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Genetic Resources and Crop Evolution

, Volume 64, Issue 2, pp 277–290 | Cite as

Patterns of SSR variation in bread wheat (Triticum aestivum L.) seeds under ex situ genebank storage and accelerated ageing

  • Yong-Bi Fu
  • Mo-Hua Yang
  • Carolee Horbach
  • Dallas Kessler
  • Axel Diederichsen
  • Frank M. You
  • Hong Wang
Research Article

Abstract

Maintaining seed viability and germplasm integrity is a challenging task in conservation of plant genetic resources, as seeds under storage will lose viability and genetic changes will occur. Attempt was made to analyze the patterns of genetic changes in wheat germplasm under ex situ genebank storage and accelerated ageing treatments. A set of 16 naturally aged wheat accessions under ex situ genebank storage since 1994 were sampled. Four recently regenerated wheat accessions were selected, four random seed samples were chosen from each accession, and three of them were exposed to three different accelerated ageing treatments. These 32 seed samples in two germplasm sets displayed a range of germination rates from 4 to 98 %. Thirty-seven microsatellite markers representing 21 wheat chromosomes were applied to screen 12 seeds of each sample and 449 SSR alleles were scored. Large SSR variation was found in each germplasm set. There was 73.1 % of the total SSR variation present among the naturally aged samples and 78.2 % present among the accelerated ageing samples. Several analyses for genetic association consistently revealed no clear genetic separations among samples of high or low germination rates in both germplasm sets. Samples under different accelerated ageing treatments did not show much genetic differentiations from the original sample of each accession. Mantel tests revealed non-significant associations between SSR variability and sample germination rates for both germplasm sets. These findings are useful for understanding seed deterioration under different ageing conditions and suggest that genome-wide SSR variability may not provide sensitive markers for the monitoring of wheat seed viability.

Keywords

Accelerated ageing Ex situ conservation Microsatellite marker Seed storage Seed viability Triticum aestivum Viability marker 

Notes

Acknowledgments

Authors would like to thank Lorie Jones-Flory, Gregory Peterson, and Yasmina Bekkaoui for technical assistance in germination testing; Vicky Roslinsky for access to ABI sequencer for SSR analysis; and two anonymous journal reviewers for their helpful comments on the early version of the manuscript. This research was financially supported by an A-Base research project of Agriculture and Agri-Food Canada to YBF, and the China Scholarship Council Postdoctoral Abroad Grant to M-HY.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© Her Majesty the Queen in Right of Canada 2015

Authors and Affiliations

  • Yong-Bi Fu
    • 1
  • Mo-Hua Yang
    • 1
    • 2
  • Carolee Horbach
    • 1
  • Dallas Kessler
    • 1
  • Axel Diederichsen
    • 1
  • Frank M. You
    • 3
  • Hong Wang
    • 4
  1. 1.Plant Gene Resources of Canada, Saskatoon Research and Development CentreAgriculture and Agri-Food CanadaSaskatoonCanada
  2. 2.College of ForestryCentral South University of Forestry and TechnologyChangshaChina
  3. 3.Morden Research and Development CentreAgriculture and Agri-Food CanadaMordenCanada
  4. 4.Department of BiochemistryUniversity of SaskatchewanSaskatoonCanada

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