Skip to main content
SpringerLink
Log in

Phenotypic and genotypic characterization in the collection of sour and duke cherries (Prunus cerasus and ×P. ×gondouini) of the Fruit Genebank in Dresden-Pillnitz, Germany

Genetic Resources and Crop Evolution volume 62, pages 551–566 (2015)Cite this article

Abstract

Phenotypic and genotypic characterization of existing sour and duke cherry cultivars provide information for use by breeders in developing cultivars with improved qualities and for germplasm management to optimise the conserved diversity. This study examined 79 sour and duke cherry cultivars of the Fruit Genebank of the Institute for Breeding Research on Fruit Crops, Dresden-Pillnitz. For morphological analysis, 23 descriptors of the tree, the flower or the fruit, and three phenological characters were recorded to detect similarities and differences among the cultivars. Most of the characteristics exhibited a high degree of variability. Principal component analysis revealed that the first three components were responsible for 48 % of the phenotypic variability. Color descriptors as well as the fruit size and weight, the organoleptic quality and the two descriptors related to the abscission between fruit and stalk were the most effective parameters to discriminate genotypes. The genetic analysis was performed using six microsatellite markers recommended by the European Cooperative Program for Plant Genetic Resources. The average number of the effective alleles was 7.11 and the expected heterozygosity ranged from 0.78 to 0.89. The highest expected heterozygosity was achieved with the primer pairs EMPaS10 and EMPaS12. The tree comparison (‘bipartition distance’) estimated by the two data sets, morphological and genetic, revealed a tree distance of 0.669. Apparently, more research is needed for combining and jointly interpreting phenotypic and genetic marker data in diversity analysis for plant breeding and germplasm management.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Fig. 1
Fig. 2

References

  • Antonius K, Aaltonen M, Uosukainen M, Hurme T (2012) Genotypic and phenotypic diversity in Finnish cultivated sour cherry (Prunus cerasus L.). Genet Resour Crop Evol 59:375–388

    Article  Google Scholar 

  • Asma BM, Ozturk K (2005) Analysis of morphological, pomological and yield characteristics of some apricot germplasm in Turkey. Genet Resour Crop Evol 52:305–313

    Article  Google Scholar 

  • Badenes ML, Martinez-Calvo J, Llace G (1998) Analysis of apricot germplasm from the European ecogeographical group. Euphytica 102:93–99

    Article  Google Scholar 

  • Beaver JA, Iezzoni AF, Ramm CW (1995) Isozyme diversity in sour, sweet and ground cherry. Theor Appl Genet 90:847–852

    Article  CAS  PubMed  Google Scholar 

  • Byrne DH, Nikolic AN, Burns EE (1991) Variability in sugar, acids, firmness, and color characteristics of 12 peach genotypes. J Am Soc Hort Sci 116:1004–1006

    CAS  Google Scholar 

  • Cantini C, Iezzoni AF, Lamboy WF, Boritzki M, Struss D (2001) DNA fingerprinting of tetraploid cherry germplasm using simple sequence repeats. J Am Soc Hort Sci 126:205–209

    CAS  Google Scholar 

  • Cipriani G, Lot G, Huang WG, Marrazzo MT, Peterlunger E, Testolin R (1999) AC/GT and AG/CT microsatellite repeats in peach [Prunus persica (L.) Batsch]: isolation, characterization and cross-species amplification in Prunus. Theor Appl Genet 99:65–72

    Article  CAS  Google Scholar 

  • Clarke JB, Tobutt KR (2009) A standard set of accessions, mircosatellites and genotypes for harmonizing the fingerprinting of cherry collections for the ECPGR. Acta Hort 814:615–618

    Article  Google Scholar 

  • Demir T, Demirsoy L, Demirsoy H, Kacar YA, Yilmaz M, Macit I (2011) Molecular characterization of sweet cherry genetic resources in Giresun, Turkey. Fruits 66:53–62

    Article  CAS  Google Scholar 

  • Downey SL, Iezzoni AF (2000) Polymorphic DNA markers in black cherry (Prunus serotina) are identified using sequences from sweet cherry, peach and sour cherry. J Am Soc Hort Sci 125:76–80

    CAS  Google Scholar 

  • Flachowsky H, Höfer M (2010) Die Deutsche Genbank Obst, ein dezentrales Netzwerk zur nachhaltigen Erhaltung genetischer Ressourcen bei Obst. Journal für Kulturpflanzen 62:9–16

    Google Scholar 

  • Ganopoulos IV, Kazantzis K, Chantzicharisis I, Karayiannis I, Tsaftaris AS (2011) Genetic diversity, structure and fruit trait associations in Greek sweet cherry cultivars using microsatellite based (SSR/ISSR) and morpho-physiological markers. Euphytica 181:237–251

    Article  Google Scholar 

  • Gascuel O (1997) Concerning the NJ algorithm and its unweighted version, UNJ. In: Mirkin B, McMorris F, Roberts F, Rzetsky A (eds) Mathematical hierarchies and biology. American Mathematical Society, DIMACS workshop, vol 37, pp 149–170

  • Gasi F, Simon S, Posjskic N, Kurtovic M, Pejic I (2010) Genetic assessment of apple germplasm in Bosnia and Herzegovina using microsatellite and morphologic markers. Sci Hortic 126:164–171

    Article  Google Scholar 

  • Gerlach HK, Stosser R (1998) Sweet cherry cultivar indentification using RAPD-derived DNA fingerprints. Acta Hort 468:63–69

    Article  CAS  Google Scholar 

  • Guarino C, Santoro S, De Simone L, Cipriani G (2009) Prunus avium: nuclear DNA study in wild populations and sweet cherry cultivars. Genome 52:320–337

  • Guarino C, Santoro S, De Simone L, Cipriani G (2010) Molecular characterization of ancient Prunus avium L. germplasm using sweet cherry SSR markers. J Horticultural Sci Biotechnol 85:295–304

    Article  CAS  Google Scholar 

  • Gulen H, Ipek A, Ergin S, Akacay ME, Eris A (2010) Assessment of genetic relationships among 29 introduced and 49 local sweet cherry accessions in Turkey using AFLP and SSR markers. J Hortic Sci Biotechnol 85:427–431

    Article  CAS  Google Scholar 

  • Hanelt P, Institute of Plant Genetics and Crop Plant Research (2001) Mansfeld’s Encyclopedia of Agricultural and Horticultural Crops. Springer, Berlin, pp 504–508

  • Hillig KW, Iezzoni AF (1988) Multivariate analysis of a sour cherry germplasm collection. J Am Soc Hort Sci 113:928–934

    Google Scholar 

  • Höfer M, Drewes-Alwarez R, Scheewe P, Olbricht K (2012) Morphological evaluation of 108 strawberry cultivars—and consequences for the use of descriptors. J Berry Res 2:191–206. doi:10.3233/JBR-2012-042

    Google Scholar 

  • IBPGR (1985) Cherry descriptor list. International Board for plant genetic resources, Rome

    Google Scholar 

  • Iezzoni AF, Schmidt H, Albertini A (1990) Cherries (Prunus). In: Moore JN, Bellington Jr. JR (eds) Genetic resources of temperate fruit and nut crops. International Society for Horticultural Science, Vol 1. Wageningen, The Netherlands, pp. 109-173

  • Iezzoni AF, Mulinix CA (1992) Variation in bloom time in a sour cherry germplasm collection. HortScience 27:1113–1114

    Google Scholar 

  • Kacar Y, Cetiner MS, Cantini C, Iezzoni AF (2006) Simple Sequence Repaet (SSR) markers differentiate Turkish sour cherry germplasm. J Am Pomol Soc 60:136–143

    Google Scholar 

  • Kappel F, Granger A, Hrotko K, Schuster M (2012) Cherry. In: Bladenes ML, Byrne DH (eds) Fruit breeding, handbook of plant breeding, vol 8, Springer, Berlin, pp 459–504

  • Kaufman L, Rosseeuw PJ (1990) Finding groups in data. An introduction to cluster analysis. Wiley, New York

    Google Scholar 

  • Khadivi-Khub A, Zamani Z, Bouzan N (2008) Evaluation of genetic diversity in some iranian and foreign sweet cherry cultivars by using molecular markers and morphological traits. Hort Environ Biotechnol 49:188–196

    CAS  Google Scholar 

  • Khadivi-Khub A, Zamani Z, Fatahi MR (2012) Multivariate analysis of Prunus subgen. Cerasus germplasm in Iran using morphological variables. Genet Resour Crop Evol 59:909–926

    Article  Google Scholar 

  • Khadivi-Khub A, Jafari H-R, Zamani Z (2013) Phenotypic and genotypic variation in Iranian sour and duke cherries. Trees 27:1455–1466

    Article  Google Scholar 

  • Kobayashi N, Horikoshi T, Katsuyama H, Handa T, Takayanagi K (1998) A simple and efficient DNA extraction method for plants especially woody plants. Plant Tissue Cult Biotechnol 4:76–80

    Google Scholar 

  • Krahl KH, Lansari A, Iezzoni AF (1991) Morphological variation within a sour cherry collection. Euphytica 52:47–55

    Google Scholar 

  • Laurentin H (2009) Data analysis for molecular characterization of plant genetic resources. Genet Resour Crop Evol 56:277–292

    Article  CAS  Google Scholar 

  • Lisek A, Rozpara E (2009) Identification and genetic diversity assessment of cherry cultivars and rootstocks using ISSR-PCR technique. J Fruit Ornam Plant Res 17:95–106

    CAS  Google Scholar 

  • Marchese A, Tobutt KR, Raimondo A, Motisi A, Boskovic RI, Clarke J, Caruso T (2007) Morphological characteristics, microsatellite fingerprinting and determination of incompatibility genotypes of Sicilian sweet cherry cultivars. J Hortic Sci Biotechnol 82:41–48

    Article  CAS  Google Scholar 

  • Martinez L, Cavagnaro P, Masuelli R, Rodriguez J (2003) Evaluation of diversity among Argentine grapevine (Vitis vinifera L.) varieties using morphological data and AFLP markers. Electron J Biotechnol 6:241–250

    Article  Google Scholar 

  • Mnejja M, Garcia-Mas J, Audergon J-M, Arus P (2010) Prunus microsatellite marker transferability across rosaceous crops. Tree Genetics Genomes 6:689–700

    Article  Google Scholar 

  • Nazari SA, Zamani Z, Fatahi MR (2012) Morphological characterization of Prunus incana Pall. By multivariate analysis. Plant Syst Evol 298:1805–1814

    Article  Google Scholar 

  • Nikolic D, Rakonjac V, Milatovic D, Fotiric M (2010) Multivariate analysis of vineyard peach [Prunus persica (L.) Batsch.] germplasm collection. Euphytica 171:227–234

    Article  Google Scholar 

  • Pedersen BH (2006) DNA fingerprints of 51 sweet and sour Prunus accessions using simple sequence repeats. J Hortcult Sci Biotechnol 81:118–124

    Article  CAS  Google Scholar 

  • Perez R, Navarro F, Sanchaz MA, Ortiz JM (2010) Analysis of agronmorphological descriptors to differentiate between duke cherry (P. × gondouinii (Point. & Turpin) Rehd.) and its progenitors: sweet cherry (Prunus avium L.) and sour cherry (Prunus cerasus L.). Chil J Agric Res 70:34–49

    Google Scholar 

  • Perez-Sanchez R, Gomez-Sanchez MA, Morales-Corts R (2008) Agromorphological characterization of traditional sweet cherry (Prunus avium L.), sour cherry (Prunus cerasus L.) and duke cherry (P. × gondouinii Rehd.) cultivars. Span J Agric Res 6:42–55

    Article  Google Scholar 

  • Perrier X, Jacquemoud-Collet JP (2006) DARwin software. http://www.darwin.cirad.fr/darwin

  • Perz S, Montes S, Mejia C (1993) Analysis of peach germplasm in Mexico. J Am Soc Hort Sci 118:519–524

    Google Scholar 

  • Rakonjac V, Aksic MF, Nikolic D, Milatovic D, Colic S (2010) Morphological characterization of ‘Oblacinska’ sour cherry by multivariate analysis. Sci Hortic 125:679–684

    Article  Google Scholar 

  • Rehder A (1949) Bibliography of cultivated trees and shrubs. The Arnold Arboretum of Harvard University, Jamaica Plain, Massachusetts

  • Rodrigues LC, Morales MR, Fernandes AJB, Ortiz JM (2008) Morphological characterization of sweet and sour cherry cultivars in a germplasm bank in Portugal. Genet Resour Crop Evol 55:593–601

    Article  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method—a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  • Shahi-Gharahlar A, Zamani Z, Fatahi MR, Bouzari N (2010) Assessment of morphological variation between some Iranian wild Cerasus sub-genus genotypes. Hort Environ Biotechnol 51:308–318

    Google Scholar 

  • Shahi-Gharahlar A, Zamani Z, Fatahi MR, Bouzari N (2011) Estimation of genetic diversity in some Iranian wild Prunus subgenus Cerasus accessions using inter-simple sequence repeat (ISSR) markers. Biochem Syst Ecol 39:826–833

    Article  CAS  Google Scholar 

  • Shimada T, Hayama H, Nishimura K, Yamaguchi M, Yoshida M (2001) The genetic diversities of 4 species of subg. Lithocerasus (Prunus, Rosaceae) revealed by RAPD analysis. Euphytica 117:85–90

    Article  Google Scholar 

  • Stanys V, Frercks B, Siksnianiene JB, Stepulaitiene I, Gelvonauskiene D, Staniene G, Bobinas C (2012) Identification of sweet cherry (Prunus avium L.) cultivars using AFLP and SSR markers. Zemdirb Agric 99:437–444

    Google Scholar 

  • Struss D, Boritzki M, Glozer K, Southwick SM (2001) Detection of genetic diversity among populations of sweet cherry (Prunus avium L.) by AFLPs. J Hort Sci Biotechnol 76:362–367

    Article  CAS  Google Scholar 

  • Struss D, Ahmad R, Southwick SM (2003) Analysis of sweet cherry (Prunus avium L.) cultivars using SSR and AFLP markers. J Am Soc Hort Sci 128:904–909

    CAS  Google Scholar 

  • Tavaud M, Zanetto A, David JL, Laigret F, Dirlewanger E (2004) Genetic relationships between diploid and allotetraploid cherry species (Prunus avium, Prunus × gondouinii and Prunus cerasus). Heredity 93:631–638

    Article  CAS  PubMed  Google Scholar 

  • Tobutt KR, Boskovic R (1996) A cherry gene database. Acta Hort 410:147–153

    Article  Google Scholar 

  • Toydemir G, Capanoglu E, Kamiloglu S, Boyacioglu D, de Vos R, Hall RD, Beekwilder J (2013) Changes in sour cherry (Prunus cerasus L.) antioxidants during nectar processing and in vitro gastrointestinal. J Funct Food 5:1402–1413

    Article  CAS  Google Scholar 

  • UPOV (1995) Guidelines for the conduct of tests for distinctness, uniformity and stability (Cherry). TG/35/6

  • Usenik V, Stampar F (2011) The effect of environmental temperature on sweet cherry phenology. Europ. Hort. Sci. 76:1–5

    Google Scholar 

  • Van Tienderen PH, de Haan AA, van der Linden CG, Vosman B (2002) Biodiversity assessment using markers for ecologically important traits. Trend Ecol Evol 17:577–582

    Article  Google Scholar 

  • Vaughan SP, Russell K (2004) Characterization of novel microsatellites and development of multiplex PCR for large-scale population studies in wild cherry, Prunus avium. Mol Ecol Notes 4:429–431

    Article  CAS  Google Scholar 

  • Vavilov NI (1951) The origin, variation, immunity and breeding of cultivated plants. Ronald, New York

    Google Scholar 

  • Wang DR, Karle R, Iezzoni AF (2000) QTL analysis of flower and fruit traits in sour cherry. Theor Appl Genet 100:535–544

    Article  CAS  Google Scholar 

  • Webster AD, Schmidt H (1996) Rootstocks for sweet and sour cherries. In: Webster AD, Looney NE (eds) Cherries: crop physiology, production and uses. CAB International Press, Wallingford, UK, pp 127–167

  • Wünsch A, Hormaza JL (2004) Molecular evaluation of genetic diversity and S-allele composition of local Spanish sweet cherry (Prunus avium L.) cultivars. Genet Resour Crop Evol 51:635–641

    Article  Google Scholar 

  • Xuan H, Wang R, Büchele M, Möller O (2009) Microsatellite markers (SSR) as a tool to assist in identification of sweet (Prunus avium) and sour cherry (Prunus cerasus). Acta Hort 839:507–514

    Article  CAS  Google Scholar 

  • Yilmaz KU, Ercisli S, Asma BM, Dogan Y, Kafkas S (2009) Genetic relatedness in Prunus genus revealed by inter simple sequence repeat markers. HortScience 44:293–297

    Google Scholar 

  • Zamani Z, Sarkhosh A, Fatahi R, Ebadi A (2007) Genetic relationships among pomegranate genotypes studied by fruit characteristics and RAPD markers. J Hortic Sci Biotechnol 82:11–18

    Article  CAS  Google Scholar 

  • Zamani Z, Shahi-Gharahlar A, Fatahi R, Bouzari N (2012) Genetic relatedness among some wild cherry (Prunus subgenus Cerasus) genotypes native to Iran assayed by morphological traits and random amplified polymorphic DNA analysis. Plant Syst Evol 298:499–509. http://faostat.fao.org/site/339/default.aspx

Download references

Author information

Authors and Affiliations

  1. Julius Kühn-Institute, Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Fruit Crops, Pillnitzer Platz 3a, 01326, Dresden, Germany

    Monika Höfer & Andreas Peil

Authors
  1. Monika Höfer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Andreas Peil
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Monika Höfer.

Rights and permissions

Reprints and Permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Höfer, M., Peil, A. Phenotypic and genotypic characterization in the collection of sour and duke cherries (Prunus cerasus and ×P. ×gondouini) of the Fruit Genebank in Dresden-Pillnitz, Germany. Genet Resour Crop Evol 62, 551–566 (2015). https://doi.org/10.1007/s10722-014-0180-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10722-014-0180-8

Keywords

search

Navigation