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Identification of genomic locations associated with grain micronutrients (iron and zinc) in rice (Oryza sativa L.)

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Abstract

In this investigation, recombinant inbred lines (RILs) population derived from PAU 201 (high yielding) and Palman 579 (high iron and zinc content) varieties were phenotyped in F5 and F6 generations for grain micronutrient content. The results showed high genetic variations among RILs and exceeded trait values beyond those of parents revealing transgressive segregants. Pearson’s correlation coefficient analysis showed that iron content had a significant positive correlation with zinc content in both generations. Composite interval mapping identified 16 QTLs on linkage groups 2, 6, 10, and 12. Eight QTLs were for zinc content and four for iron content, and four for grain yield per plant. The maximum number of QTLs were detected on chromosome 2, followed by chromosomes 12 and 10. The LOD score of identified QTLs varied from 3.7 (qYp10) to 18.43 (qFe2) explaining 51.8% and 52.8% phenotypic variation, respectively. Co-localization of QTLs (qZn12.2, qFe12, and qYp12) associated with zinc content, iron content, and grain yield in 12 between the marker interval RM 2734 and RM12 represents 5.1 cM distance, could be used for introgression for rice improvement through marker-assisted selection after validation.

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Abbreviations

PH:

Plant height

ET:

Effective no of tillers

PL:

Panicle length

GY:

Grain yield

TGW:

Thousand-grain weight

Fe:

Iron content (μg/g)

Zn:

Zinc content (μg/g)

RIL:

Recombinant inbred lines

QTL:

Quantitative trait loci

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Acknowledgements

This study was part of Ph. D work, conducted in Chaudhary Charan Singh Haryana Agriculture University, Hisar India. The authors are thankful to the Department of Molecular Biology, Biotechnology and Bioinformatics, CCS Haryana Agricultural University, Hisar-125004, Haryana, India for providing research facilities for conducting the research.

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Author notes

  1. Mahavir Bishnoi

    Present address: Department of Genetics and Plant Breeding, Agriculture and Forestry University, Rampur, Chitwan, Nepal

Authors and Affiliations

  1. Department of Molecular Biology, Biotechnology and Bioinformatics, CCS Haryana Agricultural University, Hisar, Haryana, 125004, India

    Amit Pippal, Rahul Kumar Meena, Mahavir Bishnoi, Pratik Istari Bhoyar & Rajinder K. Jain

  2. Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar, Haryana, 125004, India

    Nabin Bhusal

  3. Department of Genetics and Plant Breeding, Agriculture and Forestry University, Rampur, Chitwan, Nepal

    Nabin Bhusal

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  1. Amit Pippal
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Correspondence to Nabin Bhusal.

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Pippal, A., Bhusal, N., Meena, R.K. et al. Identification of genomic locations associated with grain micronutrients (iron and zinc) in rice (Oryza sativa L.). Genet Resour Crop Evol 69, 221–230 (2022). https://doi.org/10.1007/s10722-021-01222-4

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