Abstract
Pyrethrins and flower yield of pyrethrum (Chrysanthemum cinerariaefolium Viz.) plants were determined after application of ethrel, chlormequat chloride and paclobutrazol. Ethrel at 50, 100, 250 and 500 mg l−l produced a significant positive effect on pyrethrins level, decreased plant height, while 50 and 100 mg l−l significantly increased fresh and dry flower yield. Chlormequat chloride at 1000 and 2000 mg l−l and paclobutrazol (80 and 160 mg l−l) increased pyrethrins level, single flower weight and decreased plant height and flower yield. 14C-acetate incorporation studies further substantiated positive effect of growth retardants on pyrethrins biosynthesis. The effect of growth retardants on pyrethrins seems to be mediated through its effect on biosynthesis.
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References
Berova M, Zlatev Z (2000) Physiological response and yield of paclobutrazol treated tomato plants (Lycopersicon esculentum Mill.). Plant Growth Regul 30:117–123
Bhat BK, Menary RC (1986) Scanning electron microscopic study of oil glands in pyrethrum flowers. Pyrethrum Post 15:11–15
Botha ML,Whitehead CS, Halevy AH (1998) Effect of octanoic acid on ethylene-mediated flower induction in Dutch iris. Plant Growth Regul 25:47–51
El-Keltawi NE, Croteau R (1986a) Influence of phosphon D and cycocel on growth and essential oil content of sage and peppermint. Phytochemistry 25:1603–1606
El-Keltawi NE, Croteau R (1986b) Influence of ethephon and daminozide on growth and essential oil content of peppermint and sage. Phytochemistry 25:1285–1288
El-Sayed M, Verpoorte R (2004) Growth, metabolic profiling and enzymes activities of Catharanthus roseus seedlings treated with plant growth regulators. Plant Growth Regul 44:53–58
Farooqi AHA, Shukla A, Sharma S, Khan A (1996) Effect of plant age and GA3 on artemisinin and essential oil yield in Artemisia annua L. J Herbs Spices Med Plants 4:73–80
Farooqi AHA, Kumar R, Khan A, Sharma S, Kumar S (1999) Effect of planting date on growth and development of pyrethrum (Chrysanthemum cinerariaefolium) under subtropical Indian climate. Pyrethrum Post 20:119–124
Farooqi AHA, Khan A, Sharma S (2003) Selection of suitable lines of Tanacetum cinerariaefolium (pyrethrum) for north Indian plains. Indian J Plant Physiol (special issue):737–742
Farooqi AHA, Fatima S, Khan A, Sharma S (2005) Ameliorative effect of chloromequat chloride and IAA on drought stressed cultivars of Cymbopogon martini and C. winterianus. Plant Growth Regul 46:277–284
Gomez P, Ortuno A, Del Rio JA (2004) Ultrastructural changes and diosgenin content in cell suspensions of Trigonella foenum-graecum by ethylene treatment. Plant Growth Regul 44:93–99
Hedden P (1990) The action of plant growth retardants at the biochemical level. In: Pharis RP, Rood SB (eds) Plant growth substances 1988, Springer-Verlag, Berlin, Heidelberg, New York, pp 322–332
Hitmi A, Sallanon H, Barthomeuf C (2003) Effects of plant growth regulators on the growth and pyrethrin production by cell cultures of Chrysanthemum cineraraiefolium. Aust J Bot 49:81–88
Hoagland DR, Arnon DI (1938) The water culture method for growing plants without soil. Circ.347, California Agric Exp St
Matsuda K, Kikuta Y, Haba A, Nakayama K, Katsuda Y, Hatanaka A, Komai K (2005) Biosynthesis of pyrethrin I in seedlings of Chrysanthemum cinerariaefolium. Phytochemistry 66:1529–1535
Mirjalili N, Linden JC (1996) Methyl jasmonate induced production of taxol in suspension cultures of Taxus cuspidata: ethylene interaction and induction models. Biotechnol Prog 12:108–110
Muturi SN, Parlevliet JE, Brewer JG (1969) Ecological requirements of Pyrethrum: A general review. Pyrethrum Post 10:24–28
Nickell LG (1982).Plant growth regulators—agricultural uses. Springer-Verlag, Berlin, Heidelberg, New York, pp 32–44
Odinga WA, Angedu A (2003) The relationship between pyrethrins and the yellow pigmentation in pyrethrum flowers. African J Sci Technol 4:116–123
Pandita PN (1984) Pyrethrum the insecticidal plant. Indian Hort 42:29–30
Panse VG, Sukhatme PV (1967) Statistical methods for agricultural workers. ICAR Publication, New Delhi
Parlevliet JE (1970) The effect of rainfall and altitude on the yield of pyrethrins from pyrethrum flowers in Kenya. Pyrethrum Post 10:20–25
Rajala A, Peltonen-Sainiio P, Onnela M, Jackson M (2002) Effects of applying stem-shortening plant growth regulators to leaves on root elongation by seedlings of wheat, oat and barley: mediation by ethylene. Plant Growth Regul 38:51–59
Rao BRR, Singh SP, Rao EVSP (1985) N, P, K fertilizer studies in pyrethrum (Chrysanthemum cinerariaefolium). J Agric Sci Cambridge 100:509–511
Salardini AA, Chapman KSR, Holloway RJ (1994) Effect of potassium fertilization of pyrethrum (Tanacetum cinerariaefolium) on yields, pyrethrins concentration in dry achenes and potassium concentration in soil and plant tissues. Aust J Agric Res 45:647–656
Sangwan NS, Farooqi AHA, Fatima S, Sangwan RS (2001) Regulation of essential oil production in plants. Plant Growth Regul 34:3–21
Sharma S, Farooqi AHA (1990) Effect of 2-chloro-ethylphosphonic acid on economic yield of damask rose (Rosa damascena). Indian J Agric Sci 60:691–692
Shukla A, Farooqi AHA, Shukla YN, Sharma S (1992) Effect of triacontanol and chlormequat on growth, plant hormones and artemisinin yield in Artemisia annua L. Plant Growth Regul 1:165–171
Singh N, Luthra L, Sangwan RS (1991) Mobilization of starch and essential oil biogenesis during leaf ontogeny of Lemongrass (Cymbopogon flexuosus Stapf.). Plant Cell Physiol 32:803–811
Singh P, Srivastava NK, Misra A, Sharma S (1999) Influence of ethrel and gibberellic acid on carbon metabolism, growth and essential oil accumulation in spearmint (Mentha spicata). Photosynthetica 36:509–517
Sponsel VM (2002) The deoxyxylulose phosphate pathway for the biosynthesis of plastidic isoprenoids: early days in our understanding of the early stages of gibberellin biosynthesis. J Plant Growth Regul 20:332–345
Wang IH, SubramanianV, Moorman R, Burlson J, Ko J (1997). Direct etermination of pyrethrins in pyrethrum extracts by reversed-phase high-performance liquid chromatography with diode-array detection. J Chrom A 766:277–281
Acknowledgements
The authors are thankful to the Director, Central Institute of Medicinal and Aromatic Plants (CIMAP) Lucknow, for providing the necessary facilities and Junior Research Fellowship from CSIR, New Delhi, to one of us (SH).
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Haque, S., Farooqi, A.H.A., Gupta, M.M. et al. Effect of ethrel, chlormequat chloride and paclobutrazol on growth and pyrethrins accumulation in Chrysanthemum cinerariaefolium Vis.. Plant Growth Regul 51, 263–269 (2007). https://doi.org/10.1007/s10725-007-9170-6
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DOI: https://doi.org/10.1007/s10725-007-9170-6