Abstract
Brassica represents an agriculturally important and diverse group of oilseed crops with a long evolutionary history. Molecular markers played an important role in understanding origin and evolution of Brassica species. In the present research, both Single Nucleotide Polymorphisms (SNPs) and Simple Sequence Repeats (SSRs) developed from Brassica juncea L. Czern. were used to find out the phylogenetic relationship between different cultivated and wild Brassica species. A total of 88 SSR and 58 SNP markers were found to be functional across 38 genotypes belonging to ten different taxon groups. The polymorphic markers were able to group the genotypes into three different clusters and showed relatedness among different genomes based on genetic distances. The transferability of these markers serve the purpose of their quick use in cultivar identification, diversity and phylogenetic analysis in those orphan crops species where no or less genomic information is available.
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Acknowledgements
We would like to thank Dr. Manmohan Sharma of School of Biotechnology, SKUAST-Jammu for generously sharing aliquots of SSR markers.
Funding
The present work was supported by the funds received by Dr. Ravinder Singh as research grant (BT/PR3946/AGR/2/839/2011 from 2013 to 2016) from Department of Biotechnology, Government of India, New Delhi.
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RS design the experiment; JS and RS performed the experiments and written the manuscript; RM and RKS helped in the analysis of various parameters of genetic diversity.
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Sudan, J., Singh, R., Mahajan, R. et al. Deconstructing molecular phylogenetic relationship among cultivated and wild Brassica species. Genet Resour Crop Evol 68, 2281–2288 (2021). https://doi.org/10.1007/s10722-021-01198-1
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DOI: https://doi.org/10.1007/s10722-021-01198-1