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Characterization and relative mineral quantification of male gametophytes of garlic chives (Allium tuberosum Rottler ex Sprengel)

  • Notes on Neglected and Underutilized Crops
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Abstract

Garlic chives (Allium tuberosum Rottler ex Sprengel) are an underutilized and neglected food crop of the Allium genus. For the future genetic development of alliums, phenotypic characterization and insights of breeding behaviour could be the aid to breed the new garlic chives varieties. Field Emission Scanning Electron Microscopy (FESEM) was used to reveal the morphological features of male gametophytes. The anther was reticulate, conspicuous, folded surface with scaly plates, and pollen was found to be sub-ovoid morphologies with long regulate and striated sexine. Spectra from FESEM-based Energy Dispersive X-ray Spectroscopy (EDS) revealed the elemental composition and distribution. The micrograph of an anther sample revealed the presence of O, C, K, P, N, Fe, Mn, Zn, and Mg minerals, as well as C, O, N, P, K, Fe, Ca, Mn, Mg, Zn, Cu, Na, and Cl minerals in the pollen grain. These insights of male gametophytes traits and characteristics, relative mineral composition and breeding behaviour could aid in the better understanding, characterization, identification and genetic improvement of A. tuberosum.

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Data Availability

The text and Supplementary Materials provide the data that was used to accomplish the study goal. On request from the relevant author, raw data used in statistical analyses is accessible for future usage.

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Acknowledgements

Director, ICAR-Directorate of Onion and Garlic Research, Rajgurunagar, Pune for mobilizing the funds extending the research facilities.

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Authors and Affiliations

  1. ICAR-Directorate of Onion and Garlic Research, Rajgurunagar, 410505, Pune, Maharashtra, India

    Dalasanuru Chandregowda Manjunathagowda, Ashwini Prashant Benke, Vijay Mahajan & Major Singh

  2. ICAR-Directorate of Floriculture Research, Shivajinagar, 411005, Pune, Maharashtra, India

    Kiran P. Bhagat

  3. ICAR-Indian Institute of Seed Science, Mau, 275103, Uttar Pradesh, India

    Kuldip Jayaswall

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  1. Dalasanuru Chandregowda Manjunathagowda
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  2. Ashwini Prashant Benke
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  3. Kiran P. Bhagat
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  4. Vijay Mahajan
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  5. Kuldip Jayaswall
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  6. Major Singh
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Correspondence to Dalasanuru Chandregowda Manjunathagowda.

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Manjunathagowda, D.C., Benke, A.P., Bhagat, K.P. et al. Characterization and relative mineral quantification of male gametophytes of garlic chives (Allium tuberosum Rottler ex Sprengel). Genet Resour Crop Evol 69, 461–468 (2022). https://doi.org/10.1007/s10722-021-01300-7

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  • DOI: https://doi.org/10.1007/s10722-021-01300-7

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