Skip to main content
SpringerLink
Log in

Biosystematics relationships among Marrubium L. (Lamiaceae) species in Iran

  • Research Article
  • Published:
Genetic Resources and Crop Evolution Aims and scope Submit manuscript

Abstract

Marrubium L., is a problematic genus of Lamiaceae family with approximately 40 taxa that some of its species grow in Iran. In the current study, we studied infrageneric taxonomic relationships among six Iranian Marrubium species using morphological and anatomical traits, and molecular genetic techniques, including inter simple sequence repeats (ISSR), chloroplast DNA (cpDNA) and nuclear ribosomal internal transcribed spacer (ITS). We investigated five individuals per each species. Most of the studied qualitative and quantitative morphological characteristics differed significantly (P ≤ 0.001) among these species, and we found five morphotype groups in the PCA plot of morphological traits. Leaf blade anatomical structure was dorsi-ventral in all species. Meanwhile, we observed a significant variation in most of the studied anatomical variables. Based on anatomical features, these species were clustered into three groups. Results of ISSR study revealed that the total genetic diversity at the species level (Ht) was higher than within each species (Hs). These findings were supported by the results of AMOVA test, Gst and Nm values. According to STRUCTURE analysis and Nei's genetic distance, these species were divided into 5 genotype groups. The studied species were clustered separately in cpDNA and ITS trees of phylogenetic studies. We observed a high level of infraspecific divergence in the evaluated species. In addition, the employed taxonomical markers have enough potential to separate the investigated species. However, the clustering patterns of the species based on the examined techniques were not the same, except those for M. parviflorum Fisch. & C.A. Mey., and M. crassidens Bioss., that were clustered closely in most cases. It can be concluded that the evaluated species revealed a taxonomically complex group resulting from high infraspecific variation, polyploidy and hybridization.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Data availability

Not applicable.

Code availability

Not applicable.

References

  • Abu-Asab MS, Cantino PD (1994) Systematic implications of pollen morphology in subfamilies Lamioideae and Pogostemonoideae. Ann Mo Bot Gard 81(1):653–686

    Article  Google Scholar 

  • Akgül G, Ketenoglu O, Pinar NM, Kurt L (2008) Pollen and seed morphology of the genus Marrubium (Lamiaceae) in Turkey. Ann Bot Fenn 45(1):1–10

    Article  Google Scholar 

  • Almeida VR, Costa AF, Mantovani A, Gonçalves-Esteves V, Arruda RCO, Forzza RC (2009) Morphological phylogenetics of Quesnelia (Bromeliaceae, Bromelioideae). Syst Bot 34:660–672

    Article  Google Scholar 

  • Aoyama EM, Sajo MG (2003) Leaf structure of Aechmea Ruiz & Pav. subgenus Lamprococcus (Beer) Baker and related species (Bromeliaceae). Rev Bras Bot 26:461–473. https://doi.org/10.1590/S0100-84042003000400005

    Article  Google Scholar 

  • Arnold ML (2015) Divergence with genetic exchange. Oxford University Press, Oxford

    Book  Google Scholar 

  • Asaf S, Khan AL, Khan A, Al-Harrasi A (2020) Unraveling the chloroplast genomes of two Prosopis species to identify its genomic information, comparative analyses and phylogenetic relationship. Int J Mol Sci 21(9):3280. https://doi.org/10.3390/ijms21093280

    Article  CAS  PubMed Central  Google Scholar 

  • Ayaz A, Zaman W, Ullah F, Saqib S, Jamshed S, Bahadur S, Shakoor A, Arshad B (2020b) Systematics study through scanning electron microscopy; a tool for the authentication of herbal drug Mentha suaveolens Ehrh. Microsc Res Tech 83(1):81–87. https://doi.org/10.1002/jemt.23391

    Article  CAS  PubMed  Google Scholar 

  • Ayaz A, Zaman W, Saqib S, Ullah F, Mahmood T (2020) Phylogeny and diversity of Lamiaceae based on rps14 gene in Pakistan. Genetika 52(2):435–452

    Article  Google Scholar 

  • Bentham G (1848) Labiatae. In: De Candolle AP (ed) Prodromus systematics naturalis regni vegetabilis. Treuttel and Würtz, Paris, pp 536–549

    Google Scholar 

  • Boissier PE (1879) Flora orientalis. Regnum Academic Scientific, Basel

    Google Scholar 

  • Briquet J (1896) Labiatae. In: Engler A, Pran HK (eds) Die naturlichen pflanzenfamilien. W. Engelmann, Leipzig, pp 183–375

    Google Scholar 

  • Cullen J (1972) Marrubium L. In: Tutin TG, Heywood VH, Burges NA, Valentine DH (eds) Flora Europaea. Cambridge University Press, Cambridge, pp 137–138

  • Cullen J (1982) Marrubium L. In: Davis PH (ed) Flora of Turkey and the Aegean Islands. Edinburgh University Press, Edinburgh, pp 165–178

  • Culley TMM, Wallace LE, Gengler-Nowak KM, Crawford DJ (2002) A comparison of two methods of calculating GST, a genetic measure of population differentiation. Am J Bot 89:460–465

    Article  Google Scholar 

  • Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13

    Google Scholar 

  • Drummond AJ, Rambaut A (2007) BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evol Biol 7:214. https://doi.org/10.1186/1471-2148-7-214

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Drummond AJ, Suchard MA, Xie D, Rambaut A (2012) Bayesian phylogenetics with BEAUti and the BEAST 1.7. Mol Biol Evol 29(8):1969–1973. https://doi.org/10.1093/molbev/mss075

  • El Bardai S, Morel S, Wibo N, Faber M, Llabers NG, Lyoussi B et al (2003) The vasorlaxant activity of murrabenol and marrubin from M. vulgare. Planta Med 69(1):75–77

    Article  Google Scholar 

  • Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software structure: a simulation study. Mol Ecol 14(8):2611–2620. https://doi.org/10.1111/j.1365-294X.2005.02553.x

    Article  CAS  PubMed  Google Scholar 

  • Freeland JR, Kirk H, Peterson SD (2011) Molecular ecology, 2nd edn. Wiley-Blackwell, London

    Book  Google Scholar 

  • Hedge IC (1992) A global survey of the biogeography of the Labiatae. In: Harley RM, Reynolds T (eds) Advances in labiate science. Royal Botanic Gardens, Kew, pp 7–17

  • Hu H, Hu Q, Al-Shehbaz IA, Luo X, Zeng T, Guo X, Liu J (2016) Species delimitation and interspecific relationships of the genus Orychophragmus (Brassicaceae) inferred from whole chloroplast genomes. Front Plant Sci 7:1826. https://doi.org/10.3389/fpls.2016.01826

    Article  PubMed  PubMed Central  Google Scholar 

  • Jamzad Z (2012) Lamiaceae. In: Assadi M, Masoumi AA, Mozafarian V (eds) Flora of Iran. Research Institute of Forest and Rangelands, Tehran, pp 152–251

    Google Scholar 

  • Jehanzeb S, Zafar M, Ahmad M, Sultana S, Zaman W, Ullah F (2020) Comparative petioler anatomy of tribe Mentheae subfamily Nepetoideae, Lamiaceae from Pakistan. Feddes Repert 131:163–174. https://doi.org/10.1002/fedr.202000009

    Article  Google Scholar 

  • Johansen DA (1940) Plant microtechnique. McGraw, New York

    Google Scholar 

  • Jost L (2008) GST and its relatives do not measure differentiation. Mol Ecol 17:4015–4026. https://doi.org/10.1111/j.1365-294X.2008.03887.x

    Article  PubMed  Google Scholar 

  • Kharazian N, Hashemi M (2017) Chemotaxonomy and morphological studies in five Marrubium L. species in Iran. Iran J Sci Technol Trans Sci. https://doi.org/10.1007/s40995-017-0202-4

    Article  Google Scholar 

  • Koohdar F, Sheidai M, Talebi SM, Noormohammadi Z, Ghasemzadeh-Baraki S (2016) Genetic diversity, population structure and morphological variability in the Lallemantia royleana (Lamiaceae) from Iran. Phytol Balc 22(1):29–38

    Google Scholar 

  • Martin E, Çetin Ö, Akgül G, Hilal AY (2011) New chromosome numbers in the genus Marrubium (Horehound) from Turkey. Biol Divers Conserv 4(2):185–188

    Google Scholar 

  • McCauley DE, Sundby AK, Bailey MF, Welch ME (2007) Inheritance of chloroplast DNA is not strictly maternal in Silene vulgaris (Caryophyllaceae): evidence from experimental crosses and natural populations. Am J Bot 94:1333–1337. https://doi.org/10.3732/ajb.94.8.1333

    Article  CAS  PubMed  Google Scholar 

  • Meirmans PG (2012) AMOVA–based clustering of population genetic data. J Hered 103(5):744–750. https://doi.org/10.1093/jhered/ess047

    Article  PubMed  Google Scholar 

  • Moon HK, Smets E, Huysmans S (2010) Phylogeny of tribe Mentheae (Lamiaceae): the story of molecules and micromorphological characters. Taxon 59(4):1065–1076

    Article  Google Scholar 

  • Pagnotta MA (2018) Comparison among methods and statistical software packages to analyze germplasm genetic diversity by means of codominant markers. J Multidiscip Res 1:197–215

    Google Scholar 

  • Paton AJ, Springate D, Suddee S, Otieno D, Grayer RJ, Harley MM, Willis F, Simmonds MS, Powell MP, Savolainen V (2004) Phylogeny and evolution of basils and allies (Ocimeae, Labiatae) based on the three plastid DNA regions. Mol Phylogenet Evol 31:277–299

    Article  CAS  Google Scholar 

  • Pinho C, Hey J (2010) Divergence with gene flow: models and data. Annu Rev Ecol Evol Syst 41:215–230. https://doi.org/10.1146/annurev-ecolsys-102209-144644

    Article  Google Scholar 

  • Podani J (2000) Introduction to the exploration of multivariate biological data. Backhuys, Leiden

  • Proença SL, Sajo MG (2004) Leaf anatomy of Aechmea Ruiz & Pav. (Bromeliaceae) of São Paulo State. Brazil Acta Bot Bras 18:319–331. https://doi.org/10.1590/S0102-33062004000200011

    Article  Google Scholar 

  • Rambaut A (2010) FigTree v1.3.1. Institute of Evolutionary Biology, University of Edinburgh, Edinburgh. http://tree.bio.ed.ac.uk/software/figtree/

  • Robert CE (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32(5):1792–1797. https://doi.org/10.1093/nar/gkh340

    Article  CAS  Google Scholar 

  • Salehi N, Kharazian N, Shiran B (2018) Genetic diversity of Marrubium species from Zagrous region (Iran), using inter simple sequence repeat molecular marker. J Sci, Islamic Repub Iran 29(1):7–19

    Google Scholar 

  • Seybold S (1982) Marrubium L. In: Rechinger KH (ed) Flora Iranica. Akademische Druck- und Verlagsanstalt, Graz, Austria, pp 88–108

  • Shah SN, Ahmad M, Zafar M, Ullah F, Zaman W, Mazumdar J, Khuram I, Khan SM (2019) Leaf micromorphological adaptations of resurrection ferns in Northern Pakistan. Flora 255:1–10. https://doi.org/10.1016/j.flora.2019.03.018

    Article  Google Scholar 

  • Sheidai M, Taban F, Talebi SM, Noormohammadi Z (2016) Genetic and morphological diversity in Stachys lavandulifolia (Lamiaceae) populations. Biologija 62:9–24

    Article  CAS  Google Scholar 

  • Stankovic MS (2011) Total phenolic content, flavonoid concentration and antioxidant activity of Marrubium peregrinum L. extracts. Kragujev J Sci 33:63–72

    Google Scholar 

  • Tabaripour R, Sheidai M, Talebi SM, Noormohammadi Z (2018) Genetic divergence and speciation within Ziziphora capitata (Lamiaceae): molecular and micromorphological evidence. Biodiversitas 19:667–675. https://doi.org/10.13057/biodiv/d190250

    Article  Google Scholar 

  • Talebi SM, Matsyura AV (2020) Genetic variability and population structure of some Iranian Salvia limbata C. A. Mey populations. Ecol Montenegrina 29:56–65

  • Talebi SM, Sheidai M, Arianejad F, Matsyura A (2019) Stem anatomical study of some Iranian Marrubium L. species. Biodiversitas 20:2589–2595. https://doi.org/10.1357/biodiv/d200922

    Article  Google Scholar 

  • Talebi SM, Askary M, Khalili N, Matsyura A, Ghorbanpour M, Kariman K (2021) Genetic structure and essential oil composition in wild populations of Salvia multicaulis Vahl. Biochem Syst Ecol 96:104269

    Article  CAS  Google Scholar 

  • Talebi SM, Tabaripour R, Matsyura A (2021) Genetic diversity and population structure of diverse Iranian Nepeta L. taxa. Genet Resour Crop Evol. https://doi.org/10.1007/s10722-021-01228-y

    Article  Google Scholar 

  • Timmer JC, Enoksson M, Wildfang E, Zhu W, Igarashi Y, Denault JB, Ma Y, Dummit B, Chang YH, Mast AE, Eroshkin A, Smith JW, Tao WA, Salvesen GS (2007) Profiling constitutive proteolytic events in vivo. Biochem J 407(1):41–48. https://doi.org/10.1042/BJ20070775

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wagstaff SJ, Olmstead RG, Cantino PD (1995) Parsimony analysis of cpDNA restriction site variation in subfamily Nepetoideae (Labiatae). Am J Bot 82:886–892

    Article  CAS  Google Scholar 

  • Wu CI (2001) The genic view of the process of speciation. J Evol Biol 14:851–65

    Article  Google Scholar 

Download references

Funding

No funding to declare.

Author information

Authors and Affiliations

  1. Department of Biology, Faculty of Sciences, Arak University, Arak, 38156‒8‒8349, Iran

    Seyed Mehdi Talebi, Fatemeh Arianejad & Majid Mahdieh

  2. Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

    Masoud Sheidai

Authors
  1. Seyed Mehdi Talebi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Masoud Sheidai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fatemeh Arianejad
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Majid Mahdieh
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

All the authors contributed to the study's conception and design. SMT, MS and FA performed material preparation, data collection, and analysis. The first draft of the manuscript was written by SMT and revised by MM. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Seyed Mehdi Talebi.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

Not applicable.

Informed consent

All authors agreed to the publication of the present work.

Consent for publication

We hereby declare that we participated in this research and the paper's development. We have read its final version and give consent for the publication.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Talebi, S.M., Sheidai, M., Arianejad, F. et al. Biosystematics relationships among Marrubium L. (Lamiaceae) species in Iran. Genet Resour Crop Evol 69, 2833–2847 (2022). https://doi.org/10.1007/s10722-022-01403-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10722-022-01403-9

Keywords

Search

Navigation