Abstract
The effect of heat stress, drought and abscisic acid (ABA) on the induction of boiling-stable proteins were studied in 12 h germinated sorghum embryos and endosperm. SDS-PAGE revealed the heat shock induction of one major 97 kDa boiling-stable protein both in embryos and endosperm. Western blotting analysis, using polyclonal antibodies raised against a 20 kDa Arabidopsis thaliana cyclophilin, revealed the induction of a cross-reacting band (97 kDa, BsCyp) in both embryos and endosperm in a time dependent manner. The BsCyp boiling-stable polypeptide was also regulated by exogenous application of ABA, indicating its role in water stress adaptation. Drought stress also resulted in a significant induction of boiling-stable protein (BsCyp) in both embryos and endosperm. Based upon these observations the possible role of BsCyp in water stress tolerance is discussed.
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Acknowledgements
We are also grateful to Prof. C.S. Gasser, Section of Molecular and Cellular Biology, Division of Biological Sciences, University of California, Davis, CA for the gift of Arabidopsis thaliana 20 kDa cyclophilin antiserum. This research was supported by the management committee, Lyallpur Khalsa College, Jallandhar, Punjab, India.
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Sharma, A.D., Vasudeva, R. & Kaur, R. Expression of a boiling-stable protein (BsCyp) in response to heat shock, drought and ABA treatments in Sorghum bicolor . Plant Growth Regul 50, 249–254 (2006). https://doi.org/10.1007/s10725-006-9134-2
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DOI: https://doi.org/10.1007/s10725-006-9134-2