Abstract
DNA variation studies were undertaken on the Pi-ta gene of wild, weedy and cultivated rice as a contribution to genetic resources management, despite this gene having no direct utility in current breeding programs. In this study, samples of wild rice (Oryza rufipogon), weedy rice (O. sativa f. spontanea), and cultivar KDML105 were used to explore DNA variation of the leucine-rich domain in exon2 and the entire DNA sequence of the Pi-ta gene. All rice samples examined contained only pi-ta susceptible allele, indicated by codon TCT which encodes the amino acid serine (Ser) at position 918 of pi-ta protein. In addition, wild rice population from Laos contained a synonymous SNPs (CAA/CAG, encodes Glutamine) found in exon2. Overall, the entire Pi-ta gene sequence showed very low DNA variation in both coding and non-coding region of the gene. A total of ten SNPs were observed, five SNPs in coding sequence had synonymous substitution suggesting that weak selective forces act on pi-ta protein in populations of wild, weedy and KDML105.
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Acknowledgements
This research was financially supported by Thailand Science Research and Innovation (TSRI). The author would like to thank V. Pilab for molecular laboratory assistant researcher. The author also thanks Dr. Adrian Plant at Mahasarakham University for helpful English improvement and review of this manuscript.
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Prathepha, P. Low level of DNA variation in blast resistance Pi-ta gene of wild, weedy rice and Thai jasmine rice Khao Dawk Mali 105. Genet Resour Crop Evol 70, 115–123 (2023). https://doi.org/10.1007/s10722-022-01505-4
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DOI: https://doi.org/10.1007/s10722-022-01505-4