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Pollen morphology and variability of the Rosa L. species of Western Himalaya in India

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Abstract

The present study aimed to understand the pollen morphological variation among and within the Rosa L. species of Western Himalaya, India, and to investigate whether the pollen characters may help to distinguish these species of roses. The pollen morphology of nine Rosa species viz. R. canina L., R. centifolia L., R. damascena Mill., R. foetida var. persiana (Lem.) Rehder, R. moschata Herrm, R. multiflora Thunb, R. nanothamnus Boulenger, R. webbiana Wall. ex Royle, and R. macrophylla Lindl were studied. A total of 31 accessions of roses were collected from Jammu and Kashmir, Ladakh, and Himachal Pradesh. Ten quantitative characters, i.e., length of the polar axis (P), length of equatorial axis (E), exine thickness (Ex), length of colpi (LeC), width of striae (Sr), width of groove (Gr), number of striae (Ns), Polar area index (PAI) and P/E ratio were studied. The measurements and observations were carried out with both light microscopy and scanning electron microscopy. Pollen grains of all the studied species were tricolporate. Prolate and subprolate shapes were the dominant pollen shape types. The highest and lowest polar axis length was observed for R. canina (32.73 µm) and R. moschata (25.42 µm), respectively. The average colpi length in the studied species was 22.19 µm, with R. macrophylla having a minimum length of 13.42 µm and R. canina with a maximum length of 34.03 µm. Results of the principal component analysis indicated that the principal component (PC1) attributed 42.10% of the total variation, and positively associated with P, E, LeC, and Ns. Exine ornamentation almost distinguished all the species of Rosa in this study.

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Acknowledgements

Authors thank Director, IIIM Jammu, for providing necessary facilities to carry out the work. Authors are thankful to SERB DST for financial assistance under Start-Up Research Grant (Young Scientist), Grant No YSS/2015/000442. KS acknowledges the financial support provided by CSIR in the form of a JRF/SRF fellowship. CSIR-IIIM Publication No. CSIR-IIIM/IPR/00178.

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  1. Plant Science Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu, Jammu and Kashmir, 180 001, India

    Kanwaljeet Singh, P. R. Sharma & Sumeet Gairola

  2. Department of Botany, University of Jammu, Jammu, Jammu and Kashmir, India

    Kanwaljeet Singh & Yash Pal Sharma

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  1. Kanwaljeet Singh
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Singh, K., Sharma, Y.P., Sharma, P.R. et al. Pollen morphology and variability of the Rosa L. species of Western Himalaya in India. Genet Resour Crop Evol 67, 2129–2148 (2020). https://doi.org/10.1007/s10722-020-00967-8

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