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Allelic diversity of three anthocyanin synthesis genes in accessions of native Solanum tuberosum L. ssp. tuberosum at the Potato Genebank of the Universidad Austral de Chile

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Abstract

The potato (Solanum tuberosum L.) is the fourth most important crop for global food security. Its tubers contain high concentrations of antioxidants, especially in genotypes with red or purple pigmentation. The Potato Genebank of the Universidad Austral of Chile includes accessions that show pigmentation in their flesh and/or skin as well as high levels of anthocyanins, but little is known about the genetic basis of these characteristics. We determined the allelic diversity at the D, P and R loci which control anthocyanin biosynthesis and consequently potato tuber pigmentation in the 96 accessions with high and low levels of anthocyanins. Next-generation sequencing analysis revealed that the different allelic variants did not cluster for anthocyanin content. However, SNP584(G) in the D locus was a base mutation present in all high-anthocyanin accessions and also in 12 accessions with low levels of these metabolites. Greater allelic diversity was observed at the P locus, where alleles correlated positively with purple pigmentation in most of the accessions. The R locus showed lower allelic diversity, probably due to the lower number of red-pigmented accessions. This study provides insight into the diversity of Chilean native potatoes, enabling the molecular selection of high-pigmented accessions to assess new breeding material that can be used in applications for healthy nutrition.

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Data were generated at the Universidad Austral de Chile and Fraunhofer Institute for Molecular Biology and Applied Ecology IME.

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Acknowledgements

The authors acknowledge financial support provided by CONICYT (Grant No. BMBF20140035) in Chile and the BMBF (Grant No. 01DN15010) in Germany. The authors acknowledge Richard M. Twyman and Birgit Orthen for editing the manuscript.

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Authors and Affiliations

  1. Faculty of Agricultural Sciences, Graduate School, Universidad Austral de Chile, Valdivia, Chile

    J. L. Solís

  2. Faculty of Sciences, Institute of Biochemistry and Microbiology, Universidad Austral de Chile, Valdivia, Chile

    J. Canales

  3. Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstr. 6, 52074, Aachen, Germany

    J. Muth & D. Pruefer

  4. Institute for Plant Biology and Biotechnology, University of Muenster, Münster, Germany

    D. Pruefer

  5. Faculty of Agricultural Sciences, Institute of Plant Production and Protection, Universidad Austral de Chile, Valdivia, Chile

    C. Lizana, R. Riegel & A. Behn

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  1. J. L. Solís
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  2. J. Muth
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  4. C. Lizana
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  5. D. Pruefer
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  7. A. Behn
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Jose Luis Solis, Jost Muth, Ricardo Riegel, Carolina Lizana, Dirk Prüfer and Anita Behn. The processing of the data was done by Javier Canales. The article is based primarily on the student’s master thesis at the Universidad Austral de Chile of Jose Luis Solis and therefore he is the first author. Anita Behn is the curator of the Potato Genebank at the UACh and the Professor in charge of the Master student. The first draft of the manuscript was written by Anita Behn, Jose Luis Solis, Ricardo Riegel, Jost Muth and Dirk Prüfer and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to A. Behn.

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Solís, J.L., Muth, J., Canales, J. et al. Allelic diversity of three anthocyanin synthesis genes in accessions of native Solanum tuberosum L. ssp. tuberosum at the Potato Genebank of the Universidad Austral de Chile. Genet Resour Crop Evol 69, 297–314 (2022). https://doi.org/10.1007/s10722-021-01230-4

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