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The involvement of hydrogen peroxide in UV-B-inhibited pollen germination and tube growth of Paeonia suffruticosa and Paulownia tomentosa in vitro

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Abstract

Previous studies have shown that UV-B could affect pollen germination and tube growth. However, the mechanism of response of pollen to UV-B has not been clear. The purpose of this study was to investigate the role of hydrogen peroxide (H2O2) in the UV-B-induced reduction of in vitro pollen germination and tube growth of Paeonia suffruticosa Andr. and Paulownia tomentosa Steud. Exposure of pollen of the two species to 0.4 and 0.8 W m−2 UV-B radiation for 3 h resulted in not only the reduction of pollen germination and tube growth, but also the H2O2 production in pollen grain and tube. Also, exogenous H2O2 inhibited pollen germination and tube growth of the two species in a dose-dependence manner. Two scavengers of H2O2, ascorbic acid and catalase, largely prevented not only the H2O2 generation, but also the reduction of pollen germination and tube growth induced by UV-B radiation in the two species. These results indicate that H2O2 is involved in the UV-B-inhibited pollen germination and tube growth.

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Authors and Affiliations

  1. School of Life Sciences, Shaanxi Normal University, Xi’an, 710062, P.R. China

    Jun-Min He, Zhi-Hui Liu, Han Xu & Xiao-Ping She

  2. Medical School, Xi’an Jiaotong University, Xi’an, 710061, P.R. China

    Chen Huang

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Correspondence to Jun-Min He.

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He, JM., Liu, ZH., Xu, H. et al. The involvement of hydrogen peroxide in UV-B-inhibited pollen germination and tube growth of Paeonia suffruticosa and Paulownia tomentosa in vitro. Plant Growth Regul 49, 199–208 (2006). https://doi.org/10.1007/s10725-006-9105-7

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