Genetic Resources and Crop Evolution

, Volume 63, Issue 3, pp 401–407

Development and validation of chloroplast DNA markers to assist Aegilops geniculata and Aegilops neglecta germplasm management

  • Patricia Giraldo
  • Magdalena Ruiz
  • Marta Rodríguez-Quijano
  • Elena Benavente
Short Communication

DOI: 10.1007/s10722-016-0364-5

Cite this article as:
Giraldo, P., Ruiz, M., Rodríguez-Quijano, M. et al. Genet Resour Crop Evol (2016) 63: 401. doi:10.1007/s10722-016-0364-5


The genus Aegilops includes a number of wheat wild relatives representing a valuable gene pool for stress adaptive traits. Collection of new accessions and proper management of Aegilops germplasm is thus essential for wheat improvement progress. Among the most worldwide distributed Aegilops species, A. geniculata Roth (2n = 4x = 28), A. neglecta Req. ex Bertol. (subsp. neglecta; 2n = 4x = 28) and A. recta (Zhuk.) Chen. (syn. A. neglecta Req. ex Bertol. subsp. recta (Zhuk.) Hammer; 2n = 6x = 42) are particularly difficult to distinguish each other because of their high morphological and genomic similarities. Based on their distinct cytoplasmic lineage, we have developed two chloroplast DNA-based molecular markers that accurately discriminate A. geniculata from A. neglecta and A. recta. The use of these markers, aided by chromosome counting to differentiate A. neglecta from A. recta, has allowed to assess the accuracy of species assignment in 125 accessions from Germplasm Genebank collections and recent collecting expeditions. This study has revealed taxonomic mistakes or inaccuracies in 18 % of the entries examined. The ambiguous use of the same species name for the allotetraploid A. neglecta and the allohexaploid A. recta, very extended among germplasm banks and managers, is in the origin of some of the errors detected.


Aegilops Chloroplast DNA markers Crop wild relatives Genetic resources management 

Supplementary material

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Supplementary material 1 (PPTX 264 kb)
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Supplementary material 5 (DOCX 115 kb)

Funding information

Funder NameGrant NumberFunding Note
National Institute for Agronomic Research (INIA)
  • RF2011-00018

Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Patricia Giraldo
    • 1
  • Magdalena Ruiz
    • 2
  • Marta Rodríguez-Quijano
    • 1
  • Elena Benavente
    • 1
  1. 1.Department of Biotechnology-Plant Biology, School of Agricultural EngineeringTechnical University of Madrid (UPM)MadridSpain
  2. 2.Plant Genetic Resources Centre (CRF)National Institute for Agricultural and Food Research and Technology (INIA)MadridSpain

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