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Antagonistic Effects of Triazolo[4,5-d]pyrimidine and Pyridylurea Derivatives on Cytokinin-Induced Cytokinin Oxidase/Dehydrogenase Activity in Young Pea Plants

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Abstract

The effect of strong and weak cytokinin antagonists, belonging to the groups of triazolo[4,5-d]pyrimidines (TP), and pyridyl-phenylurea derivatives (PU), on cytokinin oxidase/dehydrogenase activity (CKX) in the tissues of young pea plants was studied. Tested anticytokinins, with the exception of the most efficient one – PU-1, were able to promote increased CKX activity in roots, when applied alone, but they had no significant influence on the enzymatic activity in leaves. N6-benzyladenine (BA) and 1-(2-chloropyridin-4-yl)-3-phenylurea (CPPU) provoked strong increase in CKX activity in roots, while in leaves considerable inhibition of enzymatic activity was observed. Different types of anticytokinins exhibited diverse preference towards taking off the action of purine and phenylurea cytokinins over CKX activity.

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Abbreviations

BA:

N6-benzyladenine

CKX:

cytokinin oxidase/dehydrogenase activity

CPPU:

1-(2-chloropyridin-4-yl)-3-phenylurea

PU-1:

3-[(pyridin-2-yl)methyl]-1-phenylurea

PU-9:

3-[(pyridin-4-yl) methyl]-1-phenylurea)

TP-7:

tert.butyl 2-(3-benzyl-3H-[1,2,3]-triazolo[4,5-d]pyrimidin-7-ylamino)acetate

TP-8:

2-(3-benzyl-3H-[1,2,3]-triazolo[4,5-d]pyrimidin-7-ylamino)acetohydrazide

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Authors and Affiliations

  1. Acad. M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Bl. 21, 1113, Sofia, Bulgaria

    Irina Vaseva-Gemisheva, Iskren Sergiev, Desislava Todorova, Vera Alexieva & Emanuil Karanov

  2. Faculty of Chemistry, St. Kliment Ohridski University of Sofia, 1, J. Boucher Blvd, 1126, Sofia, Bulgaria

    Elena Stanoeva

  3. Faculty of Ecology, Landscape Architecture and Agronomy, University of Forestry, 10 Kl. Ohridsky Blvd, 1756, Sofia, Bulgaria

    Viktoria Lachkova

Authors
  1. Irina Vaseva-Gemisheva
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  2. Iskren Sergiev
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  3. Desislava Todorova
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  4. Vera Alexieva
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  5. Elena Stanoeva
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  6. Viktoria Lachkova
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  7. Emanuil Karanov
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Correspondence to Irina Vaseva-Gemisheva.

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Vaseva-Gemisheva, I., Sergiev, I., Todorova, D. et al. Antagonistic Effects of Triazolo[4,5-d]pyrimidine and Pyridylurea Derivatives on Cytokinin-Induced Cytokinin Oxidase/Dehydrogenase Activity in Young Pea Plants. Plant Growth Regul 46, 193–197 (2005). https://doi.org/10.1007/s10725-005-0650-2

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