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Insights into morphological and molecular diversity in grasspea (Lathyrus sativus L.) germplasm

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Abstract

Grasspea (Lathyrus sativus L.) is a multifaceted orphan legume considered a climate-smart crop because of its wider adaptation, higher yield, and better nutritional value. The present study involved morpho-molecular characterization and identification of the best-performing accessions for important yield contributing traits like days to 80% maturity (BANG31, BANG38), number of pods per plant (IFLA276, IC489623) and 100-seed weight (IFLA1439, IC0634674). Traits like plant height, petiole length, and 100-seed weight showed high heritability coupled with high genetic advance as a percentage of the mean (GAM). Principal component analysis gave five principal components, and hierarchical clustering divided the accessions into four groups. Twenty simple sequence repeat markers showed polymorphism with polymorphic information content ranging from 0.02 to 0.67 with an average of 0.34 and heterozygosity ranging between 0 to 0.5. Molecular diversity research by principal coordinate analysis and hierarchical clustering led to similar grouping patterns as noted for morpho-molecular dendrograms for 100-seed weight and days to maturity. The identified superior and diverse accessions can be used for grasspea improvement to bring back this golden legume to large-scale cultivation.

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References

  • Akter L, Mahbub M, Habib MA, Alam SS (2015) Characterization of three varieties of Lathyrus sativus L. by fluorescent karyotype and RAPD analysis. Cytologia 80:457–465

    Article  CAS  Google Scholar 

  • Allkin R (1986) Names and Synonyms of Species and Subspecies in the’Vicieae’: issue 3. University of Southampton, Biology Department, Vicieae Database Project

    Google Scholar 

  • Arora RK, Mathur PN, Riley KW, Adham Y (1996) Lathyrus Genetic Resources in Asia. In: Proceedings of a Regional Workshop, 27–29 December 1995, Indira Gandhi Agricultural University, Raipur, India. IPGRI Office for South Asia, New Delhi

  • Arora RK, Mathur PN, Riley KW, Adham Y (1996) Lathyrus genetic resources in asia. In: Proceedings of a Regional Workshop, 27–29 December 1995. Raipur: Indira Gandhi Agricultural University; New Delhi: IPGRI Office for South Asia

  • Barpete S (2015) Genetic associations, variability and diversity in biochemical and morphological seed characters in Indian grass pea (Lathyrus sativus L.) accessions. Fresen Environ Bull 24:492–497

    CAS  Google Scholar 

  • Barpete S, Gupta P, Singh M, Kumar S (2020) Culture selected somaclonal variants showing low-ODAP and high protein content in nineteen grass pea (Lathyrus sativus L.) genotypes. Plant Cell. Tissue and Organ Culture (PCTOC) 142:625–634

    Article  CAS  Google Scholar 

  • Burton GW, Devane DE (1953) Estimating heritability in tall fescue (Festuca arundinacea) from replicated clonal material 1. Agron J 45(10):478–481

    Article  Google Scholar 

  • Campbell CG (1997) Grass pea, Lathyrus sativus L. Bioversity International

  • Emmrich PM, Sarkar A, Njaci I, Kaithakottil GG, Ellis N, Moore C, Edwards A, Heavens D, Waite D, Cheema J, Trick M (2020) A draft genome of grass pea (Lathyrus sativus), a resilient diploid legume. BioRxiv 2020–04

  • Falconer DS (1996) Introduction to quantitative genetics. Pearson Education India

  • Federer WF (1956) Experimental design 81:4, p 334. LWW

  • Federer WT (1961) Augmented designs with one-way elimination of heterogeneity. Bio 17(3):447–473

    Google Scholar 

  • Gixhari B, Doko A, Hobdari V, Vrapi H (2016) Diversity of grass pea (L. sativum) landraces for sustainable field grass pea breeding in Albania. Int J Ecosyst Ecol Sci 6(1)

  • Gore PG, Gupta V, Singh R, Tripathi K, Kumar R, Kumari G, Madhavan L, Dikshit HK, Venkateswaran K, Pandey A, Singh N (2022) Insights into the genetic diversity of an underutilized Indian legume, Vigna stipulacea (Lam.) Kuntz., using morphological traits and microsatellite markers. Plos one 17(1): e0262634

  • Govindaraj M, Shanmugasundaram P, Muthiah AR (2010) Estimates of genetic parameters for yield and yield attributes in elite lines and popular cultivars of India’s pearl millet. Afr J Agric Res. 5(22):3060–4

  • Grela ER, Rybiński W, Klebaniuk R, Matras J (2010) Morphological characteristics of some accessions of grass pea (Lathyrus sativus L.) grown in Europe and nutritional traits of their seeds. Genet Resour Crop Evol 57:693–701

    Article  Google Scholar 

  • Grela ER, Rybiński W, Matras J, Sobolewska S (2012) Variability of phenotypic and morphological characteristics of some Lathyrus sativus L. and Lathyrus cicera L. accessions and nutritional traits of their seeds. Genet Resour Crop Evol 59:1687–1703

    Article  Google Scholar 

  • Jackson MT, Yunus AG (1984) Variation in the grass pea (Lathyrus sativus L.) and wild species. Euphytica 33:549–559

    Article  Google Scholar 

  • Jeberson MS, Gonmei R, Kumar M, Shashidhar KS, Singh NB, Sharma PR (2018) Genetic variability, heritability, correlation coefficient and path analysis in Lathyrus for yield and its related contributes under NEH condition. J Pharmacogn Phytochem 7(6):1806–1809

    Google Scholar 

  • Johnson HW, Robinson HF, Comstock RE (1995) Estimates of genetic and environmental variability in soybeans 1. Agron J 47(7):314–318

    Article  Google Scholar 

  • Kislev ME (1989) Origins of the cultivation of Lathyrus sativus and L. cicera (Fabaceae). Economic Botany 43(2), 262–270

  • Kumar S, Dubey DK (2001) Variability, heritability and correlation studies in grasspea (Lathyrus sativus L.). Lathyrus Lathyrism Newsletter 2:79–81

    CAS  Google Scholar 

  • Kumari V, Prasad R (2005) Model plant type in Khesari (Lathyrus sativus L.) suitable for hill farming. Lathyrus Lathyrism Newsletter 4:15–17

    Google Scholar 

  • Lambein F, Travella S, Kuo YH, Van Montagu M, Heijde M (2019) Grass pea (Lathyrus sativus L.): orphan crop, nutraceutical or just plain food? Planta 250:821–838

    Article  CAS  PubMed  Google Scholar 

  • Lioi L, Sparvoli F, Sonnante G, Laghetti G, Lupo F, Zaccardelli M (2011) Characterization of Italian grasspea (Lathyrus sativus L.) germplasm using agronomic traits, biochemical and molecular markers. Genet Resour Crop Evol 58:425–437

    Article  CAS  Google Scholar 

  • Mahapatra NS, Das A, Bhattacharyya P, Bhattacharya S, Pal S, Barpete S (2020) Studies on genetic variability, divergence and association of characters in grass pea. J Crop Weed 16(1):155–161

    Article  Google Scholar 

  • Mekonen DA, Abraham A, Oselebe H, Afiukwa C, Ilesanmi O, Abebe TD (2022) Genetic diversity and population structure analysis of Grass pea (Lathyrus sativus L.) accessions collected from North-Western Ethiopia using SSR markers. Genetic Resources and Crop Evolution 69(3):1247–60

  • Mondini L, Noorani A, Pagnotta MA (2009) Assessing plant genetic diversity by molecular tools. Diversity 1(1):19–35

    Article  CAS  Google Scholar 

  • Patto MV, Skiba B, Pang EC, Ochatt SJ, Lambein F, Rubiales D (2006) Lathyrus improvement for resistance against biotic and abiotic stresses: from classical breeding to marker assisted selection. Euphytica 147:133–147

    Article  Google Scholar 

  • Piergiovanni AR, Damascelli A (2011) L-Homoarginine accumulation in grass pea (Lathyrus sativus L.) dry seeds. A preliminary survey. Food Nutr Sci 2(3):207

  • Plitmann U, Gabay R, Cohen O (1995) Innovations in the tribe Vicieae (Fabaceae) from Israel. Isr J Plant Sci 43(3):249–258

    Article  Google Scholar 

  • Polignano GB, Uggenti P, Alba V, Bisignano V, Della Gatta C (2005) Morpho-agronomic diversity in grasspea (Lathyrus sativus L.). Plant Genet Res 3(1):29–34

  • Rahman MM, Kumar J, Rahman MA, Afzal MA (1995) Natural outcrossing in Lathyrus sativus L. Indian J Genet Pl Br 55(02):204–207

    Google Scholar 

  • Ramya KR, Gupta R, Mishra M, Pandey A, Tripathi K, Bhatt KC, Singh R (2023) A modified DNA isolation protocol for high-quality DNA and long-term storability in grasspea (Lathyrus sativus L.). Indian J Genet Pl Br 83(04):602–4.

  • Ramya KR, Tripathi K, Pandey A, Barpete S, Gore PG, Raina AP, Khawar KM, Swain N, Sarker A (2022) Rediscovering the potential of multifaceted orphan legume Grasspea-a sustainable resource with high nutritional values. Front Nutr 8:826208

    Article  PubMed  PubMed Central  Google Scholar 

  • Saraswat KS (1980) The ancient remains of the crop plants at Atranjikhera (c. 2000–1500 BC). J Indian Bot Soc 59(4):306–19

  • Sidorova KK, Glyanenko MN, Mishchenko TM, Vlasova EY, Shumny VK (2015) Symbiotic nitrogen fixation in legumes as a genetic and selection trait. Vavilov J Gent Br 19(1):50–57

    Article  Google Scholar 

  • Singh PK, Sadhukhan R, Kumar A (2017) Correlation studied on several quantitative traits in induced mutagenic population of grasspea (Lathyrus sativus L.). Int J Curr Microbiol App Sci 6(10):612–9

  • Singh RS, Sood BC, Kumar N (2012) Identification of yield attributes for selection through correlation and path analysis of lentil (Lens culinaris Medik.). Crop Research 43(1to3):171–7

  • Smartt J (1984) Evolution of grain legumes. I Mediterr Pul Exp Agric 20(4):275–296

    Article  Google Scholar 

  • SN International (2019) Genstat for Windows, 19th Edn. Available online at: Genstat.co.uk (accessed march 20, 2023)

  • Talukdar D (2009) Association of seed yield components along with seed neurotoxin content in different varieties and induced mutant lines of grass pea (Lathyrus sativus L.). Int J Plant Sci (Muzaffarnagar) 4(2):378–80

  • Tripathi K, Gore PG, Ramya KR, Sarker A (2022) Grass Pea an inherent abiotic Stress-Tolerant legume: current status and future scope under changing environment. In: Developing Climate Resilient Grain and Forage Legumes pp. 125–139. Singapore: Springer Nature Singapore

  • Tripathi K, Sadhukhan R, Das A, Jana K, Semwal DP, Kumar A, Sarker A, Ahlawat SP (2022) Khesari (Lathyrus sativus L.), an ancient legume for future gain: An expedition collection from parts of West Bengal state of Eastern India. Indian J Tradit Knowl (IJTK) 21(2):395–403

  • Tyagi SD, Khan MH (2011) Correlation, path-coefficient and genetic diversity in lentil (Lens culinaris Medik) under rainfed conditions. Int Res J Plant Sci 2(7):191–200

    Google Scholar 

  • Wang F, Yang T, Burlyaeva M, Li L, Jiang J, Fang L, Redden R, Zong X (2015) Genetic diversity of grasspea and its relative species revealed by SSR markers. PLoS ONE 10(3):e0118542

    Article  PubMed  PubMed Central  Google Scholar 

  • Yang T, Jiang J, Burlyaeva M, Hu J, Coyne CJ, Kumar S, Redden R, Sun X, Wang F, Chang J, Hao X (2014) Large-scale microsatellite development in grasspea (Lathyrus sativus L.), an orphan legume of the arid areas. BMC Plant Biol 14(1):1–2

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Acknowledgements

Authors acknowledge the Director, ICAR-NBPGR, New Delhi; The Director and the Dean, The graduate school ICAR-IARI New Delhi.

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R.K.R.and K.T. Performed the experiments, recording data, data analysis and Writing-original draft; A.P. and K.T.Conceptualization; K.T, P.G. and R.S. Review and Editing; N.R. Data analysis and infographics; R.S.Molecular lab facility, Data Analysis; R.K. and R.R.assisted in Data recording; S.B., S.G, P.GGermplasm resources; K.C. Supervision, Review and Editing

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Correspondence to Kuldeep Tripathi or Rakesh Singh.

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KR, R., Tripathi, K., Singh, R. et al. Insights into morphological and molecular diversity in grasspea (Lathyrus sativus L.) germplasm. Genet Resour Crop Evol (2024). https://doi.org/10.1007/s10722-024-01992-7

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