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Assessment and suitability of DUS traits to screen potato (Solanum tuberosum L.) genotypes for Phytophthora spp. infection resistance by using phenotypic and molecular (SSR) markers

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Abstract

The present investigation aimed to identify reliable morphological markers that could serve as indicators for Phytophthora infestans Mont. resistance in potato germplasm. A total of 86 potato genotypes were subjected to genetic diversity assessment using simple sequence repeats, followed by a field trial alongside to evaluate resistance responses and morphological characteristics. Two genotypes (LA CHIPPER and J/95–144) exhibited high resistance to late blight (LB), while four genotypes (BR 6609–7, NO–NOVA, MP/99–406, and CP–4174) displayed moderate resistance. The disease response was juxtaposed DUS characters, thus leading to the identification of critical traits (semi-compact to compact foliage, solid stem, angular stem cross-section, no secondary stem coloration, anthocyanin pigmentation on the stem, smooth tuber skin, and white to creamy tuber flesh coloration). These DUS parameters could be utilized for developing high-yielding, LB-resistant potato cultivars in future breeding programs.

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Abbreviations

ANOVA:

Analysis of Variance

AUDPC:

Area Under the Disease Progress Curve

CAN:

Canada

CIP:

International Potato Center

COL:

Colorado’s country

CPRI:

Central Potato Research Institute, Shimla

DMRT:

Duncan's Multiple Range Test

DNA:

Deoxyribonucleic Acid

DUS:

Distinctness, Uniformity and Stability

FRA:

France

GDR:

German Democratic Republic

GDR:

German Democratic Republic Deutshe Demokratische Republik

GFR:

Germany

ICAR:

Indian Council of Agricultural Research

IND:

India

NET:

Netherlands

KOR:

Korea

LB:

Late Blight

MCMC:

Markov chain Monte Carlo

NTSYS–PC:

Numerical Taxonomy and Multivariate Analysis System

PCoA:

Principal Coordinate Analysis

PCR:

Polymerase Chain Reaction

PER:

Peru

PPV & FRA:

Protection of Plant Varieties and Farmers' Rights Authority

RHS:

Royal Horticulture Society

SAHN:

Sequential agglomerative hierarchical non-overlapping

SSR:

Simple Sequence Repeat

UK:

United Kingdom

UPOV:

The International Union for the Protection of New Varieties of Plants

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Acknowledgements

This research study received support from the MS Swaminathan School of Agriculture, Shoolini University, India, and the Department of Seed Science and Technology, Dr. YSP UHF, Nauni, Solan, India. The authors gratefully acknowledge the technical assistance and conducive research environment provided by the Department of Seed Science and Technology, Dr. YSP UHF, Nauni, Solan, and ICAR-Central Potato Research Institute, Shimla, India.

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Authors and Affiliations

  1. Department of Seed Science and Technology, Dr YSP UHF, Nauni, Solan, Himachal Pradesh, 173230, India

    Vinaykumar Rachappanavar, Manish Kumar & Satish K. Gupta

  2. MS Swaminathan School of Agriculture, Shoolini University of Biotechnology and Management Sciences, Solan, Himachal Pradesh, 173212, India

    Vinaykumar Rachappanavar

  3. Department of Crop Improvement, ICAR-Central Potato Research Institute, Shimla, 1730001, India

    Vinod Kumar &  Dalamu

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Rachappanavar, V., Kumar, M., Kumar, V. et al. Assessment and suitability of DUS traits to screen potato (Solanum tuberosum L.) genotypes for Phytophthora spp. infection resistance by using phenotypic and molecular (SSR) markers. Genet Resour Crop Evol 71, 199–223 (2024). https://doi.org/10.1007/s10722-023-01616-6

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