Genetic Resources and Crop Evolution

, Volume 62, Issue 1, pp 103–114 | Cite as

Molecular confirmation of species status for the allopolyploid cotton species, Gossypium ekmanianum Wittmack

  • C. E. Grover
  • X. Zhu
  • K. K. Grupp
  • J. J. Jareczek
  • J. P. Gallagher
  • E. Szadkowski
  • J. G. Seijo
  • J. F. Wendel
Research Article

Abstract

Understanding the relationship between domesticated crop species and their wild relatives is paramount to germplasm maintenance and the utilization of wild relatives in breeding programs. Recently, Gossypium ekmanianum was resurrected as an independent species based on morphological analysis of specimens obtained from the Dominican Republic, where the original type specimen was collected. The molecular data presented here support the recognition of G. ekmanianum Wittmack as a distinct species that is phylogenetically close to G. hirsutum L. Analyses of chloroplast DNA data reveal species-specific, indel polymorphisms that unambiguously distinguish G. ekmanianum samples from other polyploid congeners. Furthermore, analysis of accessions that originated from the Dominican Republic demonstrate the cryptic inclusion of this sister taxon within the US National Plant Germplasm System, a germplasm collection maintained for diversity preservation and future breeding resources. The data presented here indicate that “wild” G. hirsutum accessions may include the closely related G. ekmanianum, and provide a method to easily distinguish the two.

Keywords

Breeding Cryptic species Germplasm Gossypium ekmanianum Polyploid 

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • C. E. Grover
    • 1
  • X. Zhu
    • 2
  • K. K. Grupp
    • 1
  • J. J. Jareczek
    • 1
  • J. P. Gallagher
    • 1
  • E. Szadkowski
    • 1
  • J. G. Seijo
    • 3
  • J. F. Wendel
    • 1
  1. 1.Department of Ecology, Evolution, and Organismal BiologyIowa State UniversityAmesUSA
  2. 2.School of Life SciencesNantong UniversityNantongChina
  3. 3.Instituto de Botánica del Nordeste (UNNE-CONICET) and Facultad de Ciencias Exactas y Naturales y AgrimensuraUniversidad Nacional del NordesteCorrientesArgentina

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