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Genetic diversity and structure of teak (Tectona grandis L. f.) and dahat (Tectona hamiltoniana Wall.) based on chloroplast microsatellites and Amplified Fragment Length Polymorphism markers

Genetic Resources and Crop Evolution volume 63, pages 961–974 (2016)Cite this article

Abstract

Teak (Tectona grandis L. f.) is a tropical forest tree species naturally occurring in India, Laos, Myanmar and Thailand. The closely related dahat (Tectona hamiltoniana Wall.) is an endemic tree species confined to Myanmar. Two chloroplast Simple Sequence Repeat (cpSSR) and sixty-nine Amplified Fragment Length Polymorphism (AFLP) markers were applied to assess patterns of genetic variation in four T. grandis and three T. hamiltoniana populations in Myanmar. The cpSSR analysis confirmed a clear genetic differentiation between species, revealing a single haplotype (H1) in T. grandis, while the three T. hamiltoniana populations were fixed on different haplotypes (H2 or H3). AFLP analysis revealed that genetic diversity varied between species, showing a slightly higher variation in T. grandis than in T. hamiltoniana. The T. hamiltoniana populations showed similar levels of genetic variation, while parameters varied considerably in T. grandis populations. Analyses of Molecular Variance revealed significant genetic differentiation between the two species (38.4 %, p < 0.05) and among populations within species. Genetic variation mainly resided within populations. Significant pairwise genetic differentiation (pairwise F ST ) was detected between most populations (p < 0.05). An Unweighted Pair Group Method with Arithmetic Mean dendrogram based on Nei’s genetic distances revealed a clear genetic differentiation between species. Diagnostic AFLP markers with complete or nearly complete differentiation between species and complete differentiation at cpDNA markers indicated strong phylogenetic divergence between teak and dahat.

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Acknowledgments

The study was conducted with scholarship support from German Academic Exchange Services for the first author’s master degree course. Technical assistances from Olga Artes and Alexandra Dolnyska are greatly appreciated.

Funding

The study was funded by German Academic Exchange Services. The first author received a scholarship from German Academic Exchange Services for his master degree. The second and third authors supervised the study.

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Authors and Affiliations

  1. Department of Forest Genetics and Forest Tree Breeding, Georg-August University Göttingen, Büsgenweg 2, 37077, Göttingen, Germany

    Yazar Minn, Oliver Gailing & Reiner Finkeldey

  2. Forest Botany and Tree Improvement Section, Forest Research Institute, 05282, Yezin, Nay Pyi Taw, Myanmar

    Yazar Minn

  3. School of Forest Resources and Environmental Science, Michigan Technological University, 1400 Townsend Drive, Houghton, MI, 49931-1295, USA

    Oliver Gailing

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  1. Yazar Minn
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Correspondence to Yazar Minn.

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Minn, Y., Gailing, O. & Finkeldey, R. Genetic diversity and structure of teak (Tectona grandis L. f.) and dahat (Tectona hamiltoniana Wall.) based on chloroplast microsatellites and Amplified Fragment Length Polymorphism markers. Genet Resour Crop Evol 63, 961–974 (2016). https://doi.org/10.1007/s10722-015-0293-8

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Keywords

  • AFLPs
  • cpSSRs
  • Genetic variation
  • Myanmar
  • Tectona grandis
  • Tectona hamiltoniana

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