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Bioinformatics and gene expression analysis of the legume F-box/WD40 proteins in NaCl and high temperature stress

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Abstract

In this study we aimed to analyze legume F-box/WD40 protein sequences bioinformatically and to test their gene expression profiles during NaCl and high temperature stress. We examined the molecular responses to these stressors for genes encoding subunits of the Skp, Cullin, F-box (SCF) complex in beans, chickpeas, and barrel medic, including the F-box domain and WD40 repeats. High temperature and salinity stresses were applied to in vitro grown legumes. Two conserved F-box/WD40 proteins were identified in each genome and named MET30 and WD40/YVTN. We examined their gene expression profiles under NaCl and high temperature stress to determine their response and potential functions in response to abiotic stresses. A computational prediction of their 3-dimensional structures suggests that they are conserved among legumes. Our results show that the legume MET30 and WD40/YVTN proteins may have direct roles in the response to high temperature and NaCl stresses. We conclude that the legume proteins MET30 and WD40/YVTN may have a role in the Cdc34/SCF complex based on sequence similarities and expression profiles. Also, according to their response to abiotic stresses, our results suggest that these proteins play an active role in responding to NaCl and high temperature.

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The data generated and analyzed are available from the corresponding authors on reasonable request.

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Acknowledgements

The authors gratefully acknowledge financial support from the Scientific Research Projects Coordination Unit of Istanbul University under project number 36473.

Funding

This research was funded by the Scientific Research Projects Coordination Unit of Istanbul University with Project (Grant) Number 36473.

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HÇ: conceptualization, methodology, software, investigation, writing—original draft, visualization, data curation, formal analysis, validation. NTK: conceptualization, methodology, resources, writing—review and editing, supervision, funding acquisition, project administration, formal analysis. AA: software, investigation, writing—review and editing, data curation.

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Correspondence to Neslihan Turgut Kara.

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Çelik, H., Aravena, A. & Turgut Kara, N. Bioinformatics and gene expression analysis of the legume F-box/WD40 proteins in NaCl and high temperature stress. Genet Resour Crop Evol 70, 2637–2655 (2023). https://doi.org/10.1007/s10722-023-01592-x

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