tightly bound sugars and unusual hydration properties. Seed Sci Res 7:125–134

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank the Kew Latin America Research programme for providing a fellowship to Carlos Vieira. We also thank John Adams and other staff at the Millennium Seed Bank for assistance.

Author information

Author notes

  1. Carlos Vinicio Vieira

    Present address: Departamento de Agronomia, Universidade Federal de Matto Grosso, Matto Grosso, Brazil

Authors and Affiliations

  1. Setor de Fisiologia Vegetal, Departamento de Biologia, Universidade Federal de Lavras, Minas Gerais, Brazil

    Carlos Vinicio Vieira, Amauri Alves de Alvarenga & Evaristo Mauro de Castro

  2. Laboratório de Sementes Florestais, Departamento de Ciências Florestais, Universidade Federal de Lavras, Minas Gerais, Brazil

    Edvaldo A. Amaral da Silva

  3. Seed Conservation Department, Royal Botanic Gardens, Kew, Wakehurst Place, Ardingly, West Sussex, RH17 6TN, UK

    Peter E. Toorop

Authors
  1. Carlos Vinicio Vieira
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Edvaldo A. Amaral da Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Amauri Alves de Alvarenga
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Evaristo Mauro de Castro
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Peter E. Toorop
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Peter E. Toorop.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vieira, C.V., Amaral da Silva, E.A., de Alvarenga, A.A. et al. Stress-associated factors increase after desiccation of germinated seeds of Tabebuia impetiginosa Mart.. Plant Growth Regul 62, 257–263 (2010). https://doi.org/10.1007/s10725-010-9496-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10725-010-9496-3

Keywords

Search

Navigation