Advertisement

Genetic Resources and Crop Evolution

, Volume 65, Issue 5, pp 1455–1469 | Cite as

Characterization of a world collection of Agropyron cristatum accessions

  • Alejandro Copete
  • Roberto Moreno
  • Adoración Cabrera
Research Article
  • 110 Downloads

Abstract

The genetic diversity was studied of 115 Agropyron cristatum accessions from 17 countries. Tetraploids were the most common (74.8%), followed by diploid (16.3%) and hexaploid (6.9%). We observed a relation between geographic distribution and ploidy level. The tetraploids, the most widespread, were found from Europe through Russia to East Asia. The diploids appeared over the same general range, except in Turkey, Iran and Georgia where no diploid accessions were found. Hexaploid accessions mainly came from a region comprising the east of Turkey, the north of Iran and Georgia. A selection of 71 accessions, including all three ploidy levels, were analyzed by capillary electrophoresis using six wheat simple sequence repeat (SSR) markers. All markers presented high levels of polymorphism, generating 166 different alleles ranging in size between 84 and 256 bp. Based on polymorphic information content values obtained (0.579–0.968), all the SSRs were classified as informative markers (values > 0.5). According to the dendrogram generated, all the A. cristatum accessions were distinctly classified. Diploid, tetraploid and hexaploid accessions are not clearly differentiated from each other on the basis of SSR markers. A field experiment was conducted to morphologically characterize 18 accessions including the three ploidy levels. Significant differences were found between the accessions in spike length, spike width and number of spikelets per spike. All the cytological, molecular, and morphological data demonstrate the high genetic diversity present in A. cristatum, making it a valuable resource for future breeding programs.

Keywords

Agropyron cristatum Crested wheatgrass Ploidy level Flow cytometry Genetic diversity SSR 

Notes

Acknowledgements

This research was funded by Grant AGL2014-52445-R from the Ministerio de Economía y Competitividad, co-financed by the European Regional Development Fund. The authors declare that they have no conflicts of interest.

References

  1. Asay KH (1992) Breeding potentials in perennial Triticeae grasses. Hereditas 116:167–173CrossRefGoogle Scholar
  2. Asay KH, Dewey DR (1979) Bridging ploidy differences in crested wheatgrass with hexaploid x diploid hybrids. Crop Sci 19:519–523CrossRefGoogle Scholar
  3. Asay KH, Jensen KB (1996) Wheatgrasses. In: Moser LE, Buxton DR, Casler MD (eds) Cool-season forage grasses. Agronomy Monograph no. 34, Chap. 22. ASA-CSSA-SSSA, Madison, WI, USA, pp 691–724Google Scholar
  4. Asay KH, Jensen KB, Johnson DA, Chatterton NJ, Hansen WT, Horton WH, Young SA (1995) Registration of ‘Douglas’ crested wheatgrass. Crop Sci 35:1510–1511Google Scholar
  5. Asay KH, Chatterton NJ, Jensen KB, Jones TA, Waldron BL, Horton WH (2003) Breeding improved grasses for semiarid rangelands. Arid Land Res Manag 17:469–478CrossRefGoogle Scholar
  6. Botstein G, White RL, Skolnick M, Davis RW (1980) Construction of genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331PubMedPubMedCentralGoogle Scholar
  7. Che YH, Li HJ, Yang YP, Yang XM, Li XQ, Li LH (2008) On the use of SSR markers for the genetic characterization of the Agropyron cristatum (L.) Gaertn. in northern China. Genet Resour Crop Evol 55:389–396CrossRefGoogle Scholar
  8. Che YH, Yang YP, Yang XM, Li XQ, Li LH (2011) Genetic diversity between ex situ and in situ samples of Agropyron cristatum (L.) Gaertn. based on simple sequence repeat molecular markers. Crop Pasture Sci 62:639–644CrossRefGoogle Scholar
  9. Chen Q, Jahier J, Cauderon Y (1989) Production and cytogenetic analysis of BC1, BC2, and BC3 progenies of an intergeneric hybrid between Triticum aestivum (L.) Thell. and tetraploid Agropyron cristatum (L.) Gaertn. Theor Appl Genet 84:698–703Google Scholar
  10. Chen SY, Ma X, Zhang XQ, Huang LK, Zhou JN (2013) Genetic diversity and relationships among accessions of five crested wheatgrass species (Poaceae: Agropyron) based on gliadin analysis. Genet Mol Res 12:5704–5713CrossRefPubMedGoogle Scholar
  11. Copete A, Cabrera A (2017) Chromosomal location of genes for resistance to powdery mildew in Agropyron cristatum and mapping of conserved orthologous set molecular markers. Euphytica 213:189–297CrossRefGoogle Scholar
  12. Dewey DR (1969) Hybrids between tetraploid and hexaploid crested wheatgrass. Crop Sci 9:787–791CrossRefGoogle Scholar
  13. Dewey DR (1973) Hybrids between diploid and hexaploid crested wheatgrass. Crop Sci 13:474–477CrossRefGoogle Scholar
  14. Dewey DR (1984) The genomic system of classification as a guide to intergeneric hybridization with the perennial Triticeae. In: Gustafson JP (ed) Gene manipulation in plant improvement, 16th Stadler Genetics Symposium. Plenum Press, New York, pp 209–279CrossRefGoogle Scholar
  15. Dewey DR, Asay KH (1982) Cytogenetic and taxonomic relationships among three diploid crested wheatgrasses. Crop Sci 22:645–650CrossRefGoogle Scholar
  16. Döležel J, Greilhuber J, Suda J (2007) Estimation of nuclear DNA content in plants using flow cytometry. Nat Protoc 2:2233–2244CrossRefPubMedGoogle Scholar
  17. Dong Y, Zhou R, Xu S, Li L, Cauderon Y, Wang R (1992) Desirable characteristics in perennial Triticeae collected in China for wheat improvement. Hereditas 116:175–178CrossRefGoogle Scholar
  18. García P, Monte JV, Casanova C, Soler C (2002) Genetic similarities among Spanish populations of Agropyron, Elymus and Thinopyrum, using PCR-based markers. Genet Resour Crop Evol 49:103–109CrossRefGoogle Scholar
  19. Gul ZD, Yolcu H, Tan M, Serin Y, Gul I (2013) Yield, quality, and other characteristics of selected lines of crested wheatgrass. J Plant Regist 7:373–377CrossRefGoogle Scholar
  20. Guo Q, Meng L, Mao P, Tian X (2014) An assessment of Agropyron cristatum tolerance to cadmium contaminated soil. Biol Plant 58:174–178CrossRefGoogle Scholar
  21. Han H, Bai L, Su J, Zhang J, Song L, Gao A, Yang X, Li X, Liu W, Li L (2014) Genetic rearrangements of six wheat-Agropyron cristatum 6P addition lines revealed by molecular markers. PLoS ONE 9:e91066CrossRefPubMedPubMedCentralGoogle Scholar
  22. Hanson WD (1959) Minimum family sizes for the planning of genetic experiments. Agron J 51:711–715CrossRefGoogle Scholar
  23. Hsiao C, Asay KH, Dewey DR (1989) Cytogenetic analysis of interspecific hybrids and amphiploids between two diploid crested wheatgrasses Agropyron mongolicum and A. cristatum. Genome 32:1079–1084CrossRefGoogle Scholar
  24. Jensen KB, Larson SR, Waldron BL, Asay KH (2005) Cytogenetic and molecular characterization of hybrids between 6x, 4x, and 2x ploidy levels in crested wheatgrass. Crop Sci 46:105–112CrossRefGoogle Scholar
  25. Knowles RP (1955) A study of variability in crested wheatgrass. Can J Bot 33:534–546CrossRefGoogle Scholar
  26. Limin AE, Fowler DB (1990) An interspecific hybrid and amphiploid produced from Triticum aestivum crosses with Agropyron cristatum and Agropyron desertorum. Genome 33:581–584CrossRefGoogle Scholar
  27. Martín A, Cabrera A, Esteban E, Hernández P, Ramirez M, Rubiales D (1999) A fertile amphiploid between diploid wheat (Triticum tauschii) and crested wheatgrass (Agropyron cristatum). Genome 42:519–524CrossRefPubMedGoogle Scholar
  28. Mattera G, Avila MC, Atienza SG, Cabrera A (2015) Cytological and molecular characterization of wheat-Hordeum chilense chromosome 7Hch introgression lines. Euphytica 203:165–176CrossRefGoogle Scholar
  29. Mellish A, Coulman B (2002) Morphological characteristics of crested wheatgrass populations of diverse origin. Can J Plan Sci 82:693–699CrossRefGoogle Scholar
  30. Mellish A, Coulman B, Ferdinandez Y (2002) Genetic relationships among selected crested wheatgrass cultivars and species determined on the basis of AFLP markers. Crop Sci 42:1662–1668CrossRefGoogle Scholar
  31. Meng L, Guo Q, Mao P, Tian X (2013) Accumulation and tolerance characteristics of zinc in Agropyron cristatum plants exposed to zinc-contaminated soil. Bull Environ Contam Toxicol 91:298–301CrossRefPubMedGoogle Scholar
  32. Miller EK, Dyer WE (2002) Phytoremediation of pentachlorophenol in the crested wheatgrass (Agropyron cristatum x desertorum) rhizosphere. Int J Phytoremediation 4:223–238CrossRefGoogle Scholar
  33. Murray M, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4326CrossRefPubMedPubMedCentralGoogle Scholar
  34. Ochoa V, Madrid E, Said M, Rubiales D, Cabrera A (2015) Molecular and cytogenetic characterization of a common wheat-Agropyron cristatum chromosome translocation conferring resistance to leaf rust. Euphytica 201:89–95CrossRefGoogle Scholar
  35. Ray IM, Ab Frank, Berdahl JD (1997) Genetic variances of agronomic and morphological traits of diploid crested wheatgrass. Crop Sci 37:1503–1507CrossRefGoogle Scholar
  36. Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023PubMedPubMedCentralGoogle Scholar
  37. Sadasivaiah RS, Weijer J (1981) The origin and meiotic behaviour of hexaploid northern wheatgrass (Agropyron dasystachym). Chromosoma 82:121–132CrossRefGoogle Scholar
  38. Said M, Cabrera A (2009) A physical map of chromosome 4Hch from Hordeum chilense containing SSR, STS and EST-SSR molecular markers. Euphytica 167:253–259CrossRefGoogle Scholar
  39. Said M, Recio R, Cabrera A (2012) Development and characterisation of structural changes in chromosome 3Hch from Hordeum chilense in common wheat and their use in physical mapping. Euphytica 188:429–440CrossRefGoogle Scholar
  40. Soliman MH, Rubiales D, Cabrera A (2001) A fertile amphiploid between durum wheat (Triticum turgidum) and the × Agroticum Amphiploid (Agropyron cristatum × T. tauschii). Hereditas 135:183–186CrossRefPubMedGoogle Scholar
  41. Soliman MH, Cabrera A, Sillero JC, Rubiales D (2007) Genomic constitution and expression of disease resistance in Agropyron cristatum × durum wheat derivatives. Breed Sci 57:17–21CrossRefGoogle Scholar
  42. Tai W, Dewey DR (1966) Morphology, cytology and fertility of diploid and colchicine-induced tetraploid crested wheatgrass. Crop Sci 6:223–226CrossRefGoogle Scholar
  43. Vogel KP, Arumuganathan K, Jensen KB (1999) Nuclear DNA content of perennial grasses of the Triticeae. Crop Sci 39:661–667CrossRefGoogle Scholar
  44. Wang RR (2011) Agropyron and Psathyrostachys. In: Kole Chittaranjan (ed) Wild crop relatives: genomic and breeding resources, cereals, Chapter 2. Springer, Berlin and Heidelberg, pp 77–108CrossRefGoogle Scholar
  45. Yang CT, Fan X, Wang XL, Gu MX, Wang Y, Sha LN, Zhang HQ, Kang HY, Xiao X, Zhou YH (2014) Karyotype analysis of Agropyron cristatum (L.) Gaertner. Caryologia 67:234–237CrossRefGoogle Scholar
  46. Yousofi M, Aryavand A (2004) Determination of ploidy levels of some populations of Agropyron cristatum (Poaceae) in Iran by flow cytometry. Iran J Sci Technol Trans A Sci 28:137–144Google Scholar
  47. Yousofi M, Esmaeili M, Otroshy M (2013) Genetic variation among natural populations of Agropyron cristatum (Poaceae) based on SDS-PAGE of seed proteins. Iran J Bot 19:186–193Google Scholar
  48. Zhang J, Zhang J, Liu W, Han H, Lu Y, Yang X, Li L (2015) Introgression of Agropyron cristatum 6P chromosome segment into common wheat for enhanced thousand-grain weight and spike length. Theor Appl Genet 128:1827–1837CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of GeneticsETSIAM, University of Córdoba, Campus de Excelencia Internacional Agroalimentario, CeiA3CórdobaSpain

Personalised recommendations