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Genetic Resources and Crop Evolution

, Volume 66, Issue 1, pp 165–175 | Cite as

Germplasm collection, genetic diversity and on-farm conservation of cowpea [Vigna unguiculata (L.) Walp.] landraces from Apulia region (southern Italy)

  • Lucia LioiEmail author
  • Anita Morgese
  • Salvatore Cifarelli
  • Gabriella Sonnante
Research Article
  • 219 Downloads

Abstract

Cowpea [Vigna unguiculata (L.) Walp.] is a major tropical pulse in a number of countries and especially in sub-Saharan Africa where this species was domesticated. Although as niche product, a remarkable number of cowpea landraces are cultivated in the Mediterranean basin, primarily for human consumption of the grains and fresh pods. In this study, within the frame of a regional project devoted to the safeguard and management of local horticultural genetic resources, some germplasm explorations were carried out in Apulia region (southern Italy) to collect, among other crops, cowpea landraces still present in the area and promote on-farm conservation of this traditional material. A second objective was to investigate the efficiency of SSR markers in detecting genetic polymorphism in cowpea landraces from a restricted geographical area. In total, 13 samples of V. unguiculata were gathered, 12 of cultivar group unguiculata and 1 of cultivar group sesquipedalis. Genetic diversity and phylogenetic relationships among 13 Apulian accessions, 9 samples from various provenances and 1 var. spontanea accession were evaluated using SSR molecular markers. A set of 19 SSR primer pairs successfully identified closely related accessions and were able to separate the different cultivar groups of V. unguiculata.

Keywords

Cowpea Genetic diversity Landraces Molecular markers On-farm conservation SSR Vigna unguiculata 

Notes

Acknowledgements

We thank Francesco P. Losavio and Giuseppe Sonnante for technical help in the material and data collection.

Funding

This research was funded by the project BiodiverSO – PSR Puglia 2007-2013 Mis. 214/4 subaz. a) and by the project BiodiverSO – PSR Puglia 2014-2020 Mis.10.2.

Supplementary material

10722_2018_703_MOESM1_ESM.pdf (5.5 mb)
Pictures showing morphological characters of seeds from Vigna unguiculata accessions used in this study. (For number correspondence see Table 1) (PDF 5630 kb)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Institute of Biosciences and Bioresources (IBBR), National Research Council (CNR)BariItaly

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