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Identification of genomic regions at seedling related traits in response to aluminium toxicity using a new high-density genetic map in rice (Oryza sativa L.)

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Abstract

Aluminium toxicity is adversely affecting the rice growth and production in acidic soils of developing countries. Rice is popular and stable crop which meets food requirements of about 50% world population. A set of 122 rice backcross recombinant inbreed lines (BRILs) derived from a cross between Japonica (cv.02428) and Indica (Changhui/891) was grown in nutrient solution. High density linkage map generated by using 3075 highly stable SNP markers. Relative root length (RRL) was used as standard criteria for aluminium tolerance. A total of 23 QTLs were detected in the current study for six traits across all experimental conditions. Regarding RRL, three QTLs qCRL-6, qSRL-6, and qRRL-6 were found stable and common QTLs across all environments. Out of these three QTLs, qRRL-6 with higher heritability estimates (H2 = 96.59%) was novel QTL with a positive additive effect. In addition, shoot length (SL), shoot fresh weight (SFW), root fresh weight (RFW), shoot dry weight (SDW) and root dry weight (RDW) were used and identified QTLs, qSSL-11, qRRFW-1, and qRRDW-1 and qRRDW-8 which were newly reported QTLs. Three pleiotropic QTLs qRRFW-1, qRSDW-1, and qRRDW-1 were also reported and these QTLs would facilitate to improve Al tolerance by MAS selection and QTLs pyramiding.

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Funding

The research was supported by the National Natural Science Foundation of China (31560350, 31760350 and 7196302), the National Key Research and Development Program of China (2018YFD0301102), the Key Research and Development Program of Jiangxi Province (20171ACF60018 and 20192ACB60003), Natural Science Foundation of Jiangxi (20181BAA208055 and 20202BABL205020) and the Jiangxi Agriculture Research System (JXARS-18).

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Author notes

  1. Adnan Rasheed, Ghulam Mustafa Wassan and Hira Khanzada have contributed equally this work.

Authors and Affiliations

  1. Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education/College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, People’s Republic of China

    Adnan Rasheed, Ghulam Mustafa Wassan, Hira Khanzada, Abdul Malik Solangi, Ruicai Han, Huijie Li, Jianmin Bian & Ziming Wu

Authors
  1. Adnan Rasheed
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  2. Ghulam Mustafa Wassan
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  6. Huijie Li
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  7. Jianmin Bian
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  8. Ziming Wu
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Contributions

AR planned and conducted research work, collected data, prepared manuscript, GMW and HK designed research plan, data analysis and revised the manuscript. AMS helped in data collection, RH and HL helped in some lab work. JB provid backcross Inbred lines and ZW supervised the research.

Corresponding authors

Correspondence to Jianmin Bian or Ziming Wu.

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Rasheed, A., Wassan, G.M., Khanzada, H. et al. Identification of genomic regions at seedling related traits in response to aluminium toxicity using a new high-density genetic map in rice (Oryza sativa L.). Genet Resour Crop Evol 68, 1889–1903 (2021). https://doi.org/10.1007/s10722-020-01103-2

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