Plant Growth Regulation

, Volume 48, Issue 2, pp 111–117 | Cite as

Effect of Auxins on Adventitious Root Development from Single Node Cuttings of Camellia sinensis (L.) Kuntze and Associated Biochemical Changes

  • Gyana Ranjan Rout


An attempt was made to induce rooting from single node cuttings of Camellia sinensis var. TV-20 under controlled conditions and study its biochemical changes during rooting. The nodal cuttings were pretreated with different concentrations of IAA, NAA and IBA and kept in a growth chamber (25 ±2 °C, 16 h photoperiod (55 μ mol m−2 s−1) with cool, white fluorescent lamps and 65% relative humidity) for 12 h. Among the three auxins used for pretreatment, IBA showed more positive response on rooting as compared to IAA and NAA within 2 weeks of transfer to potting medium. Among four concentrations of IBA tested, 75 ppm gave maximum percentage of rooting, number of roots and root length. Therefore, IBA was used further in experiments for biochemical investigation. The adventitious rooting was obtained in three distinct phases i.e. induction (0–12 days), initiation (12–14 days) and expression (14–18 days). IAA-oxidase activity of IBA-treated cuttings increased slightly as compared to control. The activity was found to decrease during induction and initiation phases and increase during expression phase. The peroxidase activity in IBA-treated cuttings increased up to initiation phase and declined at the expression phase. Polyphenoloxidase activity increased both in IBA-treated and control cuttings during induction and initiation phase but declined slowly during expression phase. Total phenolic content was higher in IBA-treated cuttings, particularly in initiation and expression phases and it also correlated with peroxidase activity. Phenolics might be playing key role for induction of adventitious rooting, and phenolic compounds can be used as rooting enhancer in tea plant.


Auxins Camellia sinensis Enzyme activity Nodal cutting Propagation 



indole-3-acetic acid


indole-3-butyric acid


1-naphthaleneacetic acid


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  1. Anonymous1976The Wealth of IndiaCouncil of Scientific Industrial ResearchNew DelhiGoogle Scholar
  2. Aeschbacher, R.A., Schiefelbein, J.W., Benfey, P.N. 1994The genetic and molecular basis of root developmentAnnu. Rev. Plant Physiol. Plant Mol. Biol.452545CrossRefGoogle Scholar
  3. Banerjee, M., Agarwal, B. 1990In vitro rooting teaCamellia sinensis (L.) O.KuntzeInd. J. Expt. Biol.28936939Google Scholar
  4. Bradford, M.M. 1976A rapid and sensitive method for the quantification of microgram quanties of protein utilizing the principle of protein-dye bindingAnal. Biochem.72248254CrossRefPubMedGoogle Scholar
  5. Beffa, R., Martin, H.V., Pilet, P.E. 1990In vitro oxidation of indoleacetic acid by soluble auxin-oxidases and peroxidases from maize rootsPlant Physiol.94485491Google Scholar
  6. Berthon, J.Y., Battraw, M.J., Gaspar, T., Boyer, N. 1993Early test using phenolic compounds and peroxidase activity to improve in vitro rooting of Sequoiadendron giganteum (Lindl.). BuchholzSaussurea277Google Scholar
  7. Bray, H.G., Thorpe, W.V. 1954Analysis of phenolic compounds of interest in metabolismGlick, D. eds. Methods of Biochemical AnalysisIntersci Publ.New York2752Google Scholar
  8. Davis, T.D., Haissig, B.E, Sankhla, N. 1988Adventitious root formation in cuttingsDudley, T.R. eds. Advances in Plant Sciences SeriesDioscorides PressPortlandOregon128142Google Scholar
  9. DeKlerk, G.J. 1996Marker of adventitious root formationAgronomie16609616Google Scholar
  10. DeKlerk, G.J., Krieken, W.V.D., Jong, J.C. 1999The formation of adventitious roots: new concepts, new possibilitiesIn Vitro Cell Dev. Biol.-Plant.35189199Google Scholar
  11. Fett-Neto, A.G., Teixeira, S.L., Dasilva, E.A.M., Sant Annna, R. 1992Biochemical and morphological changes during in vitro rhizogenesis in cuttings of Sequoia sempervirens (D. Don) EndlJ. Plant Physiol.140720728Google Scholar
  12. Gaspar, T., Kevers, C., Hausman, J.F., Ripetti, V. 1994Peroxidase activity and endogenous free auxin during adventitious root formationLumsden, P.J.Nicholas, J.R.Davis, W.J. eds. Physiology, Growth and Development of Plants in CultureKluwer Acad. Publ.Dordrecht, The NetherlandsGoogle Scholar
  13. Gaspar, T., Kevers, C., Hausman, J.F. 1997Indissociable chief factors in the inductive phase of adventitious rootingAltman, A.Waisel, M. eds. Biology of Root Formation and DevelopmentPlenum PressNew YorkGoogle Scholar
  14. Gunasekare, M.T.K, Evans, P.K. 2000In vitro rooting of microshoots of tea (Camellia sinensis L.)SriLanka J. Tea Sci.66515Google Scholar
  15. Haissig, B.E. 1974Influence of auxin and auxin synergists on adventitious root primordium initiation and developmentN. Z. J. Sci.4299310Google Scholar
  16. Hartmann H.T., Kester D.E. and Davies F.T. 1993. Plant propagation-principles and practices. 5th ed. Prentice Hall of India Pvt. Ltd, pp. 199–255.Google Scholar
  17. Hausman, J.F. 1993Changes in peroxidase activity, auxin level and ethylene production during root formation by poplar shoots raised in vitroPlant Growth Regul.13263268CrossRefGoogle Scholar
  18. Hausman, J.F., Evers, D., Kevers, C., Gaspar, T. 1997Internal controls of root induction in poplar shoots raised in vitroAngew. Bot.71104107Google Scholar
  19. Hess C.E. 1962. Characterization of the rooting cofactors extracted from Hedera helix L. and Hibiscus rosa-sinensis L. Proc. 16th Int. Hort. Cong., pp. 382–388.Google Scholar
  20. Kar, M., Mishra, D. 1976Catalaseperoxidase and polyphenol oxidase activities during rice leaf senescencePlant Physiol.57315319Google Scholar
  21. Kevers, C., Hausman, J.F., Fairre-Rampant, O., Evers, D., Gaspar, T. 1997Hormonal control of adventitious rooting: progress and questionsJ. Appl. Bot.717179Google Scholar
  22. Leung, A.Y. 1980Encyclopedia of Common Natural Ingredients Used in Food, Drugs, and CosmeticsJohn Wiley and sonNew YorkGoogle Scholar
  23. Liu, J.H., Reid, D.M. 1992Adventitious rooting in hypocotyls of sunflower (Helianthus annuus) seedlings. IV. The role of changes in endogenous free and conjugated indole-3-acetic acidPhysiol. Plant.86285292CrossRefGoogle Scholar
  24. Liu, Z.H., Hsiao, I.C., Pan, Y.W. 1996Effect of naphthaleneacetic acid on endogenous indole-3-acetic acidPeroxidase and auxin oxidase in hypocotyls cuttings of soybean during root formationBot. Bull. Acad. Sin.37247253Google Scholar
  25. Mato, M.C., Rua, M.L., Ferro, E. 1988Changes in levels of peroxidases and phenolics during root formation in Vitis cultured in vitroPhysiol. Plant.728488Google Scholar
  26. Moncousin, C.H., Gaspar, T. 1983Peroxidase as a marker for rooting improvement in Cynara scolymus L. cultivated in vitroBiochem. Physiol. Pflanzen178263271Google Scholar
  27. Murashige, T., Skoog, F. 1962A revised medium for rapid growth and bioassay with tobacco tissue culturesPhysiol. Plant.15473497Google Scholar
  28. Nag, S., Saha, K., Choudhuri, M.A. 2001Role of auxin and polyamines in adventitious root formation in relation to changes in compounds involved in rootingJ. Plant Growth Regul.20182194CrossRefGoogle Scholar
  29. Nordstrom, A.C., Eliasson, J. 1991Levels of endogenous indole-3-acetic acid and indole-3-acetylaspartic acid during adventitious root formation in pea cuttingsPhysiol. Plant.82599605CrossRefGoogle Scholar
  30. Sato, Y., Sugiyama, M., Gorecki, R.J., Fukuda, H., Komamine, A. 1993Interrelationship between lignin deposition and the activities of peroxidase isozymes in differentiating tracheary elements of ZinniaPlanta.189584589CrossRefGoogle Scholar
  31. Sharma, M., Sood, A., Nagar, P.K., Prakash, O.M., Ahuja, P.S. 1999Direct rooting and hardening of tea microshoots in the fieldPlant Cell. Tiss. Org. Cult.58111118CrossRefGoogle Scholar
  32. Wiesmann, Z., Riov, J., Epstein, E. 1988Comparison of movement and metabolism of indole-3-acetic acid and indole-3-butyric acid in mung bean cuttingsPhysiol. Plant.74556560Google Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  1. 1.Plant Biotechnology DivisionRegional Plant Resource CentreBhubaneswarIndia

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