Skip to main content
SpringerLink
Log in

Validation of resistance to cereal cyst nematode (Heterodera avenae) and yield performance study in doubled haploid lines of wheat (Triticum aestivum L.)

  • Short Communication
  • Published:
Genetic Resources and Crop Evolution Aims and scope Submit manuscript

Abstract

Cereal cyst nematode (CCN), Heterodera avenae, is an important plant parasitic nematode in major wheat growing states of northern India. Recently, we detected a major QTL, “QCcn.ha-2D” using 114 doubled haploid (DH) lines of wheat obtained from a cross between CIMMYT synthetic hexaploid wheat line W7984 (also known as M6) and the hard red spring wheat Opata M85. In the present study, resistance nature of selected DH lines, harboring this major QTL, was validated for H. avenae resistance under controlled and natural conditions. Different developmental stages of nematode were also studied through staining of infected resistant and susceptible plant roots. In contrast to susceptible DH line roots, staining of resistant DH line roots showed decrease in total number of juveniles, J3s with absence of J4 stage juveniles. In comparison to known resistant parent, most of the resistant DH lines showed better biological yield per plot, grain yield per plot and harvest index, measured over two years. Present study indicates that selected DH lines are promising prebreeding material to introduce novel genetic variation for H. avenae resistance in cultivated wheat.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Data availability

Related data is available as supplementary data.

References

  • Bishnoi SP, Bajaj HK (2004) On the species and pathotypes of Heterodera avenae complex of India. Indian J Nematol 34(2):147–152

    Google Scholar 

  • Bybd DW Jr, Kirkpatrick T, Barker K (1983) An improved technique for clearing and staining plant tissues for detection of nematodes. J Nematol 15(1):142–143

    CAS  Google Scholar 

  • Cobb NA (1918) Estimating the nema population of soil, with special reference to the sugar-beet and root-gall nemas, Heterodera schachtii Schmidt and Heterodera radicicola (Greef) Muller: and with a description of Tylencholaimus aequalis n. sp (Vol. 1). US Government Printing Office

  • Cui J, Peng H, Huang W, Liu S, Wu D, Kong L, He W, Peng D (2017) Phenotype and cellular response of wheat lines carrying Cre genes to Heterodera avenae pathotype Ha91. Plant Dis 101(11):1885–1894. https://doi.org/10.1094/PDIS-03-17-0404-RE

    Article  CAS  Google Scholar 

  • Dababat A, Arif MAR, Toktay H, Atiya O, Shokat S, Gul E, Imren M, Singh S (2021) A GWAS to identify the cereal cyst nematode (Heterodera filipjevi) resistance loci in diverse wheat prebreeding lines. J Appl Gene 62(1):93–98. https://doi.org/10.1007/s13353-020-00607-y

    Article  CAS  Google Scholar 

  • Dunckel SM, Olson EL, Rouse MN, Bowden RL, Poland JA (2015) Genetic mapping of race- specific stem rust resistance in the synthetic hexaploid W7984× Opata M85 mapping population. Crop Sci 5(6):2580–2588. https://doi.org/10.2135/cropsci2014.11.0755

    Article  CAS  Google Scholar 

  • Eastwood RF, Lagudah ES, Appels R (1994) A directed search for DNA sequences tightly linked to cereal cyst nematode resistance genes in Triticum tauschii. Genome 37(2):311–319. https://doi.org/10.1139/g94-043

    Article  CAS  Google Scholar 

  • Eastwood RF, Lagudah ES, Appels R, Hannah M, Kollmorgen JF (1991) Triticum tauschii: a novel source of resistance to cereal cyst nematode (Heterodera avenae). Aust J Agric Res 42:69–77. https://doi.org/10.1071/AR9910069

    Article  Google Scholar 

  • Fisher JM (1982) Towards a consistent laboratory assay for resistance to Heterodera avenae1. EPPO Bulletin 12(4):445–449. https://doi.org/10.1111/j.1365-2338.1982.tb01828.x

    Article  Google Scholar 

  • Kong LA, Wu DQ, Huang WK, Peng H, Wang GF, Cui JK, Liu SM, Li ZG, Yang J, Peng DL (2015) Large-scale identification of wheat genes resistant to cereal cyst nematode Heterodera avenae using comparative transcriptomic analysis. BMC Genomics 16(1):1–18. https://doi.org/10.1186/s12864-015-2037-8

    Article  CAS  Google Scholar 

  • Kumar D, Sharma S, Sharma R, Pundir S, Singh VK, Chaturvedi D, Singh B, Kumar S, Sharma S (2021) Genome-wide association study in hexaploid wheat identifies novel genomic regions associated with resistance to root lesion nematode (Pratylenchus thornei). Sci Rep 11(1):1–14. https://doi.org/10.1038/s41598-021-80996-0

    Article  CAS  Google Scholar 

  • Martin EM, Eastwood RF, Ogbonnaya FC (2004) Identification of microsatellite markers associated with the cereal cyst nematode resistance gene Cre3 in wheat. Aust J Agric Res 55(12):1205–1211. https://doi.org/10.1071/AR04085

    Article  CAS  Google Scholar 

  • Mokrini F, Viaene N, Lieven Waeyenberge L (2017) Characterization of cereal cyst nematodes (Heterodera spp.) in Morocco based on morphology, morphometrics and rDNA-ITS sequence analysis. J Plant Protec Res 57(3):219–227

    Article  CAS  Google Scholar 

  • Pankaj SC, Rohatgi D, Tomar SM, Sirohi A, Gaur HS, Kishor V, Bishnoi SP, Kumar R, Yadav SM (2008) Genetics of resistance in wheat against the cereal cyst nematode, Heterodera avenae Woll. Nematol Mediterr 36:19–23

    Google Scholar 

  • Pariyar SR, Dababat AA, Siddique S, Erginbas-Orakci G, Elashry A, Morgounov A, Grundler FM (2016) Identification and characterization of resistance to the cereal cyst nematode Heterodera filipjevi in winter wheat. Nematology 18(4):377–402. https://doi.org/10.1163/15685411-00002964

    Article  Google Scholar 

  • Plamenov D, Spetsov P (2011) Synthetic hexaploid lines are valuable resources for biotic stress resistance in wheat improvement. J Plant Pathol 93(2):251–262

    Google Scholar 

  • Premachandran D, Von Mende N, Hussey RS, McClure MA (1988) A method for staining nematode secretions and structures. J Nematol 20(1):70–78

    CAS  Google Scholar 

  • Pundir S, Sharma R., Kumar D, Singh VK, Chaturvedi D, Kanwar RS, Roder MS, Borner A, Ganal MW, Gupta PK, Sharma S, Sharma S (2022) QTL mapping for resistance against cereal cyst nematode (Heterodera avenae Woll.) in wheat (Triticum aestivum L.). Sci Rep 12:9586. https://doi.org/10.1038/s41598-022-12988-7

  • Puza V, Chundelova D, Nermut J, Zurovcova M, Mracek Z (2015) Intra-individual variability of ITS regions in entomopathogenic nematodes (Steinernematidae: Nematoda): implications for their taxonomy. Biocontrol 60(4):547–554. https://doi.org/10.1007/s10526-015-9664-5

    Article  CAS  Google Scholar 

  • Seid A, İmren M, Ali MA, Toumi F, Paulitz T, Dababat AA (2021) Genetic resistance of wheat towards plant-parasitic nematodes: current status and future prospects. Biotech Stud 30:43–62. https://doi.org/10.38042/biotechstudies.944678

    Article  Google Scholar 

  • Sharma SN, Sain RS, Singh H (2004) Breeding for resistance to wheat nematodes (Heterodera avenae). Acta Agron Hung 52(2):189–197

    Article  Google Scholar 

  • Smiley RW, Nicol JM (2009) Nematodes which challenge global wheat production. In: Carver BF (ed) Wheat Science and Trade. Wiley-Blackwell, Ames, pp 171–187

  • Subbotin SA, Mundo-Ocampo M, Baldwin JG (2010) Systematics of Cyst Nematodes (Nematoda: Heteroderinae), Part B. Brill.

  • Toumi F, Waeyenberge L, Viaene N, Dababat A, Nicol JM, Ogbonnaya F, Moens M (2013) Development of two species-specific primer sets to detect the cereal cyst nematodes Heterodera avenae and Heterodera filipjevi. Eur J Plant Pathol 136(3):613–624. https://doi.org/10.1007/s10658-013-0192-9

    Article  CAS  Google Scholar 

  • Yadav N, Dhanda SS, Kanwar RS, Singh S (2016) Inheritance of resistance for Cereal Cyst Nematode (Heterodera avenae Woll) in Wheat (Triticum aestivum L). Int J Agric Sci 8(56):3047–3049

    Google Scholar 

  • Yan GP, Smiley RW, Okubara PA, Easley SA, Sheedy JG, Thompson AL (2008) Molecular diagnostics of root-lesion nematodes and cereal cyst nematodes. Oregon Agric Exp Station Spec Rep 1083:93–108

    Google Scholar 

Download references

Acknowledgements

Thanks are due to the Department of Biotechnology (DBT), Govt. of India for providing funds in the form of Ramalingaswami Re-entry fellowship project (BT/RLF/Re-entry/34/2015; award BT/HRD/35/02/2006) to Shailendra Sharma to carry out this research work. Authors are highly thankful to Marion S  Röder (IPK, Gatersleben, Germany) and Martin W Ganal (Trait Genetics GmbH, Gatersleben, Germany) for providing seed material of DH mapping population. Authors are also thankful to Ch. Charan Singh University, Meerut for providing various research facilities.

Funding

This work was supported by Department of Biotechnology, Govt. of India. Research project (BT/RLF/Re-entry/34/2015; award BT/HRD/35/02/2006) was awarded to Shailendra Sharma.

Author information

Authors and Affiliations

  1. Department of Genetics and Plant Breeding, Chaudhary Charan Singh University (CCSU), Uttar Pradesh, Meerut, 250 004, India

    Saksham Pundir, Vikas Kumar Singh, Satish Kumar, Deepti Chaturvedi, Deepak Kumar, Shiveta Sharma & Shailendra Sharma

  2. Department of Botany, Chaudhary Charan Singh University (CCSU), Uttar Pradesh, Meerut, 250 004, India

    Saksham Pundir, Deepak Kumar & Ashok Kumar

  3. Department of Nematology, Chaudhary Charan Singh Haryana Agricultural University (CCSHAU), Hisar, 125 004, Haryana, India

    Rambir Singh Kanwar

  4. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrassen 3, 06466, Gatersleben, Germany

    Andreas Börner

Authors
  1. Saksham Pundir
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Vikas Kumar Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Satish Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Deepti Chaturvedi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Deepak Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Rambir Singh Kanwar
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ashok Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Andreas Börner
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Shiveta Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Shailendra Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

SS conceived the research idea. SS, SP, VKS, SSh, DC, DK and SK performed experiments and also conducted analysis. All authors including RSK, AB and AK contributed to the writing of the manuscript. The manuscript was read and revised by all authors and finalized by SS.

Corresponding author

Correspondence to Shailendra Sharma.

Ethics declarations

Conflict of interest

Authors declare no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 43 KB)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Pundir, S., Singh, V.K., Kumar, S. et al. Validation of resistance to cereal cyst nematode (Heterodera avenae) and yield performance study in doubled haploid lines of wheat (Triticum aestivum L.). Genet Resour Crop Evol 70, 107–113 (2023). https://doi.org/10.1007/s10722-022-01495-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10722-022-01495-3

Keywords

Search

Navigation