Skip to main content
SpringerLink
Log in

Genetic inheritance and identification of germplasm sources for anthracnose resistance in soybean [Glycine max (L.) Merr.]

  • Research Article
  • Published:
Genetic Resources and Crop Evolution Aims and scope Submit manuscript

Abstract

Soybean anthracnose caused by Colletotrichum truncatum (Schw.) Andrus & W.D. Moore is a devastating disease in Central and Southern India. Chemical control of this disease is neither economical nor eco-friendly and genetic resistance is considered to be an efficient means for its management. Therefore, in the current study, a total of 225 soybean [Glycine max (L.) Merr.] germplasm accessions were evaluated for anthracnose resistance which resulted in the identification of five genotypes viz., EC 538828, EC 34372, EC 457254, AKSS 67 and Karune as highly resistant. Genetics of anthracnose resistance in three F2 populations derived from EC 34372 × JS 95-60, EC 457254 × JS 95-60 and AKSS 67 × JS 95-60 revealed that the resistance in all the three resistant parents was governed by two major genes interacting in complementary fashion. The χ2 test for goodness of fit revealed that F2 plants in each of the three populations segregated in 9 resistant: 7 susceptible ratio. This is the first report on genetics of anthracnose resistance in soybean and will aid soybean breeders to develop a strategic anthracnose resistance breeding program and to map the genes governing resistance. Research findings from this study indicated the potential role of exotic germplasm in Indian soybean improvement against anthracnose disease.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Baker EP (1966) Isolation of complementary genes conditioning crown rust resistance in oat variety Bond. Euphytica 15:313–318

    Article  Google Scholar 

  • Basandrai D, Basandrai AK, Sharma V, Devlash R, Gautam NK (2011) Evaluation for multiple resistances to yellow mosaic, anthracnose, Cercospora leaf spot and powdery mildew in urdbean germplasm. Plant Dis Res 26(2):166–167

    Google Scholar 

  • Bhadauria V, Bett KE, Zhou T, Vandenberg A, Wei Y, Banniza S (2013) Identification of lens culinaris defense genes responsive to the anthracnose pathogen Colletotrichum truncatum. BMC Genet 14:31

    Article  CAS  Google Scholar 

  • Bhattacharyya PK, Ram HH, Kole PC (1999) Inheritance of resistance to yellow mosaic virus in inter-specific crosses of soybean. Euphytica 108:157–159

    Article  Google Scholar 

  • Bhor TJ, Chimote VP, Deshmukh MP (2015) Molecular tagging of Asiatic soybean rust resistance in exotic genotype EC 241780 reveals complementation of two genes. Plant Breed. https://doi.org/10.1111/pbr.12240

    Article  Google Scholar 

  • Chen LS, Chu C, Liu CD, Chen RS, Tsay JG (2006) PCR based detection and differentiation of anthracnose pathogens, Colletotrichum gloeosporioides and C. truncatum, from vegetable soybean in Taiwan. J Phytopathol 154:654–662

    Article  CAS  Google Scholar 

  • CIAT (Centro Internacional de Agricultura Tropical) (1987) Standard system for the evaluation of bean germplasm. van Schoonhoven, A., and Pastor-Corrales, M.A. (compilers). The International Center for Tropical Agriculture, Cali, Colombia

  • Damm U, Woudenberg JHC, Cannon PF, Crous PW, Damm U (2009) Colletotrichum species with curved conidia from herbaceous hosts. Fungal Divers 39:45–87

    Google Scholar 

  • Dracatos PM, Zhang P, Park RF, McIntosh RA, Wellings CR (2016) Complementary resistance genes in wheat selection ‘Avocet R’ confer resistance to stripe rust. Theor Appl Genet 129:65. https://doi.org/10.1007/s00122-015-2609-7

    Article  CAS  PubMed  Google Scholar 

  • Gossen BD, Anderson KL, Buchwaldt L (2009) Host specificity of Colletotrichum truncatumfrom lentil. Can J Plant Pathol 31:65–73

    Article  Google Scholar 

  • Hartman GL, Manandhar JB, Sinclair JB (1986) Incidence of Colletotrichum spp. on soybeans and weeds in Illinois and pathogenicity of Colletotrichum truncatum. Plant Dis 70:780–782

    Article  Google Scholar 

  • ICAR (2009) Handbook of agriculture. Indian Council of Agricultural Research, New Delhi, pp 1143–1150

    Google Scholar 

  • Jagtap GP, Sontakke PL (2009) Taxonomy and morphology of Colletotrichum truncatum isolates pathogenic to Soybean. Afr J Agric Res 4(12):1483–1487

    Google Scholar 

  • Kumawat G, Singh G, Gireesh C, Shivakumar M, Arya M, Agarwal DK, Husain SM (2015) Molecular characterization and genetic diversity analysis of soybean (Glycine max (L.) Merr.) germplasm accessions in India. Physiol Mol Biol Plants 21:101–107. https://doi.org/10.1007/s12298-014-0266-y

    Article  CAS  PubMed  Google Scholar 

  • Kumawat G, Shivakumar M, Rajesh V, Chandra S, Kamble V, Nataraj V, Tripathi R, Agrawal N, Satpute GK, Ratnaparkhe MB, Gupta S, Bhatia VS (2018) Allelic characterization of five flowering genes in soybean germplasm using gene specific markers. In: abstract book of 4th International Plant Physiology Congress-2018, at CSIR-NBRI, Lucknow, 02–05 December, 2018. pp 249

  • Ma G, Chen P, Buss GR, Tolin SA (2002) Complementary action of two independent dominant genes in Columbia soybean for resistance to soybean mosaic virus. J Hered 93(3):179–184

    Article  CAS  Google Scholar 

  • Manandhar JB, Hartman GL, Sinclair JB (1988) Soybean germplasm evaluation for resistance to Colletotrichum truncatum. Plant Dis 72:56–59

    Article  Google Scholar 

  • Manandhar HK, Timila RD, Sharma S, Joshi S, Manandhar S, Gurung SB, Sthapit S, Palikhey E, Pandey A, Joshi BK, Manandhar G, Gauchan D, Jarvis DI, Sthapit BR (2016) A field guide for identification and scoring methods of diseases in the mountain crops of Nepal. NARC, DoA, LI-BIRD and Bioversity International, Nepal, p 77

    Google Scholar 

  • Nagaraj BT, Jahagirdar S, Basavaraja GT (2014) Identification of resistant sources in glass house and field evaluation of soybean genotypes to anthracnose caused by Colletotrichum truncatum (Schw.) Andrus and Moore. The Bioscan 9(3):1333–1336

    Google Scholar 

  • O’Connel RJ, Uronu AB, Waksman G, Nash C, Keon JPR, Bailey JA (1993) Hemibiotrophic infection of Pisum sativum by Colletotrichum truncatum. Plant Pathol 42(5):774–778

    Article  Google Scholar 

  • Rahangdale SR, Raut VM (2004) Genetics of rust resistance in soybean [Glycine max (L.) Merrill]. Indian J Genet Plant Breed 64(2):121–124

    Google Scholar 

  • Rani A, Kumar V, Gill BS, Rathi P, Shukla S, Singh RK, Husain SM (2017) Linkage mapping of Mungbean yellow mosaic India virus (MYMIV) resistance gene in soybean. Breed Sci 67:95–100

    Article  CAS  Google Scholar 

  • Rani A, Kumar V, Gill BS, Shukla S, Rathi P, Singh RK (2018) Mapping of duplicate dominant genes for Mungbean yellow mosaic India virus resistance in Glycine soja. Crop Sci 58:1–9. https://doi.org/10.2135/cropsci2017.11.0652

    Article  CAS  Google Scholar 

  • Rao S, Nandineni MR (2017) Genome sequencing and comparative genomics reveal a repertoire of putative pathogenicity genes in chilli anthracnose fungus Colletotrichum truncatum. PLoS ONE 12(8):e0183567. https://doi.org/10.1371/journal.pone.0183567

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sharma AN, Gupta GK, Verma RK, Sharma OP, Bhagat S, Amaresan N, Saini MR, Chattopadhyay C, Sushil SN, Asre R, Kapoor KS, Satyagopal K and Jeyakumar P (2014) Integrated pest management for soybean. p 3

  • Sinclair JB (1982) Compendium of soybean diseases, 2nd edn. American Phytopathological Society, St. Paul, p 104

    Google Scholar 

  • Sinclair JB, Backman PA (1989) Compendium of soybean diseases, 3rd edn. American Phytopathological Society, St. Paul

    Google Scholar 

  • Singh RP, McIntosh RA (1984) Complementary genes for reaction to Puccinia recondita I. Genetic and linkage studies. Can J Genet Cytol 26:723–735

    Article  Google Scholar 

  • Singh D, Park R, McIntosh RA (1999) Genetic relationship between the adult plant resistance gene Lr12 and the complementary gene Lr31 for seedling resistance to leaf rust in common wheat. Plant Pathol 48:567–573

    Article  CAS  Google Scholar 

  • Soybean News (2018). ICAR- Indian Institute of Soybean Research, Indore (Issue 25). https://iisrindore.icar.gov.in/

  • Talukdar A, Harish GD, Shivakumar M, Kumar B, Verma K, Lal SK, Sapra RL, Singh KP (2013) Genetics of yellow mosaic virus (YMV) resistance in cultivated soybean [Glycine max (L.) MERR.]. Legume Res 36(3):263–267

    Google Scholar 

  • Wrather A, Shanon G, Balardin R, Carregal L, Escobar R, Gupta GK, Ma Z, Morel W, Ploper D, Tenuta A (2010) Effect of diseases on soybean yield in the top 8 producing countries in 2006. Plant Health Progr. https://doi.org/10.1094/PHP-2010-0102-01-RS

    Article  Google Scholar 

  • Wu J, Ding J, Du Y, Xu Y, Zhang X (2007) Genetic analysis and molecular mapping of two dominant complementary genes determining resistance to sugarcane mosaic virus in maize. Euphytica 156:355–364

    Article  CAS  Google Scholar 

  • Yang HC, Hartman GL (2015) Methods and evaluation of soybean genotypes for resistance to Colletotrichum truncatum. Plant Dis 99:143–148

    Article  Google Scholar 

Download references

Acknowledgements

Authors gratefully acknowledge Indian Institute of Soybean Research for supporting this investigation. Authors thank Devendra P. Yadav for technical assistance during the investigation.

Author information

Author notes

  1. Vennampally Nataraj and Shivakumar Maranna have equally contributed to this work.

Authors and Affiliations

  1. ICAR-Indian Institute of Soybean Research, Indore, India

    Vennampally Nataraj, Shivakumar Maranna, Giriraj Kumawat, Sanjay Gupta, Laxman Singh Rajput, Sanjeev Kumar, Amar Nath Sharma & Virender Singh Bhatia

Authors
  1. Vennampally Nataraj
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shivakumar Maranna
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Giriraj Kumawat
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sanjay Gupta
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Laxman Singh Rajput
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Sanjeev Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Amar Nath Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Virender Singh Bhatia
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Conceived and designed the experiments: SM, NV; Performed the experiments: SM, NV, GK, SK, LSR; Analyzed the data: NV; Contributed to the writing of the manuscript: NV, SM, GK; Development of F1 populations: SM, GK; Screening of germplasm and F2 Population: NV, SM, SG; Contributed through intellectual input: VSB, ANS; All the authors read and approved the manuscript.

Corresponding author

Correspondence to Shivakumar Maranna.

Ethics declarations

Conflict of interest

Authors declare that they have no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (XLSX 20 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Nataraj, V., Maranna, S., Kumawat, G. et al. Genetic inheritance and identification of germplasm sources for anthracnose resistance in soybean [Glycine max (L.) Merr.]. Genet Resour Crop Evol 67, 1449–1456 (2020). https://doi.org/10.1007/s10722-020-00917-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10722-020-00917-4

Keywords

Search

Navigation