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Current availability of seed material of enset (Ensete ventricosum, Musaceae) and its Sub-Saharan wild relatives

Abstract

Crop wild relatives are an important source of new genetic diversity for plant breeding and crop improvement. Enset (Ensete ventricosum) is mainly cultivated as a multipurpose crop in Ethiopia, supplying food to 13 million people. To improve the agronomic traits of enset clonal lines, sexual propagation can be of key importance, in particular crossbreeding landraces with populations of undomesticated Ensete wild relatives. We investigated the availability of stored seed material of the enset genepool represented by three African Ensete species (E. homblei, E. livingstonianum, E. ventricosum) in gene bank facilities across the world, and also the possibility to obtain freshly collected, undried seed material for conservation and research purposes. We queried the main plant genetic resources databases and contacted 27 gene and seed banks, research centres and several individual researchers. Only one seed bank seemed to hold a seed accession of only one of the three species, E. ventricosum (Millennium Seed Bank of the Royal Botanic Gardens, Kew). Available seed accessions of the other two species (E. homblei, E. livingstonianum) do not seem to exist. The lack of stored seed material of the three Ensete species and the difficulties in obtaining fresh seeds make it impossible to use seeds for breeding and crop improvement. We consider the existing seed conservation measures of the enset crop and its wild relatives as insufficient. A collection, research and ex situ conservation programme targeting Sub-Saharan Ensete seeds is needed considering the potential value of these species for food security across Africa.

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References

  • Amede T, Taboge E (2007) Chapter 26, Optimizing soil fertility gradients in the enset (Ensete ventricosum) systems of the Ethiopian highlands: trade-offs and local innovations. In: Bationo A, Waswa B, Kihara J, Kimetu J (eds) Advances in integrated soil fertility management in sub-Saharan Africa: challenges and opportunities. Springer, Dordrecht, pp 289–297

    Chapter  Google Scholar 

  • Asseng S, Ewert F, Martre P, Rötter RP, Lobell DB, Cammarano D, Kimball BA, Ottman MJ, Wall GW, White JW, Reynolds MP, Alderman PD, Prasad PVV, Aggarwal PK, Anothai J, Basso B, Biernath C, Challinor AJ, De Sanctis G, Doltra J, Fereres E, Garcia-Vila M, Gayler S, Hoogenboom G, Hunt LA et al (2015) Rising temperatures reduce global wheat production. Nat Clim Change 5:143–147

    Article  Google Scholar 

  • Baker RED, Simmonds NW (1953) The genus Ensete in Africa. Kew Bull 8(3):405–416

    Article  Google Scholar 

  • BGCI (2015) Botanic Garden Conservation International. www.bgci.org. Accessed 31 July 2015

  • Birmeta G, Nybom M, Bekele E (2004) Distinction between wild and cultivated enset (Ensete ventricosum) gene pools in Ethiopia using RAPD markers. Hereditas 140:139–148

    PubMed  Article  Google Scholar 

  • Castañeda-Álvarez NP, Khoury CK, Achicanoy HA, Bernau V, Dempewolf H, Eastwood RJ, Guarino L, Harker RH, Jarvis A, Maxted N, Müller JV, Ramirez-Villegas J, Sosa CC, Struik PC, Vincent H, Toll J (under review) Global conservation priorities for crop wild relatives. Nat Plants

  • Contu S (2013) Ensete livingstonianum. The IUCN red list of threatened species. Version 2015.2. www.iucnredlist.org. Downloaded 30 July 2015

  • Diro M, van Staden J (2003) In vitro regeneration of Ensete ventricosum from zygotic embryos of stored seeds. S Afr J Bot 69(3):364–369

    Article  Google Scholar 

  • Diro M, van Staden J (2004a) Propagation of Ensete in vitro: a review. S Afr J Bot 70(4):497–501

    Article  Google Scholar 

  • Diro M, van Staden J (2004b) Germination of zygotic embryos and in vitro growth of seedlings of wild types of Ensete ventricosum. S Afr J Bot 70(4):635–639

    Article  Google Scholar 

  • Emshwiller E, Calberto-Sànchez G, Girma G, Jansky S, Sardos J, Staver C, Stoddard FL, Roux N (2015) Chapter 13: Unavailability of wild relatives. In: Redden R, Yadav SS, Maxted N, Dulloo E, Guarino L, Smith P (eds) Crop wild relatives and climate change. Wiley Blackwell, Hoboken, pp 224–249

    Chapter  Google Scholar 

  • Esquinas-Alcázar J (2005) Protecting crop genetic diversity for food security: political, ethical and technical challenges. Nature 6:946–953

    Google Scholar 

  • EURISCO (2015) EURISCO: finding seeds for the future. http://eurisco.ipk-gatersleben.de. Accessed 31 July 2015

  • FAO (2009) International treaty on plant genetic resources for food and agriculture. Food and Agriculture Organization of the United Nations, Rome

    Google Scholar 

  • FAO WIEWS (2011) The world information and early warning system (WIEWS) on plant genetic resources for food and agriculture (PGRFA) established by FAO. http://www.fao.org/wiews-archive. Accessed 31 July 2015

  • GBIS/I (2006) Genebank information system of the IPK Gatersleben. http://gbis.ipk-gatersleben.de. Accessed 31 July 2015

  • Genesys (2015) Gateway to genetic resources. https://www.genesys-pgr.org/welcome. Accessed 31 July 2015

  • Godfray HCJ, Beddington JR, Crute IR, Haddad L, Lawrence D, Muir JF, Pretty J, Robinson S, Thomas SM, Toulmin C (2010) Food security: the challenge of feeding 9 billion people. Science 327:812–818

    PubMed  CAS  Article  Google Scholar 

  • GRIN (2012) United States Department of Agriculture, Agricultural Research Service, Germplasm Resources Information Network, National Plant Germplasm System. www.ars-grin.gov. Accessed 31 July 2015

  • Hajjar R, Hodgkin T (2007) The use of wild relatives in crop improvement: a survey of developments over the last 20 years. Euphytica 156:1–13

    Article  Google Scholar 

  • Hepper FN (1968) Musaceae. In: Hepper FN, Hutchinson J, Dalziel JM (eds) Flora of west tropical Africa, volume III part I. Royal Botanic Gardens, Kew, pp 67–69

    Google Scholar 

  • Karlsson LM, Tamado T, Dalbato AL, Mikias Y (2013) Seed morphology and germination of Ensete ventricosum (Musaceae). Seed Sci Technol 41:357–370

    Article  Google Scholar 

  • Karlsson LM, Dalbato AL, Tamado T, Mikias Y (2014) Effect of cultivar, traditional corm pre-treatment and watering on sprouting and early growth of enset (Ensete ventricosum) suckers. Exp Agric 51(2):232–243

    Article  Google Scholar 

  • Lebrun JP, Stork AL (2012) Tropical African flowering plants: ecology and distribution volume 7: monocotyledons 1 (Limnocharitaceae-Agavaceae). Conservatoire et Jardin botaniques de la Ville de Genève, Geneva

    Google Scholar 

  • Li LF, Häkkinen M, Yuan YM, Hao G, Ge XJ (2010) Molecular phylogeny and systematics of the banana family (Musaceae) inferred from multiple nuclear and chloroplast DNA fragments, with a special reference to the genus Musa. Mol Phylogenet Evol 57:1–10

    PubMed  Article  Google Scholar 

  • Liu AZ, Kress WJ, Li DZ (2010) Phylogenetic analyses of the banana family (Musaceae) based on nuclear ribosomal (ITS) and chloroplast (trnL-F) evidence. Taxon 59:20–28

    Google Scholar 

  • Lock JM (1993) Musaceae. In: Polhill RM (ed) Flora of tropical east Africa. Royal Botanic Gardens, Kew

    Google Scholar 

  • Lock JM, Diniz MA (2010) Musaceae. In: Timberlake JR, Martins ES (eds) Flora Zambesiaca, vol 13 part four. Royal Botanic Gardens, Kew, pp 104–111

    Google Scholar 

  • McCouch S, Baute GJ, Bradeen J, Bramel P, Bretting PK, Buckler E, Burke JM, Charest D, Cloutier S, Cole G, Dempewolf H, Dingkuhn M, Feuillet C, Gepts P, Grattapaglia D, Guarino L, Jackson S, Knapp S, Langridge P, Lawton-Rauh A, Lijua Q, Lusty C, Michael T, Myles S, Naito K, Nelson RL, Pontarollo R, Richards CM, Rieseberg L, Ross-Ibarra J, Rounsley S, Hamilton RS, Schurr U, Stein N, Tomooka N, van der Knaap E, van Tassel D, Toll J, Valls J, Varshney RK, Ward J, Waugh R, Wenzl P, Zamir D (2013) Feeding the future. Nature 499:23–24

    PubMed  CAS  Article  Google Scholar 

  • Mohammed B, Gabel M, Karlsson LM (2013) Nutritive values of the drought tolerant food and fodder crop enset. Afr J Agric Res 8(20):2326–2333

    Google Scholar 

  • Negash A (2001) Diversity and conservation of Enset (Ensete ventricosum (Welw.) Cheesman) and its relation to household food and livelihood security in South-western Ethiopia. PhD Thesis, Wageningen University

  • Negash A, Puite K, Schaart J, Visser B, Krens F (2000) In vitro regeneration and micro-propagation of enset from Southwestern Ethiopia. Plant Cell Tissue Organ 62:153–158

    Article  Google Scholar 

  • Olango TM, Tesfaye B, Catellani M, Pè ME (2014) Indigenous knowledge, use and on-farm management of enset (Ensete ventricosum (Welw.) Cheesman) diversity in Wolaita, Southern Ethiopia. J Ethnobiol Ethnomed 10:41

    PubMed  PubMed Central  Article  Google Scholar 

  • Shigeta M (1990) Folk in situ conservation of Ensete (Ensete ventricosum (Welw.) E.E. Cheesman): towards the interpretation of indigenous agricultural science of the Ari Southwestern Ethiopia. Afr Stud Monogr 10(3):93–107

    Google Scholar 

  • SID MSBP (2008) Seed information database of the Millennium Seed Bank Partnership. http://data.kew.org/sid. Accessed 31 July 2015

  • Tesfaye M (1992) The effect of soaking, temperature and other pretreatments on the germination of ‘enset’ seed. Seed Sci Technol 20:533–538

    Google Scholar 

  • Tsegaye A, Struik PC (2002) Analysis of enset (Ensete ventricosum) indigenous production methods and farm-based biodiversity in major enset-growing regions in southern Ethiopia. Exp Agric 38:291–315

    Article  Google Scholar 

  • Tsegaye A, Westphal E (2002) Ensete ventricosum (Welw.) Cheesman. In: Oyen LPA, Lemmens RHM (eds) PROTA4U. PROTA (Plant Resources of Tropical Africa/Ressources végétales de l’Afrique tropicale), Wageningen. http://www.prota4u.org/search.asp. Accessed 31 July 2015

  • VIR database (2015) Plant genetic resources database of the Vavilov Institute of Plant Industry. http://91.151.189.38/virdb. Accessed 31 July 2015

  • Warschefsky E, Varma Penmetsa R, Cook DR, von Wettemberg EJB (2014) Back to the wild: tapping evolutionary adaptations for resilient crops through systematic hybridization with crop wild relatives. Am J Bot 101(10):1791–1800

    PubMed  Article  Google Scholar 

Download references

Acknowledgments

The authors would like to thank Ruth Eastwood for her help in searching the databases, Thomas Abeli, Luigi Guarino and two anonymous reviewers for their valuable comments on an earlier draft of the manuscript.

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Correspondence to Jonas V. Müller.

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Guzzon, F., Müller, J.V. Current availability of seed material of enset (Ensete ventricosum, Musaceae) and its Sub-Saharan wild relatives. Genet Resour Crop Evol 63, 185–191 (2016). https://doi.org/10.1007/s10722-015-0355-y

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  • DOI: https://doi.org/10.1007/s10722-015-0355-y

Keywords

  • Crop wild relatives
  • Ensete
  • Ex situ conservation
  • Food security
  • Germplasm
  • Plant breeding
  • Seeds