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Characterization of genetic variation and antioxidant properties in strawberry (Fragaria × ananassa Duch.) mutant genotypes

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Abstract

Strawberry (Fragaria × ananassa Duch.) is an important fruit in the food industry. Recently, several strawberry mutant genotypes with improved agronomic traits have been developed using gamma irradiation and somaclonal variation mutagenesis. This study was conducted to examine the genetic diversity and variation among 19 novel strawberry genotypes and 3 commercial strawberry cultivars using single nucleotide polymorphism (SNP) markers generated from genotyping-by-sequencing (GBS) analysis. In addition, the bio-functional compounds of the various strawberry genotypes were examined to determine the fruit’s potential for additional uses in the food industry. Using GBS sequencing data, a total of 506,916,648 clean data reads were generated, with 376,723,429 (74.3%) mapped reads. A set of 55,733 filtered SNPs was used to perform a phylogenetic analysis, which showed that there were clear differences among the strawberry genotypes based on their original cultivar, except for two mutant genotypes. The ellagic acid levels of 22 strawberry genotypes ranged from 1.71 to 12.10 mg/100 g with an average of 4.36 mg/100 g. Pelargonidin-3-glucoside was the major anthocyanin in 17 strawberry genotypes; however, cyanidin-3-glucoside was the major anthocyanin in two purple flower genotypes. Anthocyanin pigments were not detectable in the three white fruit genotypes. This study demonstrated that the SNPs and functional compound content are an efficient tool for mutant screening and for the selection of elite genotypes in strawberry breeding programs.

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Abbreviations

NGS:

Next-generation sequencing

SNPs:

Single nucleotide polymorphisms

GBS:

Genotyping-by-sequencing

GO:

Gene ontology

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Acknowledgements

This work was supported by Grants from the National Research Foundation of Korea (NRF) (NRF-2018M2A2A6A05057264), Republic of Korea.

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Author notes

  1. Jaihyunk Ryu and Woon Ji Kim have contributed equally to this work.

Authors and Affiliations

  1. Applied Plant Science, Chonnam National University, Gwangju, 61186, Korea

    Woon Ji Kim, Sang Hun Kim & Bo-Keun Ha

  2. Bioplus Co., Wanju, 55310, Korea

    Kang-Seop Lee, Han-Jig Jo & Ee-Youb Kim

  3. Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongup, 56212, Korea

    Jaihyunk Ryu, Sang Hoon Kim & Si-Yong Kang

  4. Seeders Inc., Daejeon, 34015, Korea

    Jeong-Hee Lee

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  1. Jaihyunk Ryu
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Correspondence to Bo-Keun Ha.

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Ryu, J., Kim, W.J., Kim, S.H. et al. Characterization of genetic variation and antioxidant properties in strawberry (Fragaria × ananassa Duch.) mutant genotypes. Genet Resour Crop Evol 67, 1457–1471 (2020). https://doi.org/10.1007/s10722-020-00918-3

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