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Characterization of Strigolactones, Germination Stimulants for the Root Parasitic Plants Striga and Orobanche, Produced by Maize, Millet and Sorghum

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Abstract

The germination stimulants for root parasitic plants Striga and Orobanche produced by sorghum (Sorghum bicolor (L.) Moench), maize (Zea mays L.), and pearl millet (Pennisetum typhoideum Rich.) were examined. Characterization of strigolactones in the root exudates from the plants grown hydroponically was conducted by comparing retention times of germination stimulants on reverse phase high performance liquid chromatography (HPLC) with those of synthetic standards, and by using HPLC linked with tandem mass spectrometry (LC/MS/MS). All the plants tested, except for a sorghum cultivar Swarna, were found to exude two major stimulants, 5-deoxy-strigol, which is known as a branching factor for arbuscular mycorrhizal (AM) fungi, and an isomer of strigol, tentatively named sorghumol. Swarna was found to exude 5-deoxy-strigol and strigol. These results imply that 5-deoxy-strigol is one of major germination stimulants of gramineous plants and that major stimulants may differ even among cultivars within the same species.

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Abbreviations

EtOH:

ethanol

EtOAc:

ethyl acetate

HPLC:

high-performance liquid chromatography

LC/MS/MS:

liquid chromatography-tandem mass spectrometry

MeOH:

methanol

MRM:

multiple-reaction monitoring

Nloss:

neutral loss

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Authors and Affiliations

  1. Center for Research on Wild Plants, Utsunomiya University, 350 Mine-machi, Utsunomiya, 321-8505, Japan

    Ayman A. Awad, Daisuke Sato, Dai Kusumoto, Hiroaki Kamioka, Yasutomo Takeuchi & Koichi Yoneyama

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  1. Ayman A. Awad
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  2. Daisuke Sato
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  3. Dai Kusumoto
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  4. Hiroaki Kamioka
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  5. Yasutomo Takeuchi
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  6. Koichi Yoneyama
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Correspondence to Koichi Yoneyama.

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Awad, A.A., Sato, D., Kusumoto, D. et al. Characterization of Strigolactones, Germination Stimulants for the Root Parasitic Plants Striga and Orobanche, Produced by Maize, Millet and Sorghum. Plant Growth Regul 48, 221–227 (2006). https://doi.org/10.1007/s10725-006-0009-3

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  • DOI: https://doi.org/10.1007/s10725-006-0009-3

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