Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Realizing hybrids between the cultivated peanut (Arachis hypogaea L.) and its distantly related wild species using in situ embryo rescue technique

Abstract

Cross-incompatibility between peanut cultigen and species outside section Arachis, impedes the transfer of desirable traits such as high resistance, good quality and high productivity from incompatible species in cultivar development. To recover hybrids from 4 incompatible crosses involving 3 wild species, viz., A. pusilla Benth., A. appresipilla Krapov. & W.C. Greg. and A. paraguariensis Chodat & Hassl., an in situ embryo rescue technique was exploited, resulting in hybrid seeds from all the 4 crosses. The key of this technique is to apply growth substances to pollinated flower bases, and the next step is to wait and harvest seeds directly, whose hybridity is to be tested by miniature inverted-repeat transposable element markers. The technique is advantageous over its in vitro counterpart in earlier intervention, easier operation, and shorter duration required for obtaining hybrids. These superiorities warrant its wide use in the genus Arachis as well as in other plant taxa where post-zygotic hybridization barriers also exist.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2
Fig. 3

References

  1. Mallikarjuna N (2003) Wide hybridization in important food legumes. In: Jaiwal PK, Singh RP (eds) Improvement strategies of leguminosae biotechnology. Kluwer Academic Publishers, Dordrecht, pp 151–171

  2. Mallikarjuna N, Shilpa K, Pandey M, Janila P, Varshney R (2014) Groundnut. In: Singh M, Bisht IS, Dutta M (eds) Broadening the genetic base of grain legumes. Springer, Heidelberg, pp 161–172

  3. Shirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Akiko Watanabe A, Fujishiro T, Isobe S (2012) Characterization of active miniature inverted-repeat transposable elements in the peanut genome. Theoret Appl Genet 124:1429–1438

  4. Shivanna KR, Bahadur B (2015) Efficacy of biotechnological approaches to raise wide sexual hybrids. In: Bahadur B, Rajam MV, Sahijram L, Krishnamurthy KV (eds) Plant biology and biotechnology: Volume II: Plant genomics and biotechnology, vol II. Springer, India, pp 347–362

  5. Varshney RK, Bertioli DJ, Moretzsohn MC, Vadez V, Krishnamurthy L, Aruna R, Nigam SN, Moss BJ, Seetha K, Ravi K, Knapp HSJ, Hoisington DA (2009) The first SSR-based genetic linkage map for cultivated groundnut (Arachis hypogaea L.). Theoret Appl Genet 118:729–739

  6. Wang CT, Shen FY, Qiu QS, Xu JZ. (1999) In vitro culture of peanut (Arachis sp.) peg. Trop Agric Res Ext. 2(1): 17–20

  7. Wang CT, Yu HT, Wang XZ, Tang YY, Wu Q, Gao HY, Hu DQ, Song GS, Chen JH, Yu SL (2012) Production of peanut hybrid seeds in an intersectional cross through post-pollination treatment of flower bases with plant growth regulators. Plant Growth Regul 68:511–515

  8. Wang CT, Zhang JC, Tang YY, Guan SY, Wang XZ, Wu Q, Shan L, Zhu LG, Su JW, Yu ST (eds) (2013) Peanut genetic improvement. Shanghai Science and Technology Press, Shanghai

  9. Wang CT, Tang YY, Wang XZ, Wu Q, Yang Z, Song GS, Gao HY, Yu ST, Ni WL, Yang TR, Li M, Qian L (2014) Utilizing wild incompatible Arachis species in the genetic improvement of the cultivated peanut (A. hypogaea L.). In: Kumar PA (ed) Biotechnology, vol 2, Plant biotechnology. Studium Press LLC, Houston, pp 91–114

  10. Yu ST, Wang CT, Yu SL, Wang XZ, Tang YY, Chen DX, Zhang JC (2010) Simple method to prepare DNA templates from a slice of peanut cotyledonary tissue Polymerase Chain Reaction. Electron J Biotechnol 13(4). https://scielo.conicyt.cl/scielo.php?pid=S0717-34582010000400012&script=sci_arttext&tlng=en

  11. Yu SL, Wang CT, Yang QL, Zhang DX, Zhang XY, Cao YL, Liang XQ, Liao BS (eds) (2011) Peanut genetics and breeding in China. Shanghai Science and Technology Press, Shanghai

  12. Zhang XY, Xu J, Tang FS, Dong WZ, Zang XW, Zhang ZX (2013). Embryonic development and changes of endogenous hormones in interspecific hybrids between peanut (Arachis hypogaea L.) and wild Arachis Species. Acta Agronomica Sinca. 39(6): 1127–1133

Download references

Funding

This study was funded by Ministry of Agriculture, People’s Republic of China (CARS-13), Xinjiang Production and Construction Corps, People’s Republic of China (2018BC012), Yantai Science and Technology Bureau, People’s Republic of China (2018ZDCX), and Taishan Industry Leading Talents Special Fund (LJNY201808).

Author information

Correspondence to Chuan Tang Wang.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Wang, C.T., Wang, X.Z., Wang, Z.W. et al. Realizing hybrids between the cultivated peanut (Arachis hypogaea L.) and its distantly related wild species using in situ embryo rescue technique. Genet Resour Crop Evol 67, 1–8 (2020). https://doi.org/10.1007/s10722-019-00862-x

Download citation

Keywords

  • Peanut
  • Wild species
  • Cross-incompatibility
  • Hybridization barrier
  • In situ embryo rescue