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Relevance of genetic and active ingredient content differences in Leonurus japonicus Houtt from different origins

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Abstract

Leonurus japonicus Houtt. (Lamiaceae) is a perennial herb, which is commonly used in the treatment of cardiovascular, uterine, and gynecological diseases. In the present study, we constructed a phylogenetic tree based on the ITS + psbA-trnH + rbcL + rpoB concatenation sequence and performed partial least squares-discriminant analysis was used high-performance liquid chromatography. The results indicated that the concatenation sequence could distinguish among L. japonicus from different origins. Additionally, chemical analysis revealed intergroup differences, albeit of lower quality than the molecular phylogeny. By combining both methods, we were able to correlate the genetic and chemical differences among L. japonicus derived from different origins. Furthermore, our combined chemical and phylogenetic analyses suggested that differences in metabolites are not solely influenced by genetic differences but also environmental factors. These findings can be valuable for the artificial cultivation of L. japonicus and provide new insights for improving its quality.

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Acknowledgements

This work was supported by the Sichuan Science and Technology Project (Grant No. 2022JDTD0029); National Natural Science Foundation of China (Grant No. U19A2010).

Funding

This work was supported by the Sichuan Science and Technology Project (Grant No. 2022JDTD0029); National Natural Science Foundation of China (Grant No. U19A2010).

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Authors and Affiliations

  1. State Key Laboratory of Southwestern Chinese Medicine Resources, College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, Sichuan, China

    Ying Han, Jiawei Wen, Chao Chen, Tingting Zhang, Wanqing Feng, Ziwei Zhao & Yang He

  2. National Institute of Measurement and Testing Technology, Chengdu, 610021, Sichuan, China

    Lihua Zhou

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  1. Ying Han
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  2. Jiawei Wen
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  3. Chao Chen
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  4. Lihua Zhou
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Correspondence to Yang He.

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Han, Y., Wen, J., Chen, C. et al. Relevance of genetic and active ingredient content differences in Leonurus japonicus Houtt from different origins. Genet Resour Crop Evol 71, 801–810 (2024). https://doi.org/10.1007/s10722-023-01660-2

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