Abstract
This study was conducted to determine the protein, fat, mineral, fatty acid, and bioactive compound contents of 47 walnut hybrids obtained by intraspecies hybridization of G1, G2, and G3 walnut genotypes with Chandler, Maraş 18, and Pedro walnut cultivars. A wide variation was found between the hybrid walnut genotypes regarding the investigated properties. Among the hybrid genotypes, C1 had the highest bioactive compound content in terms of total flavonoids with 147.63 mg 100 g−1, P4 had the highest antioxidant activity with 72.73 mmol 100 g−1, and M13 had the highest total phenolic content with 1465.87 mg 100 g−1. In addition, the highest oleic acid content was 23.23%, and polyunsaturated fatty acids (MUFA) content was 23.4%. The M15 had the highest protein content (18.06%) and total fat content (68.3%). Genotype P6 showed the highest results in terms of K (764.26 mg 100 g−1), P (764.26 mg 100 g−1), Fe (2.61 mg 100 g−1), PUFA (74.11%) and linoleic acid (63.74%). Similarly, the P15 genotype was found to be significant for fatty acids, with the highest values for myristric acid (0.07%), eicosenoic acid (1.164%), and eicosadienoic acid (0.069%). According to the principal component analysis, the eigenvalue for 10 principal components was above 1. It corresponded to 82.5% of the total variation, thus effectively revealing the variation among walnut genotypes for the investigated characteristics. These results indicate that most of the examined walnut genotypes are rich in minerals, fatty acids, and bioactive compounds. Therefore, these walnut hybrids with high nutritional value and health-important traits can be used as genetic material in walnut breeding programs and to develop new walnut cultivars.
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References
AOAC (2010) Official methods of analysis of association of official analytical chemists. 18th edn. Washington, DC
Akbari V, Jamei R, Heidari R, Esfahlan AJ (2012) Antiradical activity of different parts of walnut (Juglans regia L.) fruit as a function of genotype. Food Chem 135(4):2404–2410
Ara T, Shafi S, Ghazwani M, Mir JI, Shah AH, Qadri RA, Hakami AR, Khalid M, Hani U, Wahab S (2023) In vitro potent anticancer, antifungal, and antioxidant efficacy of Walnut (Juglans regia L.) Genotypes. Agronomy 13:1232
Aryapak S, Ziarati P (2014) Nutritive value of persian walnut (Juglans regia L.). Orchards Am Eurasian J Agric Environ Sci 14(11):1228–1235
Balta MF, Uçar L, Karakaya O (2016) Çorum ili Merkez ilçe ümitvar ceviz genotipleri: Fiziksel ve kimyasal özellikler. Bahçe, Özel sayı 771–776
Bernard A, Barreneche T, Lheureux F, Dirlewanger E (2018) Analysis of genetic diversity and structure in a worldwide walnut (Juglans regia L.) germplasm using SSR markers. PLoS ONE 13(11):e0208021. https://doi.org/10.1371/journal.pone.0208021
Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature 181:1199–1200
Bremner JM (1996) Nitrogen-Total. In: Methods of Soil Analysis: Part 3 Chemical Methods Sparks DL, Page AL, Helmke PA, Loeppert RH, Soltanpour PN, Tabatabai MA, Johnston CT, Sumner ME (eds) SSSA Book Series 5: 1085–1121
Bujdoso G, Vegvari G, Hajnal V, Ficzek G, Toth M (2014) Phenolic profile of the kernel of selected persian walnut (Juglans regia L.) cultivars. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 42(1):24–29
Caspermeyer J (2019) Scientists Crack Origin of the Persian Walnut. Mol Biol Evol 36(11):2630. https://doi.org/10.1093/molbev/msz164
Chang CC, Yang MH, Wen HM, Chern JC (2002) Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 10(3):178–182
Cosmulescu S, Botu M, Achim G, Baciu A, Gruia M, Trandafir I (2014) Polyphenol content in walnut (Juglans regia L.) mature leaves. Acta Hortic 1050:205–212
Cosmulescu S, Trandafir I, Nour V, Botu M (2015) Total phenolic, flavonoid distribution and antioxidant capacity in skin, pulp and fruit extracts of plum cultivars. J Food Biochem 39:64–69. https://doi.org/10.1111/jfbc.12112
Demirağ ÜM (2019) F1 melez ceviz populasyonunda yağ asidi ve tokoferol içeriklerinin belirlenmesi (Msc. Thesis). Çukurova Üniversitesi Fen Bilimleri Enstitüsü, Adana
de Souza RM, Mathias BM, da Silveira CLP, Aucelio RQ (2005) Inductively coupled plasma optical emission spectrometry for trace multi-element determination in vegetable oils, margarine and butter after stabilization with propan-1-ol and water. Spectrochim Acta B 60:711–715
Dieffenbacher A, Pocklington WD (1992) IUPAC Standart methods for the analysis of oils, Fats and derivatives. Fifth Edition Method II.D.19, 6th edn. Pergamon Press, Oxford, pp 96–102
Erdogan U, Argin S, Turan M, Cakmakci R, Olgun M (2021) Biochemical and bioactive content in fruits of Walnut (Juglans Regia L.) genotypes from Turkey. Fresenius Environ Bull 30:6713–6727
Esen BE (2013) Health related properties of different parts of walnut (Juglans Regia L.) and a walnut drink (Msc. Thesis). Istanbul Technical University Institute of Science, Istanbul
FAOSTAT—Food and Agricultural Organization of the United Nations. Walnuts—production statistics in 2021. fao.org (Date accessed: 25.09.2023)
Feng X, Zhou H, Zulfiqar S, Luo X, Hu Y, Feng L, Malvolti ME, Woeste K, Zhao P (2018) The Phytogeographic History of Common Walnut in China. Front Plant Sci 9:1399. https://doi.org/10.3389/fpls.2018.01399
Fjellstrom RG, Parfitt DE (1994) Walnut (Juglans spp.) genetic diversity determined by restriction fragment length polymorphisms. Genome 37:690–700. https://doi.org/10.1139/g94-097
Gülsoy E, Pehluvan M, Şimşek M (2019) Determination of fatty acids, α-tocopherol, β-caroten, minerals, and some pomological properties of walnut genotypes selected from Aras Valley (Eastern Turkey). Iran J Chem Chem Eng 38(3):211–221
Gündeşli MA, Korkmaz N, Okatan V (2019) Polyphenol content and antioxidant capacity of berries: a review. Int J Agric For Life Sci 3(2):350–361
Jahanban Esfahlan A, Amarowicz R (2018) Walnut (Juglans regia L.) shell pyroligneous acid: chemical constituents and functional applications. RSC Adv 8(40):22376–22391
James CS (1995) Analytical Chemistry of Foods, 1st edn. Chapman and Hall, London
Jan R, Ara T, Mir JI (2022) Quality profiling and estimation of total phenols, flavonoids, flavonols and antioxidative potential of walnut kernel (Juglans regia) from Kashmir Valley. J Nuts 13:211–225
Ji F, Ma Q, Zhang W et al (2021) A genome variation map provides insights into the genetics of walnut adaptation and agronomic traits. Genome Biol. https://doi.org/10.1186/s13059-021-02517-6
Juranović Cindrić I, Zeiner M, Hlebec D (2018) Mineral composition of elements in Walnuts and Walnut oils. Int J Environ Res Public Health 15:2674. https://doi.org/10.3390/ijerph15122674
Kafkas E, Attar SH, Gundesli MA, Ozcan A, Ergun M (2020) Phenolic and fatty acid profile, and protein content of different walnut cultivars and genotypes (Juglans regia L.) grown in the USA. Int J Fruit Sci 20(53):1711–1720
Kafkas E, Burgut A, Ozcan H, Ozcan A, Sutyemez M, Kafkas S, Türemis N (2017) Fatty acid, total phenol and tocopherol profiles of some walnut cultivars: a comparative study. Food Nutr Sci 8(12):1074–1084. https://doi.org/10.4236/fns.2017.812079
Kırbaşlar FG, Türker G, Özsoy-Güneş Z, Ünal M, Dülger B, Ertaş E, Kızılkaya B (2012) Evaluation of fatty acid composition, antioxidant and antimicrobial activity, mineral composition and calories values of some nuts and seeds from Turkey. Rec Nat Prod 6:339–349
Li Q, Yin R, Zhang QR, Wang XP, Hu XJ, Gao ZD, Duan ZM (2017) Chemometrics analysis on the content of fatty acid compositions in different walnut (Juglans regia L.) varieties. Eur Food Res Technol 243:2235–2242
Martínez ML, Labuckas DO, Lamarque AL, Maestri DM (2010) Walnut (Juglans regia L.): genetic resources, chemistry, by-products. J Sci Food Agric 90(12):1959–1967. https://doi.org/10.1002/jsfa.4059
McGranahan G, Leslie C, In JN, Moore JR, Ballington, et al (1990) Walnuts (Juglans) genetic resources of temperate fruit and nut crops. Int Soc Horticul Sci. https://doi.org/10.17660/ActaHortic.1991.290.20
Mertens D (2005a) Plants preparation of laboratory sample. In: Official methods of analysis 18th Chap. 3, Horwitz W, Latimer GW (ed) Gaitherburg, Maryland USA pp 1–2
Mertens D (2005b) Metal in plants and pet foods. In: Official methods of analysis 18th Chap. 3, Horwitz W, Latimer GW (ed) Gaitherburg, Maryland USA pp 3–4
Muradoglu F, Oguz HI, Yildiz K, Yilmaz H (2010) Some chemical composition of walnut (Juglans regia L.) selections from Eastern Turkey. Afr J Agric Res 5:2379–2385
Nunes PX, Silva SF, Guedes RJ, Almeida S (2012) Biological oxidations and antioxidant activity of natural products. In: Venketeshwer R (ed) Phytochemicals as nutraceuticals. Global approaches to their role in nutrition and health pp 1–20.
Poggetti L, Ferfuia C, Chiaba C, Testolin R, Baldini M (2018) Kernel oil content and oil composition in walnut (Juglans regia L.) accessions from north eastern Italy. J Sci Food Agric 98:955–962
Rabrenovic B, Dimic E, Maksimovic M, Sobajic S, Gajic-Krstajic L (2011) Determination of fatty acid and tocopherol compositions and the oxidative stability of walnut (Juglans regia L.) cultivars grown in Serbia. Czech J Food Sci 29(1):74–78
Santos A, Barros L, Calhelha RC et al (2013) Leaves and decoction of Juglans regia L.: different performances regarding bioactive compounds and in vitro antioxidant and antitumor effects. Ind Crops Prod 51:430–436
Shen D, Wu S, Zheng Y, Han Y, Ni Z, Li S, Liu Y (2021) Characterization of iron walnut in different regions of China based on phytochemical composition. J Food Sci Technol 58:1358–1367
Sheorey SD, Sengupta R, Hinge MA (2011) Heart healthy nuts. Int J Curr Pharm Rev Res 2(3):145–160
Tapia MI, Sánchez-Morgado JR, García-Parra J, Ramírez R, Hernández T, González-Gómez D (2013) Comparative study of the nutritional and bioactive compounds content of four walnut (Juglans regia L.) cultivars. J Food Compos Anal 31(2):232–237
Trandafir I, Cosmulescu S, Botu M, Nour V (2016) Antioxidant activity, and phenolic and mineral contents of the walnut kernel (Juglans regia L.) as a function of the pellicle color. Fruits 71(3):177–184
Uzun A, Kaplan M, Pinar H, Paris K (2021) Oil contents and fatty acid composition of walnut genotypes selected from Central Anatolia region and assessments through GT biplot analysis. Bul Chem Commun 53(3):279–286
Vahdati K, Arab MM, Sarikhani S, Sadat-Hosseini M, Leslie CA, Brown PJ (2019) Advances in Persian Walnut (Juglans regia L.) breeding strategies. In: Al-Khayri J, Jain S, Johnson D (eds) Advances in plant breeding strategies: nut and Beverage crops. Springer, Cham. https://doi.org/10.1007/978-3-030-23112-5_11
Wang R, Zhong D, Wu S, Han Y, Zheng Y et al (2020) The phytochemical profiles for walnuts (J. Regia and J. Sigillata) from China with protected geographical indications. Food Sci Technol 41:695–701
Wei F, Li Y, Sun D, Chen Q, Fu M, Zhao H, Chen X, Huang Y, Xu H (2022) Odor, tastes, nutritional compounds and antioxidant activity of fresh-eating walnut during ripening. Sci Hortic 293:110744
Yan S, Wang X, Yang C, Wang J, Wang Y, Wu B, Mohammad P, Zheng J (2021) Insights into walnut lipid metabolism from metabolome and transcriptome analysis. Front Genet. https://doi.org/10.3389/fgene.2021.715731
Yerlikaya C, Yucel S, Erturk U, Korukluoglu M (2012) Proximate composition, minerals and fatty acid composition of Juglans regia L. genotypes and cultivars grown in Turkey. Braz Arch Biol Technol 55:677–683
Yildiz E, Pinar H, Uzun A, Yaman M, Sumbul A, Ercisli S (2021) Identification of genetic diversity among Juglans regia L. genotypes using molecular, morphological, and fatty acid data. Genet Resour Crop Evol 68:1425–1437
Yılmaz S, Akça Y (2017) Determination of biochemical properties and fatty acid composition of new walnut (Juglans regia) genotypes. J Agric Fac Gaziosmanpasa Univ 34(2):74–80
Yılmaz M, Karakaya O, Balta MF, Balta F, Yaman İ (2019) Çakıldak fındık çeşidinde iç meyve iriliğine bağlı olarak biyokimyasal özelliklerin değişimi. Akad Zir Derg 8:61–70
Zheng Y, Wu S, Wang R, Wu Y, Zhang W, Han Y, Liu Y (2020) Analysis and correlationship of chemical components of various walnut (Juglans regia L.) cultivars. J Food Meas Charact 14:3605–3614
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Kömür, Y.K., Karakaya, O., Sütyemez, M. et al. Characterization of walnut (Juglans regia L.) hybrid genotypes; fatty acid composition, biochemical properties and nutrient contents. Genet Resour Crop Evol (2023). https://doi.org/10.1007/s10722-023-01810-6
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DOI: https://doi.org/10.1007/s10722-023-01810-6