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Abscisic acid delays chickpea germination by inhibiting water uptake and down-regulating genes encoding cell wall remodelling proteins

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Abstract

Here, we compare the germination of chickpea seeds when they were imbibed in mannitol and in ABA. Radicle protusion was delayed by both treatments, however, while the negative effect of mannitol, which severely reduced earlier water uptake, was reversed after 24 h of treatment, this was not the case of ABA, indicating a specific effect of the hormone. Since germination depends not only on the water content but also on cell wall extensibility, we analyzed the rate of transcription of some genes encoding cell wall remodelling proteins, known to be involved in chickpea germination, in embryonic axes excised from seeds imbibed in mannitol or in ABA. Our results show that ABA and mannitol modify their transcription patterns. Among the α-expansins, the proteins encoded by CaEXPA-3 and CaEXPA-4 must be responsible for the germination observed in mannitol-treated seeds, because their transcripts appeared earlier on in mannitol- than in ABA-treated seeds. Moreover, it may further be concluded that CaXTH-1 is one of the genes able of transcription in very low hydrated seed.

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Abbreviations

CW:

Cell wall

DW:

Dry weight

FW:

Fresh weight

HAI:

Hours after imbibition

nIOD:

Normalized integrated optical density

RWC:

Relative water content

TW:

Turgid weight

WC:

Water content

References

  • Aldasoro JJ, Matilla A, Nicolás G (1981) Effect of ABA, FC and TU on germination and K+ and glucose uptake in chickpea seeds at different temperatures. Physiol Plant 53:139–145

    Article  CAS  Google Scholar 

  • Barrôco RM, Van Poucke K, Bergervoet JHW, De Veylder L, Groot SPC, Inzè D, Engler G (2005) The role of the cell cycle machinery in resumption of postembryonic development. Plant Physiol 137:127–140

    Article  PubMed  CAS  Google Scholar 

  • Bassel GW, Fung P, Freeman Chow T-f, Foong JA, Provart NJ, Cutler SR (2008) Elucidating the germination transcriptional program using small molecules. Plant Physiol 147:143–155

    Article  CAS  PubMed  Google Scholar 

  • Bewley JD (1997) Seed germination and dormancy. Plant Cell 9:1055–1066

    Article  CAS  PubMed  Google Scholar 

  • Bewley JD, Black M (1994) Seeds. Physiology of development and germination. Plenum Press, New York

    Google Scholar 

  • Bradford KJ (1995) Water relations in seed germination. In: Kigel J, Galili G (eds) Seed development and germination. Marcel Dekker Inc, New York, pp 351–396

    Google Scholar 

  • Chibani K, Li-Rachedi S, Job C, Job D, Jullien M, Grappin P (2006) Proteomic analysis of seed dormancy in Arabidopsis. Plant Physiol 142:1493–1510

    Article  CAS  PubMed  Google Scholar 

  • Comai L, Harada JJ (1990) Transcriptional activities in dry seed nuclei indicate the timing of the transition from embryogeny to germination. Proc Natl Acad Sci USA 87:2671–2674

    Article  CAS  PubMed  Google Scholar 

  • Cosgrove DJ (2000) Loosening of plant cell walls by expansins. Nature 407:321–326

    Article  CAS  PubMed  Google Scholar 

  • Esteban R, Labrador E, Dopico B (2005) A family of β-galactosidase cDNAs related to development of vegetative tissue in Cicer arietinum. Plant Sci 168:457–460

    Article  CAS  Google Scholar 

  • Gao Y, Zeng Q, Guo J, Cheng J, Ellis BE, Chen J-G (2007) Genetic characterization reveals no role for the reported ABA receptor, GCR2, in ABA control of seed germination and early seedling development in Arabidopsis. Plant J 52:1001–1013

    Article  CAS  PubMed  Google Scholar 

  • González L, González-Vilar M (2001) Determination of relative water content. In: Reigosa MJ (ed) Handbook of plant ecophysiology techniques. Kluwer Academic Publisher, Dordrecht, pp 207–211

    Google Scholar 

  • Hernández-Nistal J, Rodríguez D, Nicolás G, Aldasoro JJ (1989) Abscisic acid and temperature modify the levels of calmodulin in embryonic axes of Cicer arietinum. Physiol Plant 75:255–260

    Article  Google Scholar 

  • Hernández-Nistal J, Labrador E, Martín I, Jiménez T, Dopico B (2006) Transcriptional profiling of cell wall protein genes in chickpea embryonic axes during germination and growth. Plant Physiol Biochem 44:684–692

    Article  PubMed  CAS  Google Scholar 

  • Holdsworth MJ, Bentsink L, Soppe WJJ (2008) Molecular networks regulating Arabidopsis seed maturation, after ripening, dormancy and germination. New Phytol 179:33–54

    Article  CAS  PubMed  Google Scholar 

  • Jiménez T, Martín I, Labrador E, Dopico B (2006) The immunolocation of a xyloglucan endotransglucosylase/hydrolase specific to elongating tissues in Cicer arietinum suggest a role in the elongation of vascular cells. J Exp Bot 57:3979–3988

    Article  PubMed  CAS  Google Scholar 

  • Karssen CM (1995) Hormonal regulation of seed development, dormancy, and germination studied by genetic control. In: Kigel J, Galili G (eds) Seed development and germination. Marcel Dekker Inc, New York, pp 333–350

    Google Scholar 

  • Koornneef M, Bentsink L, Hilhorst H (2002) Seed dormancy and germination. Curr Opin Plant Biol 5:33–36

    Article  CAS  PubMed  Google Scholar 

  • Kucera B, Cohn MA, Leubner-Metzger G (2005) Plant hormone interactions during seed dormancy release and germination. Seed Sci Res 15:281–307

    Article  CAS  Google Scholar 

  • Labrador E, Rodríguez D, Nicolás G (1987) Changes in cell wall composition of embryonic axes of germinating Cicer arietinum L. seeds. Effects of abscisic acid and temperature. Plant Sci 48:23–30

    Article  CAS  Google Scholar 

  • Leubner-Metzger G (2005) β-1, 3-glucanase gene expression in low hydrated seeds as a mechanism for dormancy release during tobacco after-ripening. Plant J 41:133–145

    Article  CAS  PubMed  Google Scholar 

  • Leubner-Metzger G, Fründt C, Vögeli-Lange R, Meins F (1995) Class β-1, 3 glucanase in the endosperm of tobacco during germination. Plant Physiol 109:751–759

    CAS  PubMed  Google Scholar 

  • Müller K, Tintelnot S, Leubner-Metzger G (2006) Endosperm-limited Brassicaceae seed germination: abscisic acid inhibits embryo induced endosperm weakening of Lepidium sativum (cress) and endosperm rupture of cress and Arabidopsis thaliana. Plant Cell Physiol 47:864–877

    Article  PubMed  CAS  Google Scholar 

  • Muñoz FJ, Dopico B, Labrador E (1997) Two growth-related organ-specific cDNAs from Cicer arietinum epicotyls. Plant Mol Biol 35:433–442

    Article  PubMed  Google Scholar 

  • Nishitani K (2002) Genome-based approach to study the mechanism by which cell-wall type is defined and constructed by means of collaborative actions of wall-related enzymes. J Plant Res 115:303–307

    Article  CAS  PubMed  Google Scholar 

  • Rajjou L, Gallardo K, Debeaujon I, Vandekerckhove J, Job C, Job D (2004) The effect of α-amanitina on the Arabidopsis seed proteome highlights the distint roles of stored and neosynthesized mRNAs during germination. Plant Physiol 134:1598–1613

    Article  CAS  PubMed  Google Scholar 

  • Romo S, Dopico B, Labrador E (2002) The expression of a new Cicer arietinum cDNA, encoding a glutamic acid-rich protein, is related to development. J Plant Physiol 159:1357–1381

    Article  Google Scholar 

  • Romo S, Jiménez T, Labrador E, Dopico B (2005) The gene for a xyloglucan endotransglucosylase/hydrolase from Cicer arietinum is strongly expressed in elongating tissues. Plant Physiol Biochem 43:169–176

    Article  CAS  PubMed  Google Scholar 

  • Sánchez MA, Mateos I, Labrador E, Dopico B (2004) Brassinolides and IAA induce the transcription of four α-expansin genes related to development in Cicer arietinum. Plant Physiol Biochem 24:709–716

    Article  CAS  Google Scholar 

  • Schopfer P, Plachy C (1984) Control of seed germination by abscisic acid. II. Effect on embryo water uptake in Brassica napus L. Plant Physiol 76:155–160

    Article  CAS  PubMed  Google Scholar 

  • Slatyer RO (1967) Plant water relationships. Academic Press, New York

    Google Scholar 

  • Thompson JE, Fry SC (2001) Density-labelling of cell wall polysaccharides in cultured rose cells: comparison of incorporation of 3H and 13C from exogenous glucose. Carbohydr Res 332:175–182

    Article  CAS  PubMed  Google Scholar 

  • Urano K, Maruyama K, Ogata Y, Morishita Y, Takeda M, Sakurai N, Suzuki H, Saito K, Shibata D, Kobayashi M, Yamaguchi-Shinozaki K, Shinozaki K (2009) Characterization of the ABA-regulated global responses to dehydration in Arabidopsis by metabolomics. Plant J 57:1065–1078

    Article  CAS  PubMed  Google Scholar 

  • Welbaum GE, Bradford KJ, Kyu-Ock Y, Booth DT, Oluoch MO (1998) Biophysical, physiological and biochemical processes regulating seed germination. Seed Sci Res 8:161–172

    Article  CAS  Google Scholar 

  • Wu Y, Thorne ET, Sharp E, Cosgrove DJ (2001) Modification of expansin transcript level in the maize primary root at low water potentials. Plant Physiol 126:1471–1479

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This work was supported by grants from the Ministerio de Educación y Ciencia, Spain (BFU2006-09786) and from the Junta de Castilla y León (SA103A07).

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Authors and Affiliations

  1. Departamento de Fisiología Vegetal, E.P.S, Universidad de Santiago de Compostela, Campus de Lugo, 27002, Lugo, Spain

    Josefina Hernández-Nistal

  2. Departamento de Fisiología Vegetal, Centro Hispano Luso de Investigaciones Agrarias, Universidad de Salamanca, Plaza de los Doctores de la Reina s/n, Campus Miguel de Unamuno, 37008, Salamanca, Spain

    Ignacio Martín, Berta Dopico & Emilia Labrador

  3. Departamento de Biología Molecular y Celular, Facultad de Ciencias Experimentales, IE Universidad, 40003, Segovia, Spain

    Rocío Esteban

Authors
  1. Josefina Hernández-Nistal
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  2. Ignacio Martín
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  3. Rocío Esteban
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  4. Berta Dopico
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  5. Emilia Labrador
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Corresponding author

Correspondence to Emilia Labrador.

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Hernández-Nistal, J., Martín, I., Esteban, R. et al. Abscisic acid delays chickpea germination by inhibiting water uptake and down-regulating genes encoding cell wall remodelling proteins. Plant Growth Regul 61, 175–183 (2010). https://doi.org/10.1007/s10725-010-9463-z

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