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WGA reduces the level of oxidative stress in wheat seedlings under salinity

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Abstract

Protective effect of exogenous wheat germ agglutinin (WGA) on wheat seedling (Triticum aestivum L.) during salinity stress was studied. In particular, we examined the state of pro- and antioxidant systems as well as the level of peroxide oxidation of lipids and electrolyte leakage under control conditions and when stressed with NaCl. Generation of superoxide anions and activity of both superoxide dismutase (SOD) and peroxidase increased during saline stress. Accumulation of O2 ·− resulted in peroxide oxidation of lipids and electrolyte leakage in response to stress. The injurious effect of salinity on root growth of seedlings was manifested by a decreased mitotic index (MI) in apical root meristem. This study show that WGA pretreatment decreased salt-induced superoxide anion generation, SOD and peroxidase activities, levels of lipid peroxidation and electrolytes leakage as well as correlating with a reduction in the inhibition of root apical meristem mitotic activity in salt-treated plants. This suggests that exogenous WGA reduced the detrimental effects of salinity-induced oxidative stress in wheat seedlings. Thus WGA effects on a balance of reactive oxygen species (ROS) and activities of antioxidant enzymes may provide an important contribution to a range of the defense reactions induced by this lectin in wheat plants.

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Acknowledgements

This research is supported by grants no. NSh-3692.2006.4 and no. MK-5476.2006.4.

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Authors and Affiliations

  1. Institute of Biochemistry and Genetics, Ufa Scientific Centre, Russian Academy of Sciences, pr. Octyabrya 71, 450054, Ufa, Russia

    Marina Bezrukova, Alsu Kildibekova & Farida Shakirova

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  1. Marina Bezrukova
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  2. Alsu Kildibekova
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  3. Farida Shakirova
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Correspondence to Farida Shakirova.

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Bezrukova, M., Kildibekova, A. & Shakirova, F. WGA reduces the level of oxidative stress in wheat seedlings under salinity. Plant Growth Regul 54, 195–201 (2008). https://doi.org/10.1007/s10725-007-9248-1

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  • DOI: https://doi.org/10.1007/s10725-007-9248-1

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