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Nickel-induced Inhibition of Wheat Root Growth is Related to H2O2 Production, but not to Lipid Peroxidation

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Abstract

Effects of exogenous nickel (Ni: 10 and 200 µM) on growth, mitotic activity, Ni accumulation, H2O2 content and lipid peroxidation as well as the activities of various antioxidative enzymes, such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione peroxidase (GSH-Px) were investigated in wheat roots. A considerable Ni accumulation in the roots occurred at both the concentrations. Although Ni at 10 µM did not have any significant effect on root growth, it strongly inhibited the root growth at 200 µM. Mitotic activity in the root tips was not significantly affected by exposure of the seedlings to 10 µM Ni; however, it was almost completely inhibited at 200 µM treatment. Ni stress did not result in any significant changes in CAT and APX activities as well as lipid peroxidation. However, H2O2 concentration increased up to 82% over the control in the roots of seedlings exposed to 200 µM Ni. There was a significant decline in both SOD (50%) and GSH-Px (20–30%) activities in the roots when the seedlings were treated with 200 µM Ni. The results indicated that a strong inhibition of wheat root growth caused by Ni stress was not due to enhanced lipid peroxidation, but might be related to the accumulation of H2O2 in root tissue.

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Abbreviations

APX:

Ascorbate peroxidase

CAT:

Catalase

GSH:

Reduced glutathione

GSH-Px:

Glutathione peroxidase

MBTH:

3-Methyl-2-benzothiazolinone hydrazone

MI:

Mitotic index

NBT:

Nitro blue tetrazolium

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

TBARS:

Thiobarbituric acid reactive substances

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Authors and Affiliations

  1. Department of Plant Physiology and Biochemistry, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland

    E. Gajewska, R. Andrzejewska & M. Skłodowska

  2. Department of Industrial Microbiology and Biotechnology, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland

    M. Słaba

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  1. E. Gajewska
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  2. M. Słaba
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  3. R. Andrzejewska
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  4. M. Skłodowska
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Correspondence to E. Gajewska.

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Gajewska, E., Słaba, M., Andrzejewska, R. et al. Nickel-induced Inhibition of Wheat Root Growth is Related to H2O2 Production, but not to Lipid Peroxidation. Plant Growth Regul 49, 95–103 (2006). https://doi.org/10.1007/s10725-006-0018-2

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  • DOI: https://doi.org/10.1007/s10725-006-0018-2

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