Molecular and phenotypic diversity of ICARDA spring barley (Hordeum vulgare L.) collection

Abstract

Plant breeders are interested in using diverse genotypes in hybridization that can segregate for traits of importance with possibility of selection and genetic gain. Information on molecular and agro-morphological diversity helps the breeders reduce the effort for parental selection and helps the advancement of generations. A phenotypic and molecular diversity study, using 24 traits (agronomic and disease) and 6519 SNPs in a diverse collection of 336 spring barley genotypes, was carried out at Marchouch and Jemma Shiam research stations in Morocco. Based on structure and multivariate analyses, strong differentiation between the two- and six-row types were observed. The linkage disequilibrium (LD) decay of the current collection (for the combined population) was up to 3.58 cM (r 2 = 0.15) while LD decay were estimated 3.91 and 2.36 cM for two- and six-row barley, respectively. PCA of agro-morphological traits revealed grain per spike, net form of net blotch (NFNB), spot form of net blotch (SFNB), and 1000 kernel weight were the most discriminatory traits in the current collection. Association mapping in the two independent populations will be ideal for identification of markers, and QTL related to traits. The generated information on relatedness between individuals will help identify diverse genotypes for breeding programs.

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Acknowledgements

This manuscript is an output of research projects funded by CGIAR Research Program on Dryland Cereals (CRP-DC) and USAID-Linkage Program in ICARDA. The financial support to first author in the form of the CRP-DC scholarship is highly acknowledged. There is no financial or intellectual conflict of interest among the researchers involved in the current study. The authors extend thanks to Dr. Carrie Selin, Department of Plant Sciences, University of Manitoba, for editing and improving English of this manuscript. The authors are highly thankful to Dr. Carrie Selin, Dept. of Plant Sciences, U of Manitoba, for English editing.

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Correspondence to Sanjaya Gyawali.

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Amezrou, R., Gyawali, S., Belqadi, L. et al. Molecular and phenotypic diversity of ICARDA spring barley (Hordeum vulgare L.) collection. Genet Resour Crop Evol 65, 255–269 (2018). https://doi.org/10.1007/s10722-017-0527-z

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Keywords

  • Barley
  • Diversity
  • Genetic
  • Hordeum vulgare
  • Phenotypic
  • SNP
  • Structure