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The effect of thidiazuron level on in vitro regeneration type and peroxidase profile in Eucalyptus microtheca F. Muell

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Abstract

The green twigs of 1-year-old Eucalyptus microtheca F. Muell seedlings were cultured on modified MS medium, supplemented with α-naphthalene acetic acid (NAA) and kinetin (Kin) hormones at 12 different concentrations. After 4 weeks, the combination of 1 mg l−1 NAA + 1 mg l−1 Kin induced the highest number of axillary shoots. Meanwhile, embryogenic calli were observed in media containing 4 mg l−1 NAA + 0.5 mg l−1 Kin, without any regeneration. The hormone treatments were followed by subculturing the twigs in different levels of thidiazuron (TDZ). The combination of 1 mg l−1 NAA + 1 mg l−1 Kin together with 0.01 mg l−1 TDZ resulted in an increase of direct shoot, while higher amounts of TDZ led to adventitious shoot induction. Somatic embryogenesis was observed in the treatment containing 0.01 mg l−1 TDZ + 4 mg l−1 NAA + 0.5 mg l−1Kin. The peroxidase (POD) band patterns in regenerated plantlets were investigated in order to determine the effect of different levels of TDZ on loci synthesis. A dimer locus, a tetramer locus and two epigenetic bands (a new band for NAA + Kin and the other for TDZ) were observed in the POD profiles. In case of low (0.01 mg l−1 and 0.1 mg l−1) levels of TDZ, one heterozygote allele was disappeared from dimer locus, while at higher TDZ levels, the dimer locus lost its stability and tetramer locus showed a high activity. Thus, POD allele patterns seems to be a feasible marker for different types of regeneration.

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Abbreviations

Kin:

Kinetin

NAA:

α-Naphthalene acetic acid

POD:

Peroxidase

TDZ:

Thidiazuron

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Acknowledgments

This research was supported by funds from the Research Institute of Forests and Rangelands of Iran.

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Authors and Affiliations

  1. Department of Plant Breeding, Faculty of Agriculture and Natural Resources, Islamic Azad University, Karaj Branch, P.O. Box 31485–313, Karaj, Tehran, Iran

    Morteza Shabannejad Mamaghani

  2. Department of Biotechnology, Research Institute of Forests and Rangelands, P.O. Box 13185–116, Tehran, Iran

    Mohammad Hassan Assareh, Abbas Ghamari-Zare, Shokofeh Shahrzad & Massih Forootan

  3. Department of Plant Breeding, Faculty of Agriculture, University of Tehran, P.O. Box 31587–11167, Karaj, Tehran, Iran

    Mansoor Omidi

  4. Forest Research Division, Research Institute of Forests and Rangelands, P.O. Box 13185–116, Tehran, Iran

    Mohammad Matinizadeh

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  1. Morteza Shabannejad Mamaghani
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  2. Mohammad Hassan Assareh
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Correspondence to Morteza Shabannejad Mamaghani.

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Shabannejad Mamaghani, M., Assareh, M.H., Omidi, M. et al. The effect of thidiazuron level on in vitro regeneration type and peroxidase profile in Eucalyptus microtheca F. Muell. Plant Growth Regul 59, 199–205 (2009). https://doi.org/10.1007/s10725-009-9404-x

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  • DOI: https://doi.org/10.1007/s10725-009-9404-x

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